FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088892171> ?p ?o ?g. }

• W2088892171 endingPage "599" @default.
• W2088892171 startingPage "587" @default.
• W2088892171 abstract "The enzyme cholesterol lecithin acyl transferase (LCAT) shares the Ser/Asp-Glu/His triad with lipases, esterases and proteases, but the low level of sequence homology between LCAT and these enzymes did not allow for the LCAT fold to be identified yet. We, therefore, relied upon structural homology calculations using threading methods based on alignment of the sequence against a library of solved three-dimensional protein structures, for prediction of the LCAT fold. We propose that LCAT, like lipases, belongs to the alpha/beta hydrolase fold family, and that the central domain of LCAT consists of seven conserved parallel beta-strands connected by four alpha-helices and separated by loops. We used the conserved features of this protein fold for the prediction of functional domains in LCAT, and carried out site-directed mutagenesis for the localization of the active site residues. The wild-type enzyme and mutants were expressed in Cos-1 cells. LCAT mass was measured by ELISA, and enzymatic activity was measured on recombinant HDL, on LDL and on a monomeric substrate. We identified D345 and H377 as the catalytic residues of LCAT, together with F103 and L182 as the oxyanion hole residues. In analogy with lipases, we further propose that a potential lid domain at residues 50-74 of LCAT might be involved in the enzyme-substrate interaction. Molecular modeling of human LCAT was carried out using human pancreatic and Candida antarctica lipases as templates. The three-dimensional model proposed here is compatible with the position of natural mutants for either LCAT deficiency or Fish-eye disease. It enables moreover prediction of the LCAT domains involved in the interaction with the phospholipid and cholesterol substrates." @default.
• W2088892171 created "2016-06-24" @default.
• W2088892171 creator A5000046652 @default.
• W2088892171 creator A5017662636 @default.
• W2088892171 creator A5020317371 @default.
• W2088892171 creator A5035904692 @default.
• W2088892171 creator A5054960368 @default.
• W2088892171 creator A5054994879 @default.
• W2088892171 creator A5055148836 @default.
• W2088892171 creator A5057513592 @default.
• W2088892171 creator A5072239633 @default.
• W2088892171 creator A5075683165 @default.
• W2088892171 creator A5075879769 @default.
• W2088892171 creator A5090528097 @default.
• W2088892171 date "1998-03-01" @default.
• W2088892171 modified "2023-10-13" @default.
• W2088892171 title "A proposed architecture for lecithin cholesterol acyl transferase (LCAT): Identification of the catalytic triad and molecular modeling" @default.
• W2088892171 cites W1480395470 @default.
• W2088892171 cites W1500853612 @default.
• W2088892171 cites W1603442691 @default.
• W2088892171 cites W1614929635 @default.
• W2088892171 cites W1927538892 @default.
• W2088892171 cites W1964106762 @default.
• W2088892171 cites W1964636290 @default.
• W2088892171 cites W1969172212 @default.
• W2088892171 cites W1969743161 @default.
• W2088892171 cites W1969853805 @default.
• W2088892171 cites W197238901 @default.
• W2088892171 cites W1974603745 @default.
• W2088892171 cites W1975002518 @default.
• W2088892171 cites W1985818354 @default.
• W2088892171 cites W1995773911 @default.
• W2088892171 cites W2012176370 @default.
• W2088892171 cites W2022520842 @default.
• W2088892171 cites W2026008765 @default.
• W2088892171 cites W2033496784 @default.
• W2088892171 cites W2046429206 @default.
• W2088892171 cites W2047376475 @default.
• W2088892171 cites W2050125414 @default.
• W2088892171 cites W2053721311 @default.
• W2088892171 cites W2055043387 @default.
• W2088892171 cites W2056785733 @default.
• W2088892171 cites W2073636691 @default.
• W2088892171 cites W2080167309 @default.
• W2088892171 cites W2082015820 @default.
• W2088892171 cites W2084568049 @default.
• W2088892171 cites W2085789739 @default.
• W2088892171 cites W2094031081 @default.
• W2088892171 cites W2095294218 @default.
• W2088892171 cites W2099943177 @default.
• W2088892171 cites W2101870257 @default.
• W2088892171 cites W2113403117 @default.
• W2088892171 cites W2113504642 @default.
• W2088892171 cites W2122384861 @default.
• W2088892171 cites W2138361590 @default.
• W2088892171 cites W2158888718 @default.
• W2088892171 cites W2161615797 @default.
• W2088892171 cites W2164320858 @default.
• W2088892171 cites W2166946451 @default.
• W2088892171 cites W2167085937 @default.
• W2088892171 cites W2171335714 @default.
• W2088892171 cites W2182551275 @default.
• W2088892171 cites W2184111207 @default.
• W2088892171 cites W2402903841 @default.
• W2088892171 cites W2414307244 @default.
• W2088892171 cites W4205995961 @default.
• W2088892171 doi "https://doi.org/10.1002/pro.5560070307" @default.
• W2088892171 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2143955" @default.
• W2088892171 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9541390" @default.
• W2088892171 hasPublicationYear "1998" @default.
• W2088892171 type Work @default.
• W2088892171 sameAs 2088892171 @default.
• W2088892171 citedByCount "87" @default.
• W2088892171 countsByYear W20888921712012 @default.
• W2088892171 countsByYear W20888921712013 @default.
• W2088892171 countsByYear W20888921712014 @default.
• W2088892171 countsByYear W20888921712015 @default.
• W2088892171 countsByYear W20888921712016 @default.
• W2088892171 countsByYear W20888921712017 @default.
• W2088892171 countsByYear W20888921712018 @default.
• W2088892171 countsByYear W20888921712020 @default.
• W2088892171 countsByYear W20888921712021 @default.
• W2088892171 countsByYear W20888921712022 @default.
• W2088892171 crossrefType "journal-article" @default.
• W2088892171 hasAuthorship W2088892171A5000046652 @default.
• W2088892171 hasAuthorship W2088892171A5017662636 @default.
• W2088892171 hasAuthorship W2088892171A5020317371 @default.
• W2088892171 hasAuthorship W2088892171A5035904692 @default.
• W2088892171 hasAuthorship W2088892171A5054960368 @default.
• W2088892171 hasAuthorship W2088892171A5054994879 @default.
• W2088892171 hasAuthorship W2088892171A5055148836 @default.
• W2088892171 hasAuthorship W2088892171A5057513592 @default.
• W2088892171 hasAuthorship W2088892171A5072239633 @default.
• W2088892171 hasAuthorship W2088892171A5075683165 @default.
• W2088892171 hasAuthorship W2088892171A5075879769 @default.
• W2088892171 hasAuthorship W2088892171A5090528097 @default.
• W2088892171 hasBestOaLocation W20888921712 @default.
• W2088892171 hasConcept C145770059 @default.

• next