FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089113485> ?p ?o ?g. }

• W2089113485 endingPage "578" @default.
• W2089113485 startingPage "571" @default.
• W2089113485 abstract "An endoplasmic-reticulum — DNA-polymerase complex was prepared from unfertilized sea urchin eggs and its DNA-synthesizing activity was examined using single-stranded DNA of bacteriophage fd as a template. The complex catalyzed the ribonucleotide-dependent DNA synthesis which required dNTPs, NTPs, Mg2+ and single-stranded DNA. The DNA synthesis was sensitive to aphidicolin and N-ethylmaleimide but was resistant to 2′,3′-dideoxyribosylthymine 5′-triphosphate (ddTTP) and α-amanitin, suggesting the involvement of DNA polymerase α. In parallel with the DNA synthesis, a small amount of RNA was synthesized in the presence of 100 μg/ml α-amanitin. The Km value of ribonucleotides for the RNA synthesis coincided with that for the DNA synthesis, suggesting a correlation between the DNA and RNA syntheses. Labelling of the products with [γ-32P]ATP followed by DNA digestion with pancreatic DNase I revealed the attachment of an oligoribonucleotide (7–11 bases in length) at the 5′ ends of the DNA products. These observations suggest that in DNA synthesis, primer RNA synthesis occurs first, followed by DNA chain elongation. During 1–90-min incubation, the amount of the DNA synthesized increased but the length was not significantly increased. Over 80% of the number of synthesized DNA molecules comprised a single population of short DNA fragments (60–200 bases, on average 120 bases in length) and the number of fragments increased, depending on the incubation time. However, DNA fragments of various sizes (about 100–6000 bases) were synthesized with DNA polymerase α solubilized from the endoplasmic-reticulum — DNA-polymerase complex. All this evidence suggests that in vitro, the complex preferentially synthesizes a particular size of short DNA fragments. The significance of the fragments is discussed." @default.
• W2089113485 created "2016-06-24" @default.
• W2089113485 creator A5005341788 @default.
• W2089113485 date "1986-11-01" @default.
• W2089113485 modified "2023-10-02" @default.
• W2089113485 title "DNA synthesis in vitro with an endoplasmic-reticulum-DNA-polymerase complex from unfertilized sea urchin eggs" @default.
• W2089113485 cites W140926695 @default.
• W2089113485 cites W1485247673 @default.
• W2089113485 cites W1487826023 @default.
• W2089113485 cites W1498433985 @default.
• W2089113485 cites W1524532519 @default.
• W2089113485 cites W1544754530 @default.
• W2089113485 cites W1560333621 @default.
• W2089113485 cites W1565663925 @default.
• W2089113485 cites W1579328338 @default.
• W2089113485 cites W1587694577 @default.
• W2089113485 cites W1964547239 @default.
• W2089113485 cites W1965397839 @default.
• W2089113485 cites W1966711072 @default.
• W2089113485 cites W1967726584 @default.
• W2089113485 cites W1972533225 @default.
• W2089113485 cites W1986058259 @default.
• W2089113485 cites W1992595091 @default.
• W2089113485 cites W1996305622 @default.
• W2089113485 cites W1996497833 @default.
• W2089113485 cites W1999082616 @default.
• W2089113485 cites W2006585942 @default.
• W2089113485 cites W2008312779 @default.
• W2089113485 cites W2009323300 @default.
• W2089113485 cites W2015925955 @default.
• W2089113485 cites W2023865120 @default.
• W2089113485 cites W2029169703 @default.
• W2089113485 cites W2037476050 @default.
• W2089113485 cites W2041768220 @default.
• W2089113485 cites W2047552636 @default.
• W2089113485 cites W2059547506 @default.
• W2089113485 cites W2059878709 @default.
• W2089113485 cites W2074303557 @default.
• W2089113485 cites W2094561366 @default.
• W2089113485 cites W2112602097 @default.
• W2089113485 cites W2130386700 @default.
• W2089113485 cites W2136334201 @default.
• W2089113485 cites W2165321825 @default.
• W2089113485 cites W2179903983 @default.
• W2089113485 doi "https://doi.org/10.1111/j.1432-1033.1986.tb10076.x" @default.
• W2089113485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3780722" @default.
• W2089113485 hasPublicationYear "1986" @default.
• W2089113485 type Work @default.
• W2089113485 sameAs 2089113485 @default.
• W2089113485 citedByCount "6" @default.
• W2089113485 crossrefType "journal-article" @default.
• W2089113485 hasAuthorship W2089113485A5005341788 @default.
• W2089113485 hasConcept C104317684 @default.
• W2089113485 hasConcept C153911025 @default.
• W2089113485 hasConcept C156719811 @default.
• W2089113485 hasConcept C164965036 @default.
• W2089113485 hasConcept C178790620 @default.
• W2089113485 hasConcept C185592680 @default.
• W2089113485 hasConcept C194830204 @default.
• W2089113485 hasConcept C2756471 @default.
• W2089113485 hasConcept C2777563447 @default.
• W2089113485 hasConcept C31254050 @default.
• W2089113485 hasConcept C552990157 @default.
• W2089113485 hasConcept C55493867 @default.
• W2089113485 hasConcept C64241487 @default.
• W2089113485 hasConcept C67705224 @default.
• W2089113485 hasConcept C82381507 @default.
• W2089113485 hasConcept C86803240 @default.
• W2089113485 hasConcept C88354546 @default.
• W2089113485 hasConceptScore W2089113485C104317684 @default.
• W2089113485 hasConceptScore W2089113485C153911025 @default.
• W2089113485 hasConceptScore W2089113485C156719811 @default.
• W2089113485 hasConceptScore W2089113485C164965036 @default.
• W2089113485 hasConceptScore W2089113485C178790620 @default.
• W2089113485 hasConceptScore W2089113485C185592680 @default.
• W2089113485 hasConceptScore W2089113485C194830204 @default.
• W2089113485 hasConceptScore W2089113485C2756471 @default.
• W2089113485 hasConceptScore W2089113485C2777563447 @default.
• W2089113485 hasConceptScore W2089113485C31254050 @default.
• W2089113485 hasConceptScore W2089113485C552990157 @default.
• W2089113485 hasConceptScore W2089113485C55493867 @default.
• W2089113485 hasConceptScore W2089113485C64241487 @default.
• W2089113485 hasConceptScore W2089113485C67705224 @default.
• W2089113485 hasConceptScore W2089113485C82381507 @default.
• W2089113485 hasConceptScore W2089113485C86803240 @default.
• W2089113485 hasConceptScore W2089113485C88354546 @default.
• W2089113485 hasIssue "3" @default.
• W2089113485 hasLocation W20891134851 @default.
• W2089113485 hasLocation W20891134852 @default.
• W2089113485 hasOpenAccess W2089113485 @default.
• W2089113485 hasPrimaryLocation W20891134851 @default.
• W2089113485 hasRelatedWork W2011685655 @default.
• W2089113485 hasRelatedWork W2044794356 @default.
• W2089113485 hasRelatedWork W2045706660 @default.
• W2089113485 hasRelatedWork W2103113297 @default.
• W2089113485 hasRelatedWork W2161318049 @default.
• W2089113485 hasRelatedWork W2341116212 @default.
• W2089113485 hasRelatedWork W2411799562 @default.

• next