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- W2088990532 endingPage "e1001186" @default.
- W2088990532 startingPage "e1001186" @default.
- W2088990532 abstract "Paramyxoviruses are known to replicate in the cytoplasm and bud from the plasma membrane. Matrix is the major structural protein in paramyxoviruses that mediates viral assembly and budding. Curiously, the matrix proteins of a few paramyxoviruses have been found in the nucleus, although the biological function associated with this nuclear localization remains obscure. We report here that the nuclear-cytoplasmic trafficking of the Nipah virus matrix (NiV-M) protein and associated post-translational modification play a critical role in matrix-mediated virus budding. Nipah virus (NiV) is a highly pathogenic emerging paramyxovirus that causes fatal encephalitis in humans, and is classified as a Biosafety Level 4 (BSL4) pathogen. During live NiV infection, NiV-M was first detected in the nucleus at early stages of infection before subsequent localization to the cytoplasm and the plasma membrane. Mutations in the putative bipartite nuclear localization signal (NLS) and the leucine-rich nuclear export signal (NES) found in NiV-M impaired its nuclear-cytoplasmic trafficking and also abolished NiV-M budding. A highly conserved lysine residue in the NLS served dual functions: its positive charge was important for mediating nuclear import, and it was also a potential site for monoubiquitination which regulates nuclear export of the protein. Concordantly, overexpression of ubiquitin enhanced NiV-M budding whereas depletion of free ubiquitin in the cell (via proteasome inhibitors) resulted in nuclear retention of NiV-M and blocked viral budding. Live Nipah virus budding was exquisitely sensitive to proteasome inhibitors: bortezomib, an FDA-approved proteasome inhibitor for treating multiple myeloma, reduced viral titers with an IC50 of 2.7 nM, which is 100-fold less than the peak plasma concentration that can be achieved in humans. This opens up the possibility of using an “off-the-shelf” therapeutic against acute NiV infection." @default.
- W2088990532 created "2016-06-24" @default.
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- W2088990532 date "2010-11-11" @default.
- W2088990532 modified "2023-10-10" @default.
- W2088990532 title "Ubiquitin-Regulated Nuclear-Cytoplasmic Trafficking of the Nipah Virus Matrix Protein Is Important for Viral Budding" @default.
- W2088990532 cites W1497941038 @default.
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- W2088990532 cites W1562998360 @default.
- W2088990532 cites W1791142192 @default.
- W2088990532 cites W1890859862 @default.
- W2088990532 cites W1962465787 @default.
- W2088990532 cites W1965578845 @default.
- W2088990532 cites W1972444714 @default.
- W2088990532 cites W1974802738 @default.
- W2088990532 cites W1978614903 @default.
- W2088990532 cites W1984587504 @default.
- W2088990532 cites W1985104611 @default.
- W2088990532 cites W1985641201 @default.
- W2088990532 cites W1988824816 @default.
- W2088990532 cites W1991153512 @default.
- W2088990532 cites W1992482773 @default.
- W2088990532 cites W1994806672 @default.
- W2088990532 cites W2003903997 @default.
- W2088990532 cites W2008787934 @default.
- W2088990532 cites W2009552582 @default.
- W2088990532 cites W2009754558 @default.
- W2088990532 cites W2014812468 @default.
- W2088990532 cites W2015256176 @default.
- W2088990532 cites W2015703878 @default.
- W2088990532 cites W2016949937 @default.
- W2088990532 cites W2019525085 @default.
- W2088990532 cites W2021176126 @default.
- W2088990532 cites W2028885314 @default.
- W2088990532 cites W2030275229 @default.
- W2088990532 cites W2039055702 @default.
- W2088990532 cites W2042499956 @default.
- W2088990532 cites W2044568075 @default.
- W2088990532 cites W2050745715 @default.
- W2088990532 cites W2053497997 @default.
- W2088990532 cites W2058035064 @default.
- W2088990532 cites W2060316418 @default.
- W2088990532 cites W2070363128 @default.
- W2088990532 cites W2074177924 @default.
- W2088990532 cites W2077482382 @default.
- W2088990532 cites W2080813177 @default.
- W2088990532 cites W2081747066 @default.
- W2088990532 cites W2086725260 @default.
- W2088990532 cites W2095659495 @default.
- W2088990532 cites W2105249930 @default.
- W2088990532 cites W2110942624 @default.
- W2088990532 cites W2112190271 @default.
- W2088990532 cites W2122684890 @default.
- W2088990532 cites W2127949919 @default.
- W2088990532 cites W2128647460 @default.
- W2088990532 cites W2128780965 @default.
- W2088990532 cites W2133682271 @default.
- W2088990532 cites W2136284743 @default.
- W2088990532 cites W2143111754 @default.
- W2088990532 cites W2145075118 @default.
- W2088990532 cites W2146491521 @default.
- W2088990532 cites W2147093282 @default.
- W2088990532 cites W2152215476 @default.
- W2088990532 cites W2167648539 @default.
- W2088990532 cites W2167971272 @default.
- W2088990532 cites W2169146746 @default.
- W2088990532 cites W2169388729 @default.
- W2088990532 cites W2170128965 @default.
- W2088990532 cites W2170851459 @default.
- W2088990532 cites W4249167162 @default.
- W2088990532 cites W4249774679 @default.
- W2088990532 cites W4267221 @default.
- W2088990532 doi "https://doi.org/10.1371/journal.ppat.1001186" @default.
- W2088990532 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2978725" @default.
- W2088990532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21085610" @default.
- W2088990532 hasPublicationYear "2010" @default.
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