FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088906085> ?p ?o ?g. }

• W2088906085 abstract "The role of the spectrin-based membrane skeleton in cell fusion was studied by following the condition-dependent diameter versus time expansion signature of the fusion zone in electrofused pairs of erythrocyte ghost membranes. Previous work showed that the presence of the dielectrophoresis-inducing alternating electric field, which is used to bring membranes into contact through pearl chain formation, had a detectable promoting effect on fusion zone expansion. Two new dielectrophoresis protocols were used in the present work to utilize this externally generated and controllable microforce field to probe the forces intrinsic to the system that drives the expansion of the fusion zone. First, fusion zones expanded to a greater diameter in a strong AC field compared to a weak AC field, and they expanded to a greater diameter if erythrocyte ghosts received a prior heat treatment (42 degrees C, 20 min). Furthermore, flat diaphragm fusion zones broke down into open lumen fusion zones sooner (i.e., had shorter lifetimes) when they were expanding more quickly. Second, changing the AC field strength at specific times during the fusion zone expansion led to an immediate visco-elastic response. However, shifting the AC field strength to zero after 5 s of fusion zone expansion resulted in a subsequent decrease in the average fusion zone diameter. This suggests not only that the spectrin-based membrane skeleton actually tends to prevent the rounding up process but that it may be capable of generating an antirounding force, which has broad implications for the role of the membrane skeleton in cell fusion. These results are consistent with the hypothesis that flat diaphragm fusion zones induced in heat-treated membranes were very easily stretched and that membrane-based forces that control or drive the expansion process must originate from membrane area that is outside rather than inside the fusion zone. Lastly, when an outward-directed osmotic pressure-based microforce was present at the time that erythrocyte ghosts were fused, the fusion zone diameter underwent a greater expansion in the 0-1 s interval after fusion. This suggests that an osmotic pressure-based microforce can be used to experimentally calibrate the dielectrophoretic force." @default.
• W2088906085 created "2016-06-24" @default.
• W2088906085 creator A5047296388 @default.
• W2088906085 date "1995-12-01" @default.
• W2088906085 modified "2023-09-26" @default.
• W2088906085 title "Membrane skeleton restraint of surface shape change during fusion of erythrocyte membranes: evidence from use of osmotic and dielectrophoretic microforces as probes" @default.
• W2088906085 cites W1496313598 @default.
• W2088906085 cites W1544458404 @default.
• W2088906085 cites W1867649178 @default.
• W2088906085 cites W1966201108 @default.
• W2088906085 cites W1968563729 @default.
• W2088906085 cites W1972647868 @default.
• W2088906085 cites W1998958973 @default.
• W2088906085 cites W2011089056 @default.
• W2088906085 cites W2014402961 @default.
• W2088906085 cites W2024586659 @default.
• W2088906085 cites W2025150927 @default.
• W2088906085 cites W2033443055 @default.
• W2088906085 cites W2033954774 @default.
• W2088906085 cites W2036574858 @default.
• W2088906085 cites W2041929512 @default.
• W2088906085 cites W2045212194 @default.
• W2088906085 cites W2046535546 @default.
• W2088906085 cites W2050216221 @default.
• W2088906085 cites W2051345355 @default.
• W2088906085 cites W2053758185 @default.
• W2088906085 cites W2061298927 @default.
• W2088906085 cites W2066900358 @default.
• W2088906085 cites W2070129920 @default.
• W2088906085 cites W2071296282 @default.
• W2088906085 cites W2072856453 @default.
• W2088906085 cites W2082781273 @default.
• W2088906085 cites W2087704505 @default.
• W2088906085 cites W2090929307 @default.
• W2088906085 cites W2091443281 @default.
• W2088906085 cites W2096591729 @default.
• W2088906085 cites W2097464186 @default.
• W2088906085 cites W2105539899 @default.
• W2088906085 cites W2111828533 @default.
• W2088906085 cites W2125954910 @default.
• W2088906085 cites W2135770620 @default.
• W2088906085 cites W2146972602 @default.
• W2088906085 cites W2159689005 @default.
• W2088906085 cites W2160906826 @default.
• W2088906085 cites W2298330185 @default.
• W2088906085 cites W2419365886 @default.
• W2088906085 cites W2432136139 @default.
• W2088906085 cites W2437263680 @default.
• W2088906085 cites W2441045304 @default.
• W2088906085 cites W4211239492 @default.
• W2088906085 cites W4234272655 @default.
• W2088906085 cites W2116549174 @default.
• W2088906085 doi "https://doi.org/10.1016/s0006-3495(95)80121-9" @default.
• W2088906085 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1236488" @default.
• W2088906085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8599657" @default.
• W2088906085 hasPublicationYear "1995" @default.
• W2088906085 type Work @default.
• W2088906085 sameAs 2088906085 @default.
• W2088906085 citedByCount "10" @default.
• W2088906085 countsByYear W20889060852014 @default.
• W2088906085 crossrefType "journal-article" @default.
• W2088906085 hasAuthorship W2088906085A5047296388 @default.
• W2088906085 hasBestOaLocation W20889060851 @default.
• W2088906085 hasConcept C103038307 @default.
• W2088906085 hasConcept C106825181 @default.
• W2088906085 hasConcept C12554922 @default.
• W2088906085 hasConcept C138885662 @default.
• W2088906085 hasConcept C142669718 @default.
• W2088906085 hasConcept C1491633281 @default.
• W2088906085 hasConcept C158525013 @default.
• W2088906085 hasConcept C171250308 @default.
• W2088906085 hasConcept C185592680 @default.
• W2088906085 hasConcept C192562407 @default.
• W2088906085 hasConcept C41625074 @default.
• W2088906085 hasConcept C41895202 @default.
• W2088906085 hasConcept C50244902 @default.
• W2088906085 hasConcept C55493867 @default.
• W2088906085 hasConcept C8673954 @default.
• W2088906085 hasConcept C86803240 @default.
• W2088906085 hasConcept C92488253 @default.
• W2088906085 hasConceptScore W2088906085C103038307 @default.
• W2088906085 hasConceptScore W2088906085C106825181 @default.
• W2088906085 hasConceptScore W2088906085C12554922 @default.
• W2088906085 hasConceptScore W2088906085C138885662 @default.
• W2088906085 hasConceptScore W2088906085C142669718 @default.
• W2088906085 hasConceptScore W2088906085C1491633281 @default.
• W2088906085 hasConceptScore W2088906085C158525013 @default.
• W2088906085 hasConceptScore W2088906085C171250308 @default.
• W2088906085 hasConceptScore W2088906085C185592680 @default.
• W2088906085 hasConceptScore W2088906085C192562407 @default.
• W2088906085 hasConceptScore W2088906085C41625074 @default.
• W2088906085 hasConceptScore W2088906085C41895202 @default.
• W2088906085 hasConceptScore W2088906085C50244902 @default.
• W2088906085 hasConceptScore W2088906085C55493867 @default.
• W2088906085 hasConceptScore W2088906085C8673954 @default.
• W2088906085 hasConceptScore W2088906085C86803240 @default.
• W2088906085 hasConceptScore W2088906085C92488253 @default.
• W2088906085 hasLocation W20889060851 @default.
• W2088906085 hasLocation W20889060852 @default.
• W2088906085 hasLocation W20889060853 @default.

• next