Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088879103> ?p ?o ?g. }
- W2088879103 endingPage "885" @default.
- W2088879103 startingPage "879" @default.
- W2088879103 abstract "A novel fluorescent protein termed hmGFP homologous to the green fluorescent protein (GFP) from Aequorea victoria was cloned from the tentacles of sea anemone Heteractis magnifica by EST sequencing and analysis of cDNA library and followed by using RT-PCR. The sequence analysis suggested that the chromophore, consensus amino acids, and secondary structure of 11 beta-strands of hmGFP were similar to those of GFP from other species. The recombinant hmGFP protein with high purity was obtained by the fusion expression of pETTRX-hmGFP in Escherichia coli and subsequent purification. The pH sensitivity and fluorescence spectroscopy of recombinant hmGFP were characterized. The excitation spectrum of recombinant hmGFP has a rather wide major peak with a maximum at 490 nm and a shoulder at 420 nm, and its emission spectrum at 510 nm. The expression of hmGFP and the chimera IPL through hmGFP in CHO cells has shown that the fusion protein IPL through hmGFP has retained the normal membrane targeting of the IPL from Dasyatis akajei, as well as maintaining fluorescent properties similar to those of native hmGFP, suggesting a promising prospect of the application in biotechnology research for the new protein." @default.
- W2088879103 created "2016-06-24" @default.
- W2088879103 creator A5000449640 @default.
- W2088879103 creator A5003738856 @default.
- W2088879103 creator A5010972070 @default.
- W2088879103 creator A5012291182 @default.
- W2088879103 creator A5023771407 @default.
- W2088879103 creator A5051084283 @default.
- W2088879103 creator A5053885203 @default.
- W2088879103 creator A5055382254 @default.
- W2088879103 creator A5065814437 @default.
- W2088879103 creator A5084442984 @default.
- W2088879103 creator A5084685500 @default.
- W2088879103 creator A5086520105 @default.
- W2088879103 creator A5086680659 @default.
- W2088879103 date "2003-02-01" @default.
- W2088879103 modified "2023-10-17" @default.
- W2088879103 title "A naturally enhanced green fluorescent protein from magnificent sea anemone (Heteractis magnifica) and its functional analysis" @default.
- W2088879103 cites W127850330 @default.
- W2088879103 cites W1490724066 @default.
- W2088879103 cites W1522693579 @default.
- W2088879103 cites W1544900705 @default.
- W2088879103 cites W1789943808 @default.
- W2088879103 cites W1964359053 @default.
- W2088879103 cites W1972974751 @default.
- W2088879103 cites W1990912317 @default.
- W2088879103 cites W2001741929 @default.
- W2088879103 cites W2002360833 @default.
- W2088879103 cites W2006685379 @default.
- W2088879103 cites W2014293907 @default.
- W2088879103 cites W2016441216 @default.
- W2088879103 cites W2018361147 @default.
- W2088879103 cites W2029309165 @default.
- W2088879103 cites W2030695915 @default.
- W2088879103 cites W2032579724 @default.
- W2088879103 cites W2035843420 @default.
- W2088879103 cites W2038756774 @default.
- W2088879103 cites W2042352204 @default.
- W2088879103 cites W2047917279 @default.
- W2088879103 cites W2055649671 @default.
- W2088879103 cites W2061068107 @default.
- W2088879103 cites W2071496486 @default.
- W2088879103 cites W2083592023 @default.
- W2088879103 cites W2127068865 @default.
- W2088879103 cites W2134190459 @default.
- W2088879103 cites W2134907232 @default.
- W2088879103 cites W2138468193 @default.
- W2088879103 cites W2158714788 @default.
- W2088879103 cites W2161996750 @default.
- W2088879103 cites W2167353442 @default.
- W2088879103 cites W4233808990 @default.
- W2088879103 cites W4236962085 @default.
- W2088879103 cites W4249406446 @default.
- W2088879103 doi "https://doi.org/10.1016/s0006-291x(03)00019-6" @default.
- W2088879103 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12589794" @default.
- W2088879103 hasPublicationYear "2003" @default.
- W2088879103 type Work @default.
- W2088879103 sameAs 2088879103 @default.
- W2088879103 citedByCount "12" @default.
- W2088879103 countsByYear W20888791032013 @default.
- W2088879103 countsByYear W20888791032015 @default.
- W2088879103 countsByYear W20888791032016 @default.
- W2088879103 countsByYear W20888791032017 @default.
- W2088879103 countsByYear W20888791032019 @default.
- W2088879103 crossrefType "journal-article" @default.
- W2088879103 hasAuthorship W2088879103A5000449640 @default.
- W2088879103 hasAuthorship W2088879103A5003738856 @default.
- W2088879103 hasAuthorship W2088879103A5010972070 @default.
- W2088879103 hasAuthorship W2088879103A5012291182 @default.
- W2088879103 hasAuthorship W2088879103A5023771407 @default.
- W2088879103 hasAuthorship W2088879103A5051084283 @default.
- W2088879103 hasAuthorship W2088879103A5053885203 @default.
- W2088879103 hasAuthorship W2088879103A5055382254 @default.
- W2088879103 hasAuthorship W2088879103A5065814437 @default.
- W2088879103 hasAuthorship W2088879103A5084442984 @default.
- W2088879103 hasAuthorship W2088879103A5084685500 @default.
- W2088879103 hasAuthorship W2088879103A5086520105 @default.
- W2088879103 hasAuthorship W2088879103A5086680659 @default.
- W2088879103 hasConcept C104317684 @default.
- W2088879103 hasConcept C121332964 @default.
- W2088879103 hasConcept C123894998 @default.
- W2088879103 hasConcept C12554922 @default.
- W2088879103 hasConcept C142613039 @default.
- W2088879103 hasConcept C153911025 @default.
- W2088879103 hasConcept C185592680 @default.
- W2088879103 hasConcept C187882448 @default.
- W2088879103 hasConcept C2777105759 @default.
- W2088879103 hasConcept C2778089062 @default.
- W2088879103 hasConcept C40767141 @default.
- W2088879103 hasConcept C547475151 @default.
- W2088879103 hasConcept C55493867 @default.
- W2088879103 hasConcept C59822182 @default.
- W2088879103 hasConcept C62520636 @default.
- W2088879103 hasConcept C86803240 @default.
- W2088879103 hasConcept C91881484 @default.
- W2088879103 hasConceptScore W2088879103C104317684 @default.
- W2088879103 hasConceptScore W2088879103C121332964 @default.
- W2088879103 hasConceptScore W2088879103C123894998 @default.