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- W2088892264 abstract "Sections of pancreatic islets from C57BL/6J mice aged 3, 14, and 24 months, consisting of islets derived from the dorsal primordium (DPI) and from the ventral primordium (VPI), were immunostained using the peroxidase-antiperoxidase (PAP) procedure for localization of glucagon (A cells) and somatostatin (D cells). The density (A or D cell area/islet area) of immunopositive cells were determined using computer-assisted image analysis. The density of A cells was significantly less in VPI of 14- and 24-month-old mice compared to 3-month-old mice. The density of A cells in 24 month DPI was less than 3 month DPI but no different from 14 month DPI. The mean area (μm2) of A cells (only in DPI) was significantly less at 24 months compared to the 3 and 14 month groups. There were no differences in somatostatin staining when comparing the three age groups, although at all ages the density of D cells was always greater in the DPI. In conclusion, the major difference between the young and older mice was a deficiency of glucagonstained cells in older mice. These results might be important in explaining improved glucose tolerance in aged C57BL/6J mice." @default.
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- W2088892264 date "1990-09-01" @default.
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- W2088892264 title "Age-related immunohistochemical studies of A and D cells in pancreatic islets of C57BL/6J mice" @default.
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- W2088892264 doi "https://doi.org/10.1002/ar.1092280109" @default.
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