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- W2088891507 abstract "A sequence of three tandem repeats of a 13-mer in the replication origin (oriC) of E. coli is the highly conserved site of opening of the duplex for initiation of DNA synthesis. A protein that binds this sequence has been discovered in E. coli and purified to homogeneity. This novel 33 kd polypeptide behaves as a dimer. Binding to the 13-mers is specific and limited to this region. At a ratio of 10-20 monomers per oriC plasmid, the binding blocks initiation by preventing the opening of the 13-mer region by dnaA protein. Once the 13-mers are opened by dnaA protein action, the 33 kd protein is without effect on the subsequent stages of replication. The specificity of binding and profound inhibitory effect suggest a regulatory role for this protein at an early stage of chromosome initiation." @default.
- W2088891507 created "2016-06-24" @default.
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- W2088891507 date "1990-10-01" @default.
- W2088891507 modified "2023-09-24" @default.
- W2088891507 title "A novel protein binds a key origin sequence to block replication of an E. coli minichromosome" @default.
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- W2088891507 doi "https://doi.org/10.1016/0092-8674(90)90165-b" @default.
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