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- W2088643445 abstract "Abstract Slices of rachitic growth plate cartilage have proven a difficult tissue in which to study 45Ca uptake because, even in rickets, there exists an area of preformed mineral at the base of the growth plate. This extraneous mineral accumulates 45Ca through exchange and apatite crystal proliferation when exposed to a calcifying solution, thus creating a background of radioactivity which obscures 45Ca uptake into vesicle-initiated mineral. We have succeeded in removing the preformed mineral, and hence much background radioactivity by exposing slices of rachitic rat epiphyseal plate to 30 mM EGTA for 30 minutes prior to incubation in a calcifying solution. After EGTA, an alizarin-red stainable extraneous mineral line is removed, and then following incubation all newly formed mineral is deposited primarily in vesicle-containing areas. Thus, it was possible to enhance significantly the 45Ca uptake into living versus heat inactivated growth plates. Preliminary studies with the EGTA-treated rachitic cartilage also indicate that supplying a matrix vesicle phosphatase substrate such as beta-glycerophosphate (BGP) rather than supplying orthophosphate (Pi) in the incubation mixture leads to more active 45 Ca deposition. We conclude that BGP can be hydrolyzed by vesicle phosphatases to supply Pi for nascent mineral formation, and that our new system promises to be useful in analyzing agents which may stimulate matrix vesicle calcification." @default.
- W2088643445 created "2016-06-24" @default.
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- W2088643445 date "1978-01-01" @default.
- W2088643445 modified "2023-10-18" @default.
- W2088643445 title "A new method to measure 45calcium accumulation by matrix vesicles in slices of rachitic growth plate cartilage" @default.
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- W2088643445 doi "https://doi.org/10.1016/0221-8747(78)90002-4" @default.
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