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- W2088993146 abstract "Synchronisation of the Trypanosoma brucei cell cycle proved elusive for many years. A recent report demonstrated that synchronisation of procyclic form cells was possible following treatment with hydroxyurea. Here, that work is extended to the disease-relevant, mammalian-infective bloodstream stage trypanosome. Treatment of bloodstream stage Lister 427 T. brucei cells growing in vitro with 10 μg ml−1 hydroxyurea for 6 h led to an enrichment of cells in S phase. Following removal of the drug, cells proceeded uniformly through one round of the cell cycle, providing a much needed tool to enrich for specific cell cycle stages, in a manner similar to hydroxyurea treatment of procyclic form T. brucei." @default.
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- W2088993146 date "2009-04-01" @default.
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- W2088993146 title "Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei" @default.
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- W2088993146 doi "https://doi.org/10.1016/j.molbiopara.2008.12.008" @default.
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