FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088731092> ?p ?o ?g. }

• W2088731092 abstract "Possible changes in the size and shape of the glucocorticoid-receptor complex (GRC) following activation remain poorly documented, due to the lability and possible activation of the receptor during the determination of these hydrodynamic parameters. In the present study molybdate was used to stabilize the GRC, thus preventing these uncontrolled transformations. Cytosol prepared from mouse whole brains was incubated for 18 h at 0-2 degrees C with [3H]triamcinolone acetonide (+/- molybdate). Activation was then initiated by incubation at 22 degrees C for variable times and quenched at 0 degree C by adding molybdate. The Stokes radius and sedimentation coefficient of the GRC declined from 77 A and 9.2 S before activation to 58 A and 3.8 S after activation. These measurements remained consistent after recycling GRC between sedimentation and gel filtration procedures and correspond to a 3-fold reduction in the relative molecular mass. The loss and formation of the 297 and 92 kDa species, respectively, after different durations of activation correlated nearly perfectly with increased binding of GRC to DNA-cellulose (DNA-C). The observed size change also correlated well with decreased adsorption to DEAE-cellulose filters (DE-81) and increased adsorption to glass fiber filters (GF/C). The increased adsorption to GF/C may reflect an increase in hydrophobicity which, with extended durations of activation, leads to increased aggregation and reduced binding to DNA-C, but not to a change in adsorption to DE-81. We propose that during activation the 297 kDa form of the GRC splits to form a 92 kDa species that displays an increased affinity for DNA." @default.
• W2088731092 created "2016-06-24" @default.
• W2088731092 creator A5017383178 @default.
• W2088731092 creator A5068077099 @default.
• W2088731092 creator A5075412792 @default.
• W2088731092 date "1984-02-01" @default.
• W2088731092 modified "2023-09-23" @default.
• W2088731092 title "Hydrodynamic and biochemical correlates of the activation of the glucocorticoid-receptor complex" @default.
• W2088731092 cites W1436904535 @default.
• W2088731092 cites W1544928360 @default.
• W2088731092 cites W1567844991 @default.
• W2088731092 cites W1568326379 @default.
• W2088731092 cites W1597345807 @default.
• W2088731092 cites W1601029230 @default.
• W2088731092 cites W1611482599 @default.
• W2088731092 cites W1618332249 @default.
• W2088731092 cites W1775749144 @default.
• W2088731092 cites W178378932 @default.
• W2088731092 cites W1801699123 @default.
• W2088731092 cites W1913466562 @default.
• W2088731092 cites W1964192167 @default.
• W2088731092 cites W1964949703 @default.
• W2088731092 cites W1972307493 @default.
• W2088731092 cites W1978297926 @default.
• W2088731092 cites W1980779622 @default.
• W2088731092 cites W1986797251 @default.
• W2088731092 cites W1989054182 @default.
• W2088731092 cites W2001424604 @default.
• W2088731092 cites W2014139892 @default.
• W2088731092 cites W2018531293 @default.
• W2088731092 cites W2022999461 @default.
• W2088731092 cites W2024304749 @default.
• W2088731092 cites W2033763606 @default.
• W2088731092 cites W2046201653 @default.
• W2088731092 cites W2050586961 @default.
• W2088731092 cites W2057733540 @default.
• W2088731092 cites W2058186197 @default.
• W2088731092 cites W2058638506 @default.
• W2088731092 cites W2065800676 @default.
• W2088731092 cites W2073112355 @default.
• W2088731092 cites W2078142357 @default.
• W2088731092 cites W2083428054 @default.
• W2088731092 cites W2093284364 @default.
• W2088731092 cites W2095495834 @default.
• W2088731092 cites W2208707498 @default.
• W2088731092 cites W2211434907 @default.
• W2088731092 cites W2342073136 @default.
• W2088731092 cites W2345032033 @default.
• W2088731092 cites W2494862425 @default.
• W2088731092 cites W966356403 @default.
• W2088731092 doi "https://doi.org/10.1016/0022-4731(84)90122-5" @default.
• W2088731092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6708537" @default.
• W2088731092 hasPublicationYear "1984" @default.
• W2088731092 type Work @default.
• W2088731092 sameAs 2088731092 @default.
• W2088731092 citedByCount "27" @default.
• W2088731092 crossrefType "journal-article" @default.
• W2088731092 hasAuthorship W2088731092A5017383178 @default.
• W2088731092 hasAuthorship W2088731092A5068077099 @default.
• W2088731092 hasAuthorship W2088731092A5075412792 @default.
• W2088731092 hasConcept C12554922 @default.
• W2088731092 hasConcept C136543978 @default.
• W2088731092 hasConcept C1388644 @default.
• W2088731092 hasConcept C149997192 @default.
• W2088731092 hasConcept C150394285 @default.
• W2088731092 hasConcept C170493617 @default.
• W2088731092 hasConcept C178790620 @default.
• W2088731092 hasConcept C179104552 @default.
• W2088731092 hasConcept C181199279 @default.
• W2088731092 hasConcept C185592680 @default.
• W2088731092 hasConcept C2775981058 @default.
• W2088731092 hasConcept C43617362 @default.
• W2088731092 hasConcept C552990157 @default.
• W2088731092 hasConcept C55493867 @default.
• W2088731092 hasConcept C59493245 @default.
• W2088731092 hasConcept C86803240 @default.
• W2088731092 hasConcept C98539663 @default.
• W2088731092 hasConceptScore W2088731092C12554922 @default.
• W2088731092 hasConceptScore W2088731092C136543978 @default.
• W2088731092 hasConceptScore W2088731092C1388644 @default.
• W2088731092 hasConceptScore W2088731092C149997192 @default.
• W2088731092 hasConceptScore W2088731092C150394285 @default.
• W2088731092 hasConceptScore W2088731092C170493617 @default.
• W2088731092 hasConceptScore W2088731092C178790620 @default.
• W2088731092 hasConceptScore W2088731092C179104552 @default.
• W2088731092 hasConceptScore W2088731092C181199279 @default.
• W2088731092 hasConceptScore W2088731092C185592680 @default.
• W2088731092 hasConceptScore W2088731092C2775981058 @default.
• W2088731092 hasConceptScore W2088731092C43617362 @default.
• W2088731092 hasConceptScore W2088731092C552990157 @default.
• W2088731092 hasConceptScore W2088731092C55493867 @default.
• W2088731092 hasConceptScore W2088731092C59493245 @default.
• W2088731092 hasConceptScore W2088731092C86803240 @default.
• W2088731092 hasConceptScore W2088731092C98539663 @default.
• W2088731092 hasLocation W20887310921 @default.
• W2088731092 hasLocation W20887310922 @default.
• W2088731092 hasOpenAccess W2088731092 @default.
• W2088731092 hasPrimaryLocation W20887310921 @default.
• W2088731092 hasRelatedWork W1629193539 @default.
• W2088731092 hasRelatedWork W1979608106 @default.

• next