FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088819616> ?p ?o ?g. }

• W2088819616 endingPage "1896" @default.
• W2088819616 startingPage "1883" @default.
• W2088819616 abstract "One strategy to improve therapies in advanced prostate cancer (PC) involves targeting genes that are activated by androgen withdrawal to delay the emergence of the androgen-independent (AI) phenotype. Heat shock protein 27 (Hsp27) expression becomes highly upregulated in PC cells after androgen withdrawal or chemotherapy, in which it functions as a cytoprotective chaperone to confer broad-spectrum treatment resistance. The purpose of this study is to elucidate anti-apoptotic pathways regulated by Hsp27 that are activated during PC progression. Using two-hybrid experiment, we found that Hsp27 was having a major role in the protein translational initiation process. Furthermore, using complementary DNA (cDNA) microarray analysis, 4E binding protein 1 was identified as being proportionately and highly regulated by Hsp27. These data led us to analyze the protein synthesis initiation pathway, which is a prerequisite for cell growth and proliferation. Using northern and western blot analysis, we found that Hsp27 downregulation decreased eukaryotic translation initiation factor 4E (eIF4E) expression at the protein, but not mRNA, level. The cytoprotection afforded by Hsp27 overexpression was attenuated by eIF4E knockdown using specific eIF4E short interfering RNA (siRNA). Co-immunoprecipitation and co-immunofluorescence confirmed that Hsp27 colocalizes and interacts directly with eIF4E. Hsp27-eIF4E interaction decreases eIF4E ubiquitination and proteasomal degradation. By chaperoning eIF4E, Hsp27 seems to protect the protein synthesis initiation process to enhance cell survival during cell stress induced by castration or chemotherapy. Forced overexpression of eIF4E induces resistance to androgen-withdrawal and paclitaxel treatment in the prostate LNCaP cells in vitro. These findings identify Hsp27 as a modulator of eIF4E and establish a potential mechanism for the eIF4E-regulated apoptosis after androgen ablation and chemotherapy. Targeting Hsp27-eIF4E interaction may serve as a therapeutic target in advanced PC." @default.
• W2088819616 created "2016-06-24" @default.
• W2088819616 creator A5009043218 @default.
• W2088819616 creator A5011164078 @default.
• W2088819616 creator A5013015930 @default.
• W2088819616 creator A5034110828 @default.
• W2088819616 creator A5038321597 @default.
• W2088819616 creator A5046067066 @default.
• W2088819616 creator A5062125605 @default.
• W2088819616 creator A5064510604 @default.
• W2088819616 creator A5066948009 @default.
• W2088819616 creator A5070423831 @default.
• W2088819616 creator A5074609153 @default.
• W2088819616 creator A5076002944 @default.
• W2088819616 creator A5083394465 @default.
• W2088819616 creator A5089480920 @default.
• W2088819616 date "2010-01-18" @default.
• W2088819616 modified "2023-10-17" @default.
• W2088819616 title "Heat shock protein 27 confers resistance to androgen ablation and chemotherapy in prostate cancer cells through eIF4E" @default.
• W2088819616 cites W1968330747 @default.
• W2088819616 cites W1980234430 @default.
• W2088819616 cites W1987499203 @default.
• W2088819616 cites W1996755153 @default.
• W2088819616 cites W2001443196 @default.
• W2088819616 cites W2003278673 @default.
• W2088819616 cites W2005056727 @default.
• W2088819616 cites W2005202590 @default.
• W2088819616 cites W2011866867 @default.
• W2088819616 cites W2011961482 @default.
• W2088819616 cites W2025444969 @default.
• W2088819616 cites W2034404031 @default.
• W2088819616 cites W2034710772 @default.
• W2088819616 cites W2047104027 @default.
• W2088819616 cites W2049490068 @default.
• W2088819616 cites W2057623594 @default.
• W2088819616 cites W2062099953 @default.
• W2088819616 cites W2065825082 @default.
• W2088819616 cites W2072189442 @default.
• W2088819616 cites W2074399578 @default.
• W2088819616 cites W2075511135 @default.
• W2088819616 cites W2077551355 @default.
• W2088819616 cites W2080439368 @default.
• W2088819616 cites W2082067798 @default.
• W2088819616 cites W2085864574 @default.
• W2088819616 cites W2089265649 @default.
• W2088819616 cites W2096088010 @default.
• W2088819616 cites W2106113955 @default.
• W2088819616 cites W2106626264 @default.
• W2088819616 cites W2114788766 @default.
• W2088819616 cites W2114901348 @default.
• W2088819616 cites W2119036448 @default.
• W2088819616 cites W2120292596 @default.
• W2088819616 cites W2123671636 @default.
• W2088819616 cites W2141278758 @default.
• W2088819616 cites W2143609278 @default.
• W2088819616 cites W2148915086 @default.
• W2088819616 cites W2154305555 @default.
• W2088819616 cites W2159554844 @default.
• W2088819616 cites W2170677834 @default.
• W2088819616 cites W2412431453 @default.
• W2088819616 cites W4236776658 @default.
• W2088819616 doi "https://doi.org/10.1038/onc.2009.479" @default.
• W2088819616 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20101233" @default.
• W2088819616 hasPublicationYear "2010" @default.
• W2088819616 type Work @default.
• W2088819616 sameAs 2088819616 @default.
• W2088819616 citedByCount "120" @default.
• W2088819616 countsByYear W20888196162012 @default.
• W2088819616 countsByYear W20888196162013 @default.
• W2088819616 countsByYear W20888196162014 @default.
• W2088819616 countsByYear W20888196162015 @default.
• W2088819616 countsByYear W20888196162016 @default.
• W2088819616 countsByYear W20888196162017 @default.
• W2088819616 countsByYear W20888196162018 @default.
• W2088819616 countsByYear W20888196162019 @default.
• W2088819616 countsByYear W20888196162020 @default.
• W2088819616 countsByYear W20888196162021 @default.
• W2088819616 countsByYear W20888196162022 @default.
• W2088819616 countsByYear W20888196162023 @default.
• W2088819616 crossrefType "journal-article" @default.
• W2088819616 hasAuthorship W2088819616A5009043218 @default.
• W2088819616 hasAuthorship W2088819616A5011164078 @default.
• W2088819616 hasAuthorship W2088819616A5013015930 @default.
• W2088819616 hasAuthorship W2088819616A5034110828 @default.
• W2088819616 hasAuthorship W2088819616A5038321597 @default.
• W2088819616 hasAuthorship W2088819616A5046067066 @default.
• W2088819616 hasAuthorship W2088819616A5062125605 @default.
• W2088819616 hasAuthorship W2088819616A5064510604 @default.
• W2088819616 hasAuthorship W2088819616A5066948009 @default.
• W2088819616 hasAuthorship W2088819616A5070423831 @default.
• W2088819616 hasAuthorship W2088819616A5074609153 @default.
• W2088819616 hasAuthorship W2088819616A5076002944 @default.
• W2088819616 hasAuthorship W2088819616A5083394465 @default.
• W2088819616 hasAuthorship W2088819616A5089480920 @default.
• W2088819616 hasBestOaLocation W20888196161 @default.
• W2088819616 hasConcept C104317684 @default.
• W2088819616 hasConcept C105580179 @default.
• W2088819616 hasConcept C121608353 @default.

• next