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- W2088702936 abstract "For the purpose of studying the transportation of messenger RNA (mRNA) in eucaryotic cells, rat liver nuclei containing rapidly labeled RNA and rapidly labeled proteins, and the unlabeled postmitochondrial fraction (S12 fraction) were incubated with ATP, GTP and an energy-generating system. The transfer of rapidly labeled nRNA and rapidly labeled nuclear proteins from nuclei to polysomes in this reaction mixture was investigated and the following results were obtained: 1. The incorporation of rapidly labeled nRNA and rapidly labeled nuclear proteins into the polysomal fraction increased in parallel for 30 min. This incorporation was confirmed by sucrose density gradient centrifugation of the deoxycholate-treated S12 fraction after incubation. 2. Their incorporation into the polysomal fraction was dependent upon ATP, GTP and an energy-generating system. 3. CsCl buoyant density gradient centrifugation of polysomes prepared from the reconstituted system after incubation, suggested that rapidly labeled nRNA and rapidly labeled nuclear proteins were incorporated into polysomes in the form of a ribonucleoprotein complex, about half of which could be separated from ribosomes by EDTA treatment to the lighter region with a peak at α = 1.41. 4. Sedimentation analysis and the base composition of rapidly labeled nRNA incorporated into polysomes during incubation suggested that a greater part of the radioactivity incorporated into polysomes was messenger-type RNA distributed heterogeneously in the region from 4 to 30 S. 5. From the results on the time course of rapidly labeled nRNA/rapidly labeled nuclear proteins ratios in the polysomal fraction, it was suggested that rapidly labeled nuclear proteins may stay in the polysomes during the translation of newly supplied mRNA. These experimental results may lend support to the hypothesis concerning the transportation of mRNA in eucaryotic cells which proposes that mRNA will be transported from nucleus to cytoplasm in the form of a mRNA-informofer complex and will be incorporated into polysomes to manifest its genetic information." @default.
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- W2088702936 date "1972-01-01" @default.
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- W2088702936 title "Transportation of messenger RNA from nuclei to polysomes in rat liver cells" @default.
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- W2088702936 doi "https://doi.org/10.1016/0005-2787(72)90481-9" @default.
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