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- W2088897244 abstract "No AccessJournal of UrologyInvestigative Urology1 Dec 2010Receptor Mediated Agglutination of Human Spermatozoa by Spermagglutinating Factor Isolated From Staphylococcus aureus Siftjit Kaur, Vijay Prabha, and Abha Sarwal Siftjit KaurSiftjit Kaur More articles by this author , Vijay PrabhaVijay Prabha More articles by this author , and Abha SarwalAbha Sarwal More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.07.031AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We examined spermagglutinating factor isolated from Staphylococcus aureus for evidence of receptor mediated agglutination of human spermatozoa. Materials and Methods: Binding to spermatozoa by spermagglutinating factor isolated from S. aureus with a high degree of specificity indicates receptor-ligand interaction. To examine this interaction we isolated and purified the ligand and the receptor. To assess receptor mediated agglutination of spermatozoa further we blocked spermagglutination induced by spermagglutinating factor in the presence of receptor. Results: Spermagglutinating factor induced spermagglutination was competitively inhibited by adding purified receptor, indicating that sperm agglutinating factor isolated from S. aureus attaches to specific receptors on human spermatozoa. The spermagglutinating factor receptor was a protein with a molecular weight of approximately 57 kDa. Spermagglutinating factor induced spermagglutination and at higher concentrations had a spermicidal effect, which was inhibited by introducing the receptor. As observed on scanning electron microscopy studies, incubating spermatozoa with spermagglutinating factor showed profound morphological alterations. However, spermatozoa with normal morphology were noted when incubated with spermagglutinating factor in the presence of receptor, indicating that morphological alterations may account for spermatozoa agglutination by spermagglutinating factor. Conclusions: Results suggest that spermagglutinating factor isolated from S. aureus may bind specifically to sperm surface receptor sites before causing spermagglutination. References 1 : The effect of bacterial infection on the quality of human's spermatozoa. Iranian J Publ Health2001; 30: 119. Google Scholar 2 : Infections of the male reproductive tract and infertility: a selected review. J Androl1980; 3: 121. Google Scholar 3 : Asymptomatic bacteriospermia in fertile and infertile men. Fertil Steril1981; 36: 88. Google Scholar 4 : Virulence characteristics of male genital tract Escherichia coli isolated from semen of suspected infertile men. Andrologia1991; 23: 387. 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Link, Google Scholar 12 : Detection of bacteria from semen of infertile males and their seminal parameters. Chin J Androl1996; 10: 196. Google Scholar 13 : Microbial isolates and HIV infection in couples attending fertility clinics in Sagamu, Nigeria. J Obstet Gynecol2005; 25: 685. Google Scholar 14 : Isolation of sperm-agglutinating factor from Staphylococcus aureus isolated from a woman with unexplained infertility. Fertil Steril2005; 84: 1539. Google Scholar 15 WHO Laboratory Manual for the Examination of Human Semen and Semen-Cervical Mucus Interaction: World Health Organization. Cambridge, United Kingdom: Cambridge University Press1999. Google Scholar 16 : Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970; 227: 680. Google Scholar 17 : Supravital staining of human spermatozoa. Fertil Steril1977; 28: 1257. Google Scholar 18 : Scanning electron microscopy of human, monkey and rabbit spermatozoa. Fertil Steril1973; 24: 776. Google Scholar 19 : Adherence of Escherichia coli to sperm: a mannose mediated phenomenon leading to agglutination of sperm and E. coli. Fertil Steril1993; 60: 154. Google Scholar 20 : Interference of human sperm motility by Escherichia coli. Fertil Steril1971; 22: 281. Google Scholar 21 : Isolation of a spermatozoal immobilization factor from Escherichia coli culture filtrates. Fertil Steril1977; 28: 182. Google Scholar © 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 184Issue 6December 2010Page: 2586-2590 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.KeywordsinfertilitymalespermatozoaStaphylococcus aureusagglutinationtestisMetricsAuthor Information Siftjit Kaur More articles by this author Vijay Prabha More articles by this author Abha Sarwal More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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- W2088897244 title "Receptor Mediated Agglutination of Human Spermatozoa by Spermagglutinating Factor Isolated From Staphylococcus aureus" @default.
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