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- W2088718592 abstract "Engineering molecular motors with dynamically controllable properties will allow selective perturbation of mechanical processes in vivo. We are developing a set of engineered actomyosin motors in which external signals trigger changes in lever arm geometry and mechanics, with predictable effects on motor properties such as directionality, step size, and processivity. Building on earlier protein engineering studies of directionality determinants [1-2], we previously constructed myosin motors that respond to a change in [Ca++] by reversing their direction of motion along the polarized actin filament [3]. Our designs relied on triggering rigid-to-flexible transitions in chimeric lever arms. We have now extended this work by constructing myosins that respond to optical signals, rather than metal ions. Light is a versatile control signal that can be readily modulated in time and space, and is generally orthogonal to cellular signaling. Using structure-guided protein engineering, we have incorporated photoreceptor domains into the lever arms of chimeric myosin motors. We have generated motors that either speed up, slow down, or switch directions in response to illumination. These genetically encoded motors should be directly deployable inside living cells, and may also be useful for controlling directed transport outside of cellular contexts. [1] Tsiavaliaris G, Fujita-Becker S, Manstein DJ. (2004). Nature, 427, 558-561. [2] Liao J, Elting MW, Delp, SL, Spudich, JA, Bryant Z. (2009). J. Mol. Biol, 392, 862-867. [3] Chen L, Nakamura M, Schindler TD, Bryant Z. Submitted." @default.
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- W2088718592 date "2012-01-01" @default.
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- W2088718592 title "Optical Control of Speed and Directionality in Engineered Myosin Motors" @default.
- W2088718592 doi "https://doi.org/10.1016/j.bpj.2011.11.3096" @default.
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