FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089040893> ?p ?o ?g. }

• W2089040893 endingPage "544" @default.
• W2089040893 startingPage "535" @default.
• W2089040893 abstract "Mutants isolated from the Y1 mouse adrenocortical tumor cell line (clones 10r-9 and 10r-6) are resistant to ACTH because they fail to express the melanocortin-2 receptor (MC2R). In this study, we show that a luciferase reporter plasmid driven by 1800 bp of the proximal promoter region of the MC2R was expressed poorly in the mutant cells compared with parent Y1 cells. The differential expression of the MC2R in parent and mutant cells resulted from impaired activity of the orphan nuclear receptor NR5A1 (SF1) on the promoter as determined by 5′-deletion analysis. Furthermore, the activity of an SF1 expression plasmid on an SF1-dependent reporter plasmid was compromised in mutant clones. The site-specific DNA binding properties of SF1 from parent and mutant cells did not differ as determined in electrophoretic mobility shift assays, and the addition of the activation domain of VP16 to the amino terminus of SF1 restored the transcriptional activity of the protein. In addition, the levels of SF1 and other cofactors including WT1, CBP/p300, and steroid receptor coactivator 1 did not differ appreciably between parent and mutant cells. Taken together, these results suggest that ACTH resistance in the mutant clones resulted from a defect that affected the activation properties of SF1 rather than its DNA binding activity. Consistent with the observed impairment in SF1 function, other SF1-dependent genes, including Cyp11b1 and steroidogenic acute regulatory protein (StAR), were poorly expressed and global steroidogenesis, as evidenced by the metabolism of 22(R)-hydroxycholesterol to steroid products, was impaired. Interestingly, MC2R, Cyp11a, Cyp11b1, and StAR transcripts were not affected to the same degree, suggesting that each of these genes may have a different absolute requirement for SF1. These mutants thus provide an experimental paradigm to identify factors that influence SF1 function and to evaluate the relative importance of SF1 in the expression of genes essential for adrenal steroidogenesis." @default.
• W2089040893 created "2016-06-24" @default.
• W2089040893 creator A5058765237 @default.
• W2089040893 creator A5071796896 @default.
• W2089040893 creator A5078780767 @default.
• W2089040893 creator A5083379860 @default.
• W2089040893 date "2000-04-01" @default.
• W2089040893 modified "2023-09-27" @default.
• W2089040893 title "Impaired Steroidogenic Factor 1 (NR5A1) Activity in Mutant Y1 Mouse Adrenocortical Tumor Cells" @default.
• W2089040893 cites W1031388349 @default.
• W2089040893 cites W1482432683 @default.
• W2089040893 cites W1556752176 @default.
• W2089040893 cites W1568734886 @default.
• W2089040893 cites W1597372220 @default.
• W2089040893 cites W1847582680 @default.
• W2089040893 cites W1947355021 @default.
• W2089040893 cites W1965372133 @default.
• W2089040893 cites W1984212762 @default.
• W2089040893 cites W2003461797 @default.
• W2089040893 cites W2015149771 @default.
• W2089040893 cites W2040687221 @default.
• W2089040893 cites W2047350170 @default.
• W2089040893 cites W2050695301 @default.
• W2089040893 cites W2054736204 @default.
• W2089040893 cites W2062847609 @default.
• W2089040893 cites W2067981937 @default.
• W2089040893 cites W2072386144 @default.
• W2089040893 cites W2074100845 @default.
• W2089040893 cites W2078503449 @default.
• W2089040893 cites W2079059309 @default.
• W2089040893 cites W2082685929 @default.
• W2089040893 cites W2095362827 @default.
• W2089040893 cites W2097794242 @default.
• W2089040893 cites W2097890006 @default.
• W2089040893 cites W2100837269 @default.
• W2089040893 cites W2100859941 @default.
• W2089040893 cites W2111924561 @default.
• W2089040893 cites W2156744272 @default.
• W2089040893 cites W2171226274 @default.
• W2089040893 cites W98498425 @default.
• W2089040893 doi "https://doi.org/10.1210/mend.14.4.0440" @default.
• W2089040893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10770490" @default.
• W2089040893 hasPublicationYear "2000" @default.
• W2089040893 type Work @default.
• W2089040893 sameAs 2089040893 @default.
• W2089040893 citedByCount "23" @default.
• W2089040893 countsByYear W20890408932014 @default.
• W2089040893 countsByYear W20890408932015 @default.
• W2089040893 countsByYear W20890408932016 @default.
• W2089040893 countsByYear W20890408932017 @default.
• W2089040893 countsByYear W20890408932023 @default.
• W2089040893 crossrefType "journal-article" @default.
• W2089040893 hasAuthorship W2089040893A5058765237 @default.
• W2089040893 hasAuthorship W2089040893A5071796896 @default.
• W2089040893 hasAuthorship W2089040893A5078780767 @default.
• W2089040893 hasAuthorship W2089040893A5083379860 @default.
• W2089040893 hasBestOaLocation W20890408931 @default.
• W2089040893 hasConcept C104317684 @default.
• W2089040893 hasConcept C143065580 @default.
• W2089040893 hasConcept C150194340 @default.
• W2089040893 hasConcept C153911025 @default.
• W2089040893 hasConcept C161733203 @default.
• W2089040893 hasConcept C183978625 @default.
• W2089040893 hasConcept C2777703421 @default.
• W2089040893 hasConcept C33987129 @default.
• W2089040893 hasConcept C54355233 @default.
• W2089040893 hasConcept C63932345 @default.
• W2089040893 hasConcept C77445288 @default.
• W2089040893 hasConcept C86339819 @default.
• W2089040893 hasConcept C86803240 @default.
• W2089040893 hasConceptScore W2089040893C104317684 @default.
• W2089040893 hasConceptScore W2089040893C143065580 @default.
• W2089040893 hasConceptScore W2089040893C150194340 @default.
• W2089040893 hasConceptScore W2089040893C153911025 @default.
• W2089040893 hasConceptScore W2089040893C161733203 @default.
• W2089040893 hasConceptScore W2089040893C183978625 @default.
• W2089040893 hasConceptScore W2089040893C2777703421 @default.
• W2089040893 hasConceptScore W2089040893C33987129 @default.
• W2089040893 hasConceptScore W2089040893C54355233 @default.
• W2089040893 hasConceptScore W2089040893C63932345 @default.
• W2089040893 hasConceptScore W2089040893C77445288 @default.
• W2089040893 hasConceptScore W2089040893C86339819 @default.
• W2089040893 hasConceptScore W2089040893C86803240 @default.
• W2089040893 hasIssue "4" @default.
• W2089040893 hasLocation W20890408931 @default.
• W2089040893 hasLocation W20890408932 @default.
• W2089040893 hasOpenAccess W2089040893 @default.
• W2089040893 hasPrimaryLocation W20890408931 @default.
• W2089040893 hasRelatedWork W1974663161 @default.
• W2089040893 hasRelatedWork W2000441118 @default.
• W2089040893 hasRelatedWork W2035043182 @default.
• W2089040893 hasRelatedWork W2089040893 @default.
• W2089040893 hasRelatedWork W2096550567 @default.
• W2089040893 hasRelatedWork W2118867779 @default.
• W2089040893 hasRelatedWork W2119377878 @default.
• W2089040893 hasRelatedWork W2125309269 @default.
• W2089040893 hasRelatedWork W4234372104 @default.
• W2089040893 hasRelatedWork W4321097001 @default.

• next