Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088723535> ?p ?o ?g. }
- W2088723535 endingPage "9767" @default.
- W2088723535 startingPage "9762" @default.
- W2088723535 abstract "Iron-sulfur (Fe-S) clusters serve as cofactors in many proteins that have important redox, catalytic, and regulatory functions. In bacteria, biogenesis of Fe-S clusters is mediated by multiple gene products encoded by the isc and nif operons. In particular, genetic and biochemical studies suggest that IscU, Nfu, and IscA function as scaffold proteins for assembly and delivery of rudimentary Fe-S clusters to target proteins. Here we report the characterization of human Nfu. A combination of biochemical and spectroscopic techniques, including UV-visible absorption and 57Fe Mössbauer spectroscopies, have been used to investigate the ability of purified human Nfu to assemble Fe-S clusters. The results suggest that Nfu can assemble approximately one labile [4Fe-4S] cluster per two Nfu monomers, and support the proposal that Nfu is an alternative scaffold protein for assembly of clusters that are subsequently used for maturation of targeted Fe-S proteins. Analyses of genomic DNA, transcripts, and translation products indicate that alternative splicing of a common pre-mRNA results in synthesis of two Nfu isoforms with distinct subcellular localizations. Isoform I is localized in the mitochondria, whereas isoform II is present in the cytosol and the nucleus. These results, together with previous reports of subcellular distributions of isoforms of human IscS and IscU in mitochondria, cytosol, and nucleus suggest that the Fe-S cluster assembly machineries are compartmentalized in higher eukaryotes." @default.
- W2088723535 created "2016-06-24" @default.
- W2088723535 creator A5017226392 @default.
- W2088723535 creator A5030193476 @default.
- W2088723535 creator A5060588442 @default.
- W2088723535 creator A5068242761 @default.
- W2088723535 date "2003-07-28" @default.
- W2088723535 modified "2023-10-16" @default.
- W2088723535 title "Subcellular compartmentalization of human Nfu, an iron–sulfur cluster scaffold protein, and its ability to assemble a [4Fe–4S] cluster" @default.
- W2088723535 cites W1538399079 @default.
- W2088723535 cites W1550904083 @default.
- W2088723535 cites W1966132636 @default.
- W2088723535 cites W1969490516 @default.
- W2088723535 cites W1972436650 @default.
- W2088723535 cites W1976493565 @default.
- W2088723535 cites W1978004953 @default.
- W2088723535 cites W1985636683 @default.
- W2088723535 cites W1985844569 @default.
- W2088723535 cites W1987421178 @default.
- W2088723535 cites W1987761971 @default.
- W2088723535 cites W1994264299 @default.
- W2088723535 cites W1995312745 @default.
- W2088723535 cites W2000959843 @default.
- W2088723535 cites W2002207630 @default.
- W2088723535 cites W2003418336 @default.
- W2088723535 cites W2006178088 @default.
- W2088723535 cites W2006211612 @default.
- W2088723535 cites W2010961029 @default.
- W2088723535 cites W2013835300 @default.
- W2088723535 cites W2020678097 @default.
- W2088723535 cites W2029990135 @default.
- W2088723535 cites W2031389000 @default.
- W2088723535 cites W2032929186 @default.
- W2088723535 cites W2038950831 @default.
- W2088723535 cites W2039168518 @default.
- W2088723535 cites W2046347557 @default.
- W2088723535 cites W2047906206 @default.
- W2088723535 cites W2051722169 @default.
- W2088723535 cites W2054815069 @default.
- W2088723535 cites W2059027391 @default.
- W2088723535 cites W2059831206 @default.
- W2088723535 cites W2060986410 @default.
- W2088723535 cites W2061040884 @default.
- W2088723535 cites W2061510311 @default.
- W2088723535 cites W2062424661 @default.
- W2088723535 cites W2065894484 @default.
- W2088723535 cites W2072207453 @default.
- W2088723535 cites W2075888213 @default.
- W2088723535 cites W2087921651 @default.
- W2088723535 cites W2089518921 @default.
- W2088723535 cites W2089978314 @default.
- W2088723535 cites W2090079874 @default.
- W2088723535 cites W2091913250 @default.
- W2088723535 cites W2093363808 @default.
- W2088723535 cites W2093853251 @default.
- W2088723535 cites W2094139743 @default.
- W2088723535 cites W2125021980 @default.
- W2088723535 cites W2134503580 @default.
- W2088723535 cites W2146395557 @default.
- W2088723535 cites W2153371171 @default.
- W2088723535 cites W2167063768 @default.
- W2088723535 cites W4300879050 @default.
- W2088723535 doi "https://doi.org/10.1073/pnas.1732541100" @default.
- W2088723535 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/187839" @default.
- W2088723535 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12886008" @default.
- W2088723535 hasPublicationYear "2003" @default.
- W2088723535 type Work @default.
- W2088723535 sameAs 2088723535 @default.
- W2088723535 citedByCount "182" @default.
- W2088723535 countsByYear W20887235352012 @default.
- W2088723535 countsByYear W20887235352013 @default.
- W2088723535 countsByYear W20887235352014 @default.
- W2088723535 countsByYear W20887235352015 @default.
- W2088723535 countsByYear W20887235352016 @default.
- W2088723535 countsByYear W20887235352017 @default.
- W2088723535 countsByYear W20887235352018 @default.
- W2088723535 countsByYear W20887235352019 @default.
- W2088723535 countsByYear W20887235352020 @default.
- W2088723535 countsByYear W20887235352021 @default.
- W2088723535 countsByYear W20887235352022 @default.
- W2088723535 countsByYear W20887235352023 @default.
- W2088723535 crossrefType "journal-article" @default.
- W2088723535 hasAuthorship W2088723535A5017226392 @default.
- W2088723535 hasAuthorship W2088723535A5030193476 @default.
- W2088723535 hasAuthorship W2088723535A5060588442 @default.
- W2088723535 hasAuthorship W2088723535A5068242761 @default.
- W2088723535 hasBestOaLocation W20887235352 @default.
- W2088723535 hasConcept C104317684 @default.
- W2088723535 hasConcept C110455231 @default.
- W2088723535 hasConcept C131934819 @default.
- W2088723535 hasConcept C181199279 @default.
- W2088723535 hasConcept C2776879804 @default.
- W2088723535 hasConcept C2777852564 @default.
- W2088723535 hasConcept C28859421 @default.
- W2088723535 hasConcept C53345823 @default.
- W2088723535 hasConcept C53645450 @default.
- W2088723535 hasConcept C55493867 @default.
- W2088723535 hasConcept C62478195 @default.