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• W2088896879 abstract "<i>Objectives:</i> To construct a recombinant baculovirus expressing the fiber knob domain of human adenovirus type 2 modified by the insertion of a foreign peptide, purify this protein after its production in insect cells, and to test its properties. <i>Methods:</i> Recombinant baculoviruses expressing the fiber knob were produced in <i>Sf</i>9 cells. The recombinant fiber knob was recovered from culture supernatants of infected cells and purified by a combination of Ni-NTA and ion-exchange chromatography. <i>Results:</i> Fiber knob was recovered from the culture media as a soluble protein. In the system used, the fiber knob is expressed fused with the V5 epitope and a histidine tag, which allowed purification by Ni-NTA chromatography. The protein was further purified by ion-exchange chromatography. We show that the recombinant fiber knob produced, with 31 extra amino acids in the C-terminus, can oligomerize and bind to the adenovirus receptor CAR, as it can block the infection of a recombinant type 5 adenovirus. <i>Conclusions:</i> The modified form of the fiber knob, produced in insect cells and purified by Ni-NTA and ion-exchange chromatography, retains the properties of oligomerization and binding to the fiber natural receptor, CAR. This construct has the potential to be a new adjuvant." @default.
• W2088896879 created "2016-06-24" @default.
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• W2088896879 date "2008-01-01" @default.
• W2088896879 modified "2023-09-27" @default.
• W2088896879 title "Expression and Purification of a Recombinant Adenovirus Fiber Knob in a Baculovirus System" @default.
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• W2088896879 doi "https://doi.org/10.1159/000151532" @default.
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