FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088661664> ?p ?o ?g. }

• W2088661664 endingPage "59" @default.
• W2088661664 startingPage "49" @default.
• W2088661664 abstract "Assessment of T cell activation has traditionally been performed by measuring proliferation as a function of 3[H]-thymidine incorporation, or secretion of cytokines from activated peripheral blood mononuclear cells (PBMC) in culture. An alternative method for detection of proliferation at the single cell level utilizes incorporation of bromodeoxyuridine (BrdU), an analog of thymidine, into cellular DNA. After appropriate fixation and permeabilization of the cells, a monoclonal antibody (mAb) against BrdU conjugated with a fluorescent dye is employed to measure by flow cytometry the incorporated BrdU. Here, we report a flow cytometric procedure which can be used for the simultaneous detection of BrdU incorporation, activation markers such as CD69 and CD25, and intracellular cytokines in T cell subsets from activated PBMC. Our observations are consistent with the proposal that cytokine synthesis and cell proliferation occur sequentially in CD4+ T cells stimulated with the superantigen staphylococcal enterotoxin B (SEB). The majority of cells expressing the cytokines IFN-gamma and IL-2 at 48 h appear to have undergone DNA synthesis, however all proliferating cells do not express IFN-gamma or IL-2. The methods presented in this report offer a unique approach for studying simultaneous expression of key cellular activation events in phenotypically resolved lymphocyte populations." @default.
• W2088661664 created "2016-06-24" @default.
• W2088661664 creator A5062858859 @default.
• W2088661664 creator A5067305577 @default.
• W2088661664 date "1997-10-01" @default.
• W2088661664 modified "2023-10-14" @default.
• W2088661664 title "Simultaneous detection of DNA synthesis and cytokine production in staphylococcal enterotoxin B activated CD4+ T lymphocytes by flow cytometry" @default.
• W2088661664 cites W1487652870 @default.
• W2088661664 cites W1490094425 @default.
• W2088661664 cites W1513349945 @default.
• W2088661664 cites W1599108866 @default.
• W2088661664 cites W1829148295 @default.
• W2088661664 cites W1929184634 @default.
• W2088661664 cites W1964731076 @default.
• W2088661664 cites W1993210315 @default.
• W2088661664 cites W1994688157 @default.
• W2088661664 cites W1999002546 @default.
• W2088661664 cites W1999392848 @default.
• W2088661664 cites W2004370735 @default.
• W2088661664 cites W2006215283 @default.
• W2088661664 cites W2028786681 @default.
• W2088661664 cites W2029089280 @default.
• W2088661664 cites W2029836713 @default.
• W2088661664 cites W2031468048 @default.
• W2088661664 cites W2032448671 @default.
• W2088661664 cites W2035625102 @default.
• W2088661664 cites W2035725803 @default.
• W2088661664 cites W2049478058 @default.
• W2088661664 cites W2049998759 @default.
• W2088661664 cites W2052526975 @default.
• W2088661664 cites W2054783092 @default.
• W2088661664 cites W2055669282 @default.
• W2088661664 cites W2059195500 @default.
• W2088661664 cites W2059428912 @default.
• W2088661664 cites W2059490409 @default.
• W2088661664 cites W2062994475 @default.
• W2088661664 cites W2063229043 @default.
• W2088661664 cites W2064596532 @default.
• W2088661664 cites W2067704139 @default.
• W2088661664 cites W2073566510 @default.
• W2088661664 cites W2073897943 @default.
• W2088661664 cites W2086417745 @default.
• W2088661664 cites W2086776723 @default.
• W2088661664 cites W2100117872 @default.
• W2088661664 cites W2112898512 @default.
• W2088661664 cites W2133075460 @default.
• W2088661664 cites W2155782151 @default.
• W2088661664 cites W2158345511 @default.
• W2088661664 cites W2165436746 @default.
• W2088661664 cites W2170548924 @default.
• W2088661664 cites W2171451993 @default.
• W2088661664 cites W2172960056 @default.
• W2088661664 cites W2078007395 @default.
• W2088661664 doi "https://doi.org/10.1016/s0022-1759(97)00127-0" @default.
• W2088661664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9433460" @default.
• W2088661664 hasPublicationYear "1997" @default.
• W2088661664 type Work @default.
• W2088661664 sameAs 2088661664 @default.
• W2088661664 citedByCount "51" @default.
• W2088661664 countsByYear W20886616642012 @default.
• W2088661664 countsByYear W20886616642014 @default.
• W2088661664 crossrefType "journal-article" @default.
• W2088661664 hasAuthorship W2088661664A5062858859 @default.
• W2088661664 hasAuthorship W2088661664A5067305577 @default.
• W2088661664 hasConcept C137061746 @default.
• W2088661664 hasConcept C153911025 @default.
• W2088661664 hasConcept C159654299 @default.
• W2088661664 hasConcept C202751555 @default.
• W2088661664 hasConcept C203014093 @default.
• W2088661664 hasConcept C2776090121 @default.
• W2088661664 hasConcept C2778260397 @default.
• W2088661664 hasConcept C2778690821 @default.
• W2088661664 hasConcept C36333154 @default.
• W2088661664 hasConcept C542903549 @default.
• W2088661664 hasConcept C553184892 @default.
• W2088661664 hasConcept C55493867 @default.
• W2088661664 hasConcept C62112901 @default.
• W2088661664 hasConcept C79484868 @default.
• W2088661664 hasConcept C86803240 @default.
• W2088661664 hasConcept C8891405 @default.
• W2088661664 hasConcept C95444343 @default.
• W2088661664 hasConceptScore W2088661664C137061746 @default.
• W2088661664 hasConceptScore W2088661664C153911025 @default.
• W2088661664 hasConceptScore W2088661664C159654299 @default.
• W2088661664 hasConceptScore W2088661664C202751555 @default.
• W2088661664 hasConceptScore W2088661664C203014093 @default.
• W2088661664 hasConceptScore W2088661664C2776090121 @default.
• W2088661664 hasConceptScore W2088661664C2778260397 @default.
• W2088661664 hasConceptScore W2088661664C2778690821 @default.
• W2088661664 hasConceptScore W2088661664C36333154 @default.
• W2088661664 hasConceptScore W2088661664C542903549 @default.
• W2088661664 hasConceptScore W2088661664C553184892 @default.
• W2088661664 hasConceptScore W2088661664C55493867 @default.
• W2088661664 hasConceptScore W2088661664C62112901 @default.
• W2088661664 hasConceptScore W2088661664C79484868 @default.
• W2088661664 hasConceptScore W2088661664C86803240 @default.
• W2088661664 hasConceptScore W2088661664C8891405 @default.
• W2088661664 hasConceptScore W2088661664C95444343 @default.

• next