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- W2088775306 abstract "Lactobacillus acidophilus CRL 640 grown at the optimal temperature of 37°C (M37) appeared more sensitive to freeze-thawing than when it was grown at 25°C (M25). In the first case, 87% of the cells died, in contrast to 33% for cells grown at 25°C. All the surviving M37 cells showed sensitivity to NaCl. However, among the surviving M25 cells, only 85% were sensitive to NaCl. The rest of the cells were considered uninjured. Freeze-thawing in cells grown at 25°C showed a liberation of nucleic acids and proteins. However, the leakage was higher in M37 cells after freeze–thawing. The greater fraction of damaged cells were observed in M25 culture after freeze–thawing. A relative increase of 81% in cardiolipid (CL), with respect to total phospholipids and 72% triglycosyldiglyceride (TGDG) with respect to the total glycolipids was observed in M37. In addition, a decrease of palmitoyl (C16:0), oleoyl (C18:0) fatty acids at CL, phosphatidylglycerol (PG), and diglicosyldiglyceride (DGDG) fractions and the increase of C19 cyc and C18:0, 10-OH fatty acids in neutral lipid, and CL fractions was also apparent. In M25 cells, the concentration of DGDG and PG was higher than in M37 cells. The difference in cryotolerance between the frozen cultures emphasizes the importance of selecting appropriate conditions of growth of microorganisms for use as dietary adjuncts." @default.
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- W2088775306 date "2001-04-01" @default.
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- W2088775306 title "Effect of Lipid Composition on the Stability of Cellular Membranes during Freeze–Thawing of Lactobacillus acidophilus Grown at Different Temperatures" @default.
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- W2088775306 doi "https://doi.org/10.1006/abbi.2001.2274" @default.
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