FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088976989> ?p ?o ?g. }

• W2088976989 endingPage "59" @default.
• W2088976989 startingPage "51" @default.
• W2088976989 abstract "Human basophils are an accessible participant of the human allergic reaction. There is natural variation in various functional endpoints and in signaling molecule expression but there has been only a limited effort to place this information in the context of mRNA expression profiles. The goal of these studies was to develop gene transcript profiles associated with IL-3 exposure for use in probing basophil phenotypes. Highly purified human basophils were cultured under several conditions and the cells subsequently analyzed by microarray analysis of gene transcript expression. Basophils were cultured with or without IL-3 for 24 h and the expression profile, relative to pre-culture, analyzed for transcripts that act in a reciprocal fashion depending on the condition. A 51-gene transcript set was identified that was composed of 34 transcripts that increased with IL-3 and decreased without IL-3 and a further 17 transcripts that did the reverse. This set of transcripts was validated on two microarray chips. The changes induced by IL-3 were stable in time, with 3 day cultures generating a signature concordant with 1 day cultures. The inverted nature of the response to ± IL-3 suggested that the normal circulating basophil is balanced between a state of high and low IL-3 exposure and thus is very sensitive to changes in IL-3. For example, the basophil could detect the early generation of IL-3 that follows IgE-mediated stimulation of basophils. When this signature tool was applied to freshly isolated basophils, it was observed that the signature was similar for the same subjects’ basophil sampled weeks apart. It was also shown that the 51-gene transcript was insensitive to the method of preparing purified basophils. Finally, these studies provided an estimate for the normal in vivo exposure of circulating basophils to IL-3 or IL-3-like functionality. These studies identified an IL-3 signature to probe changes in basophils occurring in vivo." @default.
• W2088976989 created "2016-06-24" @default.
• W2088976989 creator A5081046119 @default.
• W2088976989 date "2012-11-01" @default.
• W2088976989 modified "2023-10-18" @default.
• W2088976989 title "Development of a microarray-based method to detect exposure of human basophils to IL-3" @default.
• W2088976989 cites W1525177329 @default.
• W2088976989 cites W1574590245 @default.
• W2088976989 cites W1595101033 @default.
• W2088976989 cites W1676258780 @default.
• W2088976989 cites W1972312838 @default.
• W2088976989 cites W1972766662 @default.
• W2088976989 cites W1986233034 @default.
• W2088976989 cites W1993207743 @default.
• W2088976989 cites W2010748354 @default.
• W2088976989 cites W2017810034 @default.
• W2088976989 cites W2022461208 @default.
• W2088976989 cites W2027919962 @default.
• W2088976989 cites W2031767694 @default.
• W2088976989 cites W2034248197 @default.
• W2088976989 cites W2035700261 @default.
• W2088976989 cites W2046914591 @default.
• W2088976989 cites W2053770531 @default.
• W2088976989 cites W2055902826 @default.
• W2088976989 cites W2067504363 @default.
• W2088976989 cites W2093442991 @default.
• W2088976989 cites W2118590252 @default.
• W2088976989 cites W2128553167 @default.
• W2088976989 cites W2135992160 @default.
• W2088976989 cites W2160603692 @default.
• W2088976989 doi "https://doi.org/10.1016/j.jim.2012.08.006" @default.
• W2088976989 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3505599" @default.
• W2088976989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22921732" @default.
• W2088976989 hasPublicationYear "2012" @default.
• W2088976989 type Work @default.
• W2088976989 sameAs 2088976989 @default.
• W2088976989 citedByCount "7" @default.
• W2088976989 countsByYear W20889769892012 @default.
• W2088976989 countsByYear W20889769892013 @default.
• W2088976989 countsByYear W20889769892014 @default.
• W2088976989 countsByYear W20889769892015 @default.
• W2088976989 countsByYear W20889769892020 @default.
• W2088976989 countsByYear W20889769892021 @default.
• W2088976989 countsByYear W20889769892023 @default.
• W2088976989 crossrefType "journal-article" @default.
• W2088976989 hasAuthorship W2088976989A5081046119 @default.
• W2088976989 hasBestOaLocation W20889769892 @default.
• W2088976989 hasConcept C104317684 @default.
• W2088976989 hasConcept C105580179 @default.
• W2088976989 hasConcept C141105273 @default.
• W2088976989 hasConcept C150194340 @default.
• W2088976989 hasConcept C151730666 @default.
• W2088976989 hasConcept C153911025 @default.
• W2088976989 hasConcept C159654299 @default.
• W2088976989 hasConcept C18431079 @default.
• W2088976989 hasConcept C186836561 @default.
• W2088976989 hasConcept C203014093 @default.
• W2088976989 hasConcept C2777456892 @default.
• W2088976989 hasConcept C2779343474 @default.
• W2088976989 hasConcept C54355233 @default.
• W2088976989 hasConcept C8415881 @default.
• W2088976989 hasConcept C86803240 @default.
• W2088976989 hasConcept C95444343 @default.
• W2088976989 hasConceptScore W2088976989C104317684 @default.
• W2088976989 hasConceptScore W2088976989C105580179 @default.
• W2088976989 hasConceptScore W2088976989C141105273 @default.
• W2088976989 hasConceptScore W2088976989C150194340 @default.
• W2088976989 hasConceptScore W2088976989C151730666 @default.
• W2088976989 hasConceptScore W2088976989C153911025 @default.
• W2088976989 hasConceptScore W2088976989C159654299 @default.
• W2088976989 hasConceptScore W2088976989C18431079 @default.
• W2088976989 hasConceptScore W2088976989C186836561 @default.
• W2088976989 hasConceptScore W2088976989C203014093 @default.
• W2088976989 hasConceptScore W2088976989C2777456892 @default.
• W2088976989 hasConceptScore W2088976989C2779343474 @default.
• W2088976989 hasConceptScore W2088976989C54355233 @default.
• W2088976989 hasConceptScore W2088976989C8415881 @default.
• W2088976989 hasConceptScore W2088976989C86803240 @default.
• W2088976989 hasConceptScore W2088976989C95444343 @default.
• W2088976989 hasIssue "1-2" @default.
• W2088976989 hasLocation W20889769891 @default.
• W2088976989 hasLocation W20889769892 @default.
• W2088976989 hasLocation W20889769893 @default.
• W2088976989 hasLocation W20889769894 @default.
• W2088976989 hasOpenAccess W2088976989 @default.
• W2088976989 hasPrimaryLocation W20889769891 @default.
• W2088976989 hasRelatedWork W1531528770 @default.
• W2088976989 hasRelatedWork W1971076179 @default.
• W2088976989 hasRelatedWork W2053053668 @default.
• W2088976989 hasRelatedWork W2112336316 @default.
• W2088976989 hasRelatedWork W2392221252 @default.
• W2088976989 hasRelatedWork W2439513411 @default.
• W2088976989 hasRelatedWork W262379139 @default.
• W2088976989 hasRelatedWork W2773571775 @default.
• W2088976989 hasRelatedWork W2782128209 @default.
• W2088976989 hasRelatedWork W3032874291 @default.
• W2088976989 hasVolume "385" @default.
• W2088976989 isParatext "false" @default.

• next