FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088658300> ?p ?o ?g. }

• W2088658300 endingPage "134" @default.
• W2088658300 startingPage "116" @default.
• W2088658300 abstract "Chromosomal rearrangements, including DNA deletions are involved in carcinogenesis. The deletion (DEL) assay scoring for DNA deletions in the yeast Saccharomyces cerevisiae is able to detect a wide range of carcinogens. Among approximately 60 compounds of known carcinogenic activity, the DEL assay detected 86% correctly whereas the Ames Salmonella assay detected only 30% correctly [R.J. Brennan, R.H. Schiestl, Detecting carcinogens with the yeast DEL assay, Methods Mol. Biol. 262 (2004) 111–124]. Since the DEL assay is highly inducible by DNA double strand breaks, this study examined the utility of the DEL assay for detecting clastogens. Ten model compounds, with varied mechanisms of genotoxicity, were examined for their effect on the frequency of DNA deletions with the DEL assay. The compounds tested were: actinomycin D, camptothecin, methotrexate and 5-fluorodeoxyuridine, which are anticancer agents, noscapine and furosemide are therapeutics, acridine, methyl acrylate and resorcinol are industrial chemicals and diazinon is an insecticide. The in vitro micronucleus assay (IVMN) in CHO cells, a commonly used tool for detection of clastogens, was performed on the same compounds and the results of the two assays were compared. The results of our study show that there is 70% concordance in the presence of metabolic activation (rat liver S9) and 80% concordance in the absence of metabolic activation between the DEL assay and the standard in vitro micronucleus assay. The lack of cytotoxicity observed for four of the ten compounds examined indicates limited diffusion of lipophilic compounds across the yeast cell wall. Thus, the development of a more permeable yeast tester strain is expected to greatly improve concordance of the DEL assay with the IVMN assay. The yeast DEL assay is inexpensive, amenable to automation and requires less expertise to perform than the IVMN. Thus, it has a strong potential as a robust, fast and economical screen for detecting clastogens in vitro." @default.
• W2088658300 created "2016-06-24" @default.
• W2088658300 creator A5007306838 @default.
• W2088658300 creator A5019929521 @default.
• W2088658300 creator A5026683078 @default.
• W2088658300 creator A5047325231 @default.
• W2088658300 creator A5053017129 @default.
• W2088658300 creator A5067326736 @default.
• W2088658300 creator A5076254110 @default.
• W2088658300 date "2005-04-01" @default.
• W2088658300 modified "2023-10-01" @default.
• W2088658300 title "Yeast DEL assay detects clastogens" @default.
• W2088658300 cites W1515564094 @default.
• W2088658300 cites W1962825247 @default.
• W2088658300 cites W1964928576 @default.
• W2088658300 cites W1965199792 @default.
• W2088658300 cites W1968530936 @default.
• W2088658300 cites W1970375612 @default.
• W2088658300 cites W1976120765 @default.
• W2088658300 cites W1982872402 @default.
• W2088658300 cites W1983755397 @default.
• W2088658300 cites W1988562694 @default.
• W2088658300 cites W1989689268 @default.
• W2088658300 cites W1990658681 @default.
• W2088658300 cites W1992880179 @default.
• W2088658300 cites W1992927522 @default.
• W2088658300 cites W1998681909 @default.
• W2088658300 cites W2001291845 @default.
• W2088658300 cites W2008557675 @default.
• W2088658300 cites W2010604387 @default.
• W2088658300 cites W2014215525 @default.
• W2088658300 cites W2014433035 @default.
• W2088658300 cites W2015494582 @default.
• W2088658300 cites W2024264346 @default.
• W2088658300 cites W2024637860 @default.
• W2088658300 cites W2024695320 @default.
• W2088658300 cites W2027348804 @default.
• W2088658300 cites W2029144127 @default.
• W2088658300 cites W2031746340 @default.
• W2088658300 cites W2035210623 @default.
• W2088658300 cites W2038320378 @default.
• W2088658300 cites W2053180080 @default.
• W2088658300 cites W2056204961 @default.
• W2088658300 cites W2058354160 @default.
• W2088658300 cites W2062229879 @default.
• W2088658300 cites W2067475796 @default.
• W2088658300 cites W2068196007 @default.
• W2088658300 cites W2074795894 @default.
• W2088658300 cites W2075963587 @default.
• W2088658300 cites W2082916816 @default.
• W2088658300 cites W2083525898 @default.
• W2088658300 cites W2085411802 @default.
• W2088658300 cites W2086241277 @default.
• W2088658300 cites W2088304084 @default.
• W2088658300 cites W2095377041 @default.
• W2088658300 cites W2098883242 @default.
• W2088658300 cites W2103738438 @default.
• W2088658300 cites W2106019576 @default.
• W2088658300 cites W2127559127 @default.
• W2088658300 cites W2129787793 @default.
• W2088658300 cites W2156618901 @default.
• W2088658300 cites W2167213399 @default.
• W2088658300 cites W2170681445 @default.
• W2088658300 cites W2178784560 @default.
• W2088658300 cites W2774928640 @default.
• W2088658300 cites W4233914881 @default.
• W2088658300 cites W4301761943 @default.
• W2088658300 doi "https://doi.org/10.1016/j.mrgentox.2005.01.005" @default.
• W2088658300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15781217" @default.
• W2088658300 hasPublicationYear "2005" @default.
• W2088658300 type Work @default.
• W2088658300 sameAs 2088658300 @default.
• W2088658300 citedByCount "43" @default.
• W2088658300 countsByYear W20886583002012 @default.
• W2088658300 countsByYear W20886583002013 @default.
• W2088658300 countsByYear W20886583002014 @default.
• W2088658300 countsByYear W20886583002015 @default.
• W2088658300 countsByYear W20886583002017 @default.
• W2088658300 countsByYear W20886583002018 @default.
• W2088658300 countsByYear W20886583002021 @default.
• W2088658300 countsByYear W20886583002022 @default.
• W2088658300 countsByYear W20886583002023 @default.
• W2088658300 crossrefType "journal-article" @default.
• W2088658300 hasAuthorship W2088658300A5007306838 @default.
• W2088658300 hasAuthorship W2088658300A5019929521 @default.
• W2088658300 hasAuthorship W2088658300A5026683078 @default.
• W2088658300 hasAuthorship W2088658300A5047325231 @default.
• W2088658300 hasAuthorship W2088658300A5053017129 @default.
• W2088658300 hasAuthorship W2088658300A5067326736 @default.
• W2088658300 hasAuthorship W2088658300A5076254110 @default.
• W2088658300 hasConcept C114246631 @default.
• W2088658300 hasConcept C117632636 @default.
• W2088658300 hasConcept C141710004 @default.
• W2088658300 hasConcept C153911025 @default.
• W2088658300 hasConcept C178790620 @default.
• W2088658300 hasConcept C185592680 @default.
• W2088658300 hasConcept C2777576037 @default.
• W2088658300 hasConcept C2778959862 @default.

• next