FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089035537> ?p ?o ?g. }

• W2089035537 endingPage "e67699" @default.
• W2089035537 startingPage "e67699" @default.
• W2089035537 abstract "Culture-independent high-throughput sequencing-based methods are widely used to study bacterial communities. Although these approaches are superior to traditional culture-based methods, they introduce bias at the experimental and bioinformatics levels. We assessed the diversity of the human salivary microbiome by pyrosequencing of the 16S rDNA V1–3 amplicons using metagenomic DNA extracted by two different protocols: a simple proteinase K digestion without a subsequent DNA clean-up step, and a bead-beating mechanical lysis protocol followed by column DNA purification. A high degree of congruence was found between the two extraction methods, most notably in regard to the microbial community composition. The results showed that for a given bioinformatics pipeline, all the taxa with an average proportion >0.12% in samples processed using one extraction method were also detected in samples extracted using the other method. The same taxa tended to be abundant and frequent for both extraction methods. The relative abundance of sequence reads assigned to the phyla Actinobacteria, Spirochaetes, TM7, Synergistetes, and Tenericutes was significantly higher in the mechanically-treated samples than in the enzymatically-treated samples, whereas the phylum Firmicutes showed the opposite pattern. No significant differences in diversity indices were found between the extraction methods, although the mechanical lysis method revealed higher operational taxonomic unit richness. Differences between the extraction procedures outweighed the variations due to the bioinformatics analysis pipelines used." @default.
• W2089035537 created "2016-06-24" @default.
• W2089035537 creator A5004068707 @default.
• W2089035537 creator A5010055797 @default.
• W2089035537 creator A5045080230 @default.
• W2089035537 creator A5059773554 @default.
• W2089035537 creator A5088214706 @default.
• W2089035537 date "2013-07-03" @default.
• W2089035537 modified "2023-10-17" @default.
• W2089035537 title "Comparison of DNA Extraction Methods in Analysis of Salivary Bacterial Communities" @default.
• W2089035537 cites W1970063271 @default.
• W2089035537 cites W1978644187 @default.
• W2089035537 cites W1990502651 @default.
• W2089035537 cites W1994390868 @default.
• W2089035537 cites W1998469049 @default.
• W2089035537 cites W2004290014 @default.
• W2089035537 cites W2031611770 @default.
• W2089035537 cites W2038838030 @default.
• W2089035537 cites W2055043387 @default.
• W2089035537 cites W2055615060 @default.
• W2089035537 cites W2062485974 @default.
• W2089035537 cites W2077566184 @default.
• W2089035537 cites W2083153149 @default.
• W2089035537 cites W2090110425 @default.
• W2089035537 cites W2095135825 @default.
• W2089035537 cites W2095175502 @default.
• W2089035537 cites W2095566926 @default.
• W2089035537 cites W2097606916 @default.
• W2089035537 cites W2101120467 @default.
• W2089035537 cites W2103805072 @default.
• W2089035537 cites W2107677337 @default.
• W2089035537 cites W2108718991 @default.
• W2089035537 cites W2110065044 @default.
• W2089035537 cites W2115699469 @default.
• W2089035537 cites W2117522399 @default.
• W2089035537 cites W2136879569 @default.
• W2089035537 cites W2141254741 @default.
• W2089035537 cites W2144069576 @default.
• W2089035537 cites W2147618390 @default.
• W2089035537 cites W2149426290 @default.
• W2089035537 cites W2149824014 @default.
• W2089035537 cites W2150840120 @default.
• W2089035537 cites W2150914285 @default.
• W2089035537 cites W2152885278 @default.
• W2089035537 cites W2154026962 @default.
• W2089035537 cites W2155066423 @default.
• W2089035537 cites W2156536854 @default.
• W2089035537 doi "https://doi.org/10.1371/journal.pone.0067699" @default.
• W2089035537 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3701005" @default.
• W2089035537 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23844068" @default.
• W2089035537 hasPublicationYear "2013" @default.
• W2089035537 type Work @default.
• W2089035537 sameAs 2089035537 @default.
• W2089035537 citedByCount "72" @default.
• W2089035537 countsByYear W20890355372013 @default.
• W2089035537 countsByYear W20890355372014 @default.
• W2089035537 countsByYear W20890355372015 @default.
• W2089035537 countsByYear W20890355372016 @default.
• W2089035537 countsByYear W20890355372017 @default.
• W2089035537 countsByYear W20890355372018 @default.
• W2089035537 countsByYear W20890355372019 @default.
• W2089035537 countsByYear W20890355372020 @default.
• W2089035537 countsByYear W20890355372021 @default.
• W2089035537 countsByYear W20890355372022 @default.
• W2089035537 countsByYear W20890355372023 @default.
• W2089035537 crossrefType "journal-article" @default.
• W2089035537 hasAuthorship W2089035537A5004068707 @default.
• W2089035537 hasAuthorship W2089035537A5010055797 @default.
• W2089035537 hasAuthorship W2089035537A5045080230 @default.
• W2089035537 hasAuthorship W2089035537A5059773554 @default.
• W2089035537 hasAuthorship W2089035537A5088214706 @default.
• W2089035537 hasBestOaLocation W20890355371 @default.
• W2089035537 hasConcept C104317684 @default.
• W2089035537 hasConcept C125009961 @default.
• W2089035537 hasConcept C143121216 @default.
• W2089035537 hasConcept C15151743 @default.
• W2089035537 hasConcept C153911025 @default.
• W2089035537 hasConcept C185592680 @default.
• W2089035537 hasConcept C2777695942 @default.
• W2089035537 hasConcept C2778169975 @default.
• W2089035537 hasConcept C2779825944 @default.
• W2089035537 hasConcept C42062724 @default.
• W2089035537 hasConcept C43617362 @default.
• W2089035537 hasConcept C4725764 @default.
• W2089035537 hasConcept C49105822 @default.
• W2089035537 hasConcept C523546767 @default.
• W2089035537 hasConcept C54355233 @default.
• W2089035537 hasConcept C57409179 @default.
• W2089035537 hasConcept C78063203 @default.
• W2089035537 hasConcept C8185291 @default.
• W2089035537 hasConcept C86803240 @default.
• W2089035537 hasConcept C91066513 @default.
• W2089035537 hasConceptScore W2089035537C104317684 @default.
• W2089035537 hasConceptScore W2089035537C125009961 @default.
• W2089035537 hasConceptScore W2089035537C143121216 @default.
• W2089035537 hasConceptScore W2089035537C15151743 @default.
• W2089035537 hasConceptScore W2089035537C153911025 @default.
• W2089035537 hasConceptScore W2089035537C185592680 @default.

• next