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• W2088892613 abstract "For DNA single molecule sequencing, the complete detection of all dye-labeled monomers which are cleaved off during the sequencing reaction is an essential requirement. In this work we address the feasibility of single molecule detection in microstructures with a confocal multi element set-up. We present statistical data on single molecule recognition and explain a refined data evaluation technique for single molecule burst analysis. From these data the signal-to-noise ratio in microstructures is evaluated as well as the overall detection efficiency. So far, detection efficiencies of single molecule events of up to 60% have been shown in microstructures." @default.
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• W2088892613 date "2001-04-01" @default.
• W2088892613 modified "2023-09-23" @default.
• W2088892613 title "Highly efficient single molecule detection in microstructures" @default.
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• W2088892613 doi "https://doi.org/10.1016/s0168-1656(00)00415-6" @default.
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