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- W2088994405 abstract "1. Adult females of the stick insect Carausius morosus were injected with either [35S]methionine or [3H]leucine and eggs laid during the following 2 weeks collected for further analysis. 2. After the injection, radioactivity started to appear in newly laid eggs with a time-lag of 9 days, reached a maximum incorporation on day 10 and then levelled off, due to dilution of the injected tracer. 3. Newly laid eggs from injected females were analyzed by gel electrophoresis and the incorporated radioactivity revealed fluorographically. 4. Data showed that vitellin polypeptides were the only egg constituent to become labelled under these conditions. 5. In addition, light microscope autoradiography proved that the radioactivity incorporated in vivo into ovarian follicles and eggs was restricted to the forming yolk spheres or to the central ooplasm, respectively. 6. Radioactive embryos were allowed to develop and the polypeptides appearing during development identified fluorographically on SDS gels. 7. To establish whether these newly appearing polypeptides were due to de novo synthesis or to fragments of pre-existing vitellin polypeptides, embryos were also cultured in vitro in the presence of the above radioactive amino acids. 8. These conditions allowed newly synthesized proteins to be identified in both native and SDS gels and to be distinguished from the vitellin polypeptides labelled in vivo." @default.
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- W2088994405 date "1990-01-01" @default.
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- W2088994405 title "Changes in the patterns of proteins stored and synthesized by developing embryos of the stick insect Carausius morosus Br" @default.
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- W2088994405 doi "https://doi.org/10.1016/0305-0491(90)90255-r" @default.
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