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- W2088756875 abstract "The phase diagram of a protein–water system is described with a simple model with parameters for the interaction between the protein molecules in the crystal and in the solution. For a certain range of these parameters the phase diagram shows a metastable liquid–liquid immiscibility region. It is shown that this region corresponds to the “crystallization slot” for growing crystals, as proposed by George and Wilson [Acta Crystallogr. D 50 (1994) 361]. Nucleation in this region proceeds in two steps. First small liquid droplets with a high protein concentration are formed; then small crystalline nuclei grow inside these droplets. In the crystallization slot crystals are covered by a thin liquid film with a high protein concentration. We discuss NMR experiments on lysozyme, which show that nucleation is a transient process with an induction time." @default.
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- W2088756875 date "1999-01-01" @default.
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- W2088756875 title "Understanding protein crystallization on the basis of the phase diagram" @default.
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- W2088756875 doi "https://doi.org/10.1016/s0022-0248(98)00831-8" @default.
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