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- W2088988522 abstract "Abstract The presence of sulfated bile acid in urine of patients with hepatobiliary diseases was recognized by using an Amberlite XAD‐2 column for extraction of bile acid and a Sephadex LH‐20 column for separation of sulfated (sulfate of either taurine or glycine conjugate) and nonsulfated bile acid (taurine and glycine conjugate). Sulfated and nonsulfated bile acid, obtained after a Sephadex LH‐20 column of urinary extract of the patient with acute hepatitis, was identified by thin layer chromatography. Sulfated bile acid showed a spot with different Rf value from that of taurine‐conjugated, glycine‐conjugated and free bile acid, and solvolysis of sulfated bile acid resulted in a compound with the same Rf value as glycodihydroxycholanoic acid. A large amount of bile acid sulfate was found in urine of patients with hepatobiliary diseases. The sulfated bile acid in these urine samples occupied from 57.1 to 93.3% of total bile acid, and consisted of both di‐and trihydroxycholanoic acid (major part, chenodeoxycholic acid). As no solvolysis was carried out in previous works, bile acid sulfate in urine, as described in this paper, was not determined at all." @default.
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- W2088988522 date "1973-01-01" @default.
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- W2088988522 title "Sulfated bile acid in urine of patients with hepatobiliary diseases" @default.
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- W2088988522 doi "https://doi.org/10.1007/bf02533240" @default.
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