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- W2088616392 endingPage "e59567" @default.
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- W2088616392 abstract "Hot spot mutant p53 (mutp53) proteins exert oncogenic gain-of-function activities. Binding of mutp53 to DNA is assumed to be involved in mutp53-mediated repression or activation of several mutp53 target genes. To investigate the importance of DNA topology on mutp53-DNA recognition in vitro and in cells, we analyzed the interaction of seven hot spot mutp53 proteins with topologically different DNA substrates (supercoiled, linear and relaxed) containing and/or lacking mutp53 binding sites (mutp53BS) using a variety of electrophoresis and immunoprecipitation based techniques. All seven hot spot mutp53 proteins (R175H, G245S, R248W, R249S, R273C, R273H and R282W) were found to have retained the ability of wild-type p53 to preferentially bind circular DNA at native negative superhelix density, while linear or relaxed circular DNA was a poor substrate. The preference of mutp53 proteins for supercoiled DNA (supercoil-selective binding) was further substantiated by competition experiments with linear DNA or relaxed DNA in vitro and ex vivo. Using chromatin immunoprecipitation, the preferential binding of mutp53 to a sc mutp53BS was detected also in cells. Furthermore, we have shown by luciferase reporter assay that the DNA topology influences p53 regulation of BAX and MSP/MST1 promoters. Possible modes of mutp53 binding to topologically constrained DNA substrates and their biological consequences are discussed." @default.
- W2088616392 created "2016-06-24" @default.
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- W2088616392 date "2013-03-26" @default.
- W2088616392 modified "2023-10-17" @default.
- W2088616392 title "Preferential Binding of Hot Spot Mutant p53 Proteins to Supercoiled DNA In Vitro and in Cells" @default.
- W2088616392 cites W1456925158 @default.
- W2088616392 cites W1522967486 @default.
- W2088616392 cites W1594073581 @default.
- W2088616392 cites W1870851597 @default.
- W2088616392 cites W1947531295 @default.
- W2088616392 cites W1952836109 @default.
- W2088616392 cites W1963972559 @default.
- W2088616392 cites W1964178865 @default.
- W2088616392 cites W1966082941 @default.
- W2088616392 cites W1967058595 @default.
- W2088616392 cites W1968075505 @default.
- W2088616392 cites W1976921587 @default.
- W2088616392 cites W1978035537 @default.
- W2088616392 cites W1979268424 @default.
- W2088616392 cites W1979607371 @default.
- W2088616392 cites W1984018823 @default.
- W2088616392 cites W1986130304 @default.
- W2088616392 cites W1988332041 @default.
- W2088616392 cites W1988894877 @default.
- W2088616392 cites W1991234732 @default.
- W2088616392 cites W1995177916 @default.
- W2088616392 cites W1995637638 @default.
- W2088616392 cites W1999060423 @default.
- W2088616392 cites W1999760670 @default.
- W2088616392 cites W2002505344 @default.
- W2088616392 cites W2005389118 @default.
- W2088616392 cites W2005955161 @default.
- W2088616392 cites W2007978091 @default.
- W2088616392 cites W2010363079 @default.
- W2088616392 cites W2013969883 @default.
- W2088616392 cites W2017449972 @default.
- W2088616392 cites W2017666786 @default.
- W2088616392 cites W2019375321 @default.
- W2088616392 cites W2022909023 @default.
- W2088616392 cites W2028640227 @default.
- W2088616392 cites W2032045214 @default.
- W2088616392 cites W2033176861 @default.
- W2088616392 cites W2036179093 @default.
- W2088616392 cites W2038619560 @default.
- W2088616392 cites W2042484101 @default.
- W2088616392 cites W2043985316 @default.
- W2088616392 cites W2045887660 @default.
- W2088616392 cites W2053025410 @default.
- W2088616392 cites W2053462331 @default.
- W2088616392 cites W2054418122 @default.
- W2088616392 cites W2065195919 @default.
- W2088616392 cites W2066721703 @default.
- W2088616392 cites W2069452349 @default.
- W2088616392 cites W2070062778 @default.
- W2088616392 cites W2070975347 @default.
- W2088616392 cites W2073576377 @default.
- W2088616392 cites W2074624243 @default.
- W2088616392 cites W2080729278 @default.
- W2088616392 cites W2086332776 @default.
- W2088616392 cites W2089106375 @default.
- W2088616392 cites W2089287100 @default.
- W2088616392 cites W2093443539 @default.
- W2088616392 cites W2094957670 @default.
- W2088616392 cites W2095438551 @default.
- W2088616392 cites W2099459640 @default.
- W2088616392 cites W2105781930 @default.
- W2088616392 cites W2106334971 @default.
- W2088616392 cites W2108051607 @default.
- W2088616392 cites W2111523470 @default.
- W2088616392 cites W2111705979 @default.
- W2088616392 cites W2112396116 @default.
- W2088616392 cites W2123786201 @default.
- W2088616392 cites W2123868608 @default.
- W2088616392 cites W2125602642 @default.
- W2088616392 cites W2130956190 @default.
- W2088616392 cites W2144963939 @default.
- W2088616392 cites W2148119650 @default.
- W2088616392 cites W2149439920 @default.
- W2088616392 cites W2149917234 @default.
- W2088616392 cites W2153995881 @default.
- W2088616392 cites W2156406934 @default.
- W2088616392 cites W2162487261 @default.
- W2088616392 cites W2168087339 @default.
- W2088616392 cites W2171766579 @default.
- W2088616392 cites W2322456862 @default.
- W2088616392 cites W2332353899 @default.