Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089127908> ?p ?o ?g. }
- W2089127908 endingPage "315" @default.
- W2089127908 startingPage "307" @default.
- W2089127908 abstract "Summary Arabidopsis thaliana abscisic acid insensitive 1‐1 ( abi1‐1 ) is a dominant mutant that is insensitive to the inhibition of germination and growth by the plant hormone, abscisic acid (ABA). The mutation severely decreases the catalytic activity of the ABI1 type 2C protein phosphatase (PP2C). However, the site of action of the abi1‐1 /ABI1 in the ABA signal transduction pathway has not yet been determined. Using single cell assays, we showed that microinjecting mutant abi1‐1 protein inhibited the activation of RD29A‐GUS and KIN2‐GUS in response to ABA, cyclic ADP‐ribose (cADPR), and Ca 2+ . The inhibitory effect of the mutant protein, however, was reversed by co‐microinjection of an excess amount of the ABI1 protein. In transgenic Arabidopsis plants, overexpression of abi1‐1 rendered the plants insensitive to ABA during germination, whereas overexpression of ABI1 did not have any apparent effect. Moreover, transgenic plants overexpressing abi1‐1 were blocked in the induction of ABA‐responsive genes; however, overexpression of ABI1 did not affect gene expression. Taken together, our results demonstrate that abi1‐1 is likely to be a dominant negative mutation and ABI1 likely acts downstream of cADPR in the ABA‐signaling pathway. Our results on ABI1 overexpression in Arabidopsis are not compatible with a negative regulatory role of this phosphatase in ABA responses." @default.
- W2089127908 created "2016-06-24" @default.
- W2089127908 creator A5009903480 @default.
- W2089127908 creator A5030506628 @default.
- W2089127908 creator A5032518248 @default.
- W2089127908 creator A5065168048 @default.
- W2089127908 creator A5066750408 @default.
- W2089127908 creator A5081271358 @default.
- W2089127908 date "2003-04-23" @default.
- W2089127908 modified "2023-10-17" @default.
- W2089127908 title "The <i>abi1‐1</i> mutation blocks ABA signaling downstream of cADPR action" @default.
- W2089127908 cites W1501460217 @default.
- W2089127908 cites W1576390601 @default.
- W2089127908 cites W1578218035 @default.
- W2089127908 cites W1833680771 @default.
- W2089127908 cites W1921320152 @default.
- W2089127908 cites W1975110054 @default.
- W2089127908 cites W1981218861 @default.
- W2089127908 cites W1986587865 @default.
- W2089127908 cites W1987808619 @default.
- W2089127908 cites W1993171576 @default.
- W2089127908 cites W2003406255 @default.
- W2089127908 cites W200946802 @default.
- W2089127908 cites W2012407846 @default.
- W2089127908 cites W2016716954 @default.
- W2089127908 cites W2016737807 @default.
- W2089127908 cites W2017942466 @default.
- W2089127908 cites W2019073197 @default.
- W2089127908 cites W2020607458 @default.
- W2089127908 cites W2025040786 @default.
- W2089127908 cites W2029436676 @default.
- W2089127908 cites W2030285455 @default.
- W2089127908 cites W2031126873 @default.
- W2089127908 cites W2032733531 @default.
- W2089127908 cites W2042377195 @default.
- W2089127908 cites W2049160993 @default.
- W2089127908 cites W2057567296 @default.
- W2089127908 cites W2059410839 @default.
- W2089127908 cites W2064258683 @default.
- W2089127908 cites W2091705630 @default.
- W2089127908 cites W2095295932 @default.
- W2089127908 cites W2106541825 @default.
- W2089127908 cites W2112259299 @default.
- W2089127908 cites W2129997424 @default.
- W2089127908 cites W2131525307 @default.
- W2089127908 cites W2132215587 @default.
- W2089127908 cites W2137054027 @default.
- W2089127908 cites W2142704168 @default.
- W2089127908 cites W2153096212 @default.
- W2089127908 cites W2164996942 @default.
- W2089127908 cites W4236384810 @default.
- W2089127908 cites W4376999337 @default.
- W2089127908 doi "https://doi.org/10.1046/j.1365-313x.2003.01721.x" @default.
- W2089127908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12713537" @default.
- W2089127908 hasPublicationYear "2003" @default.
- W2089127908 type Work @default.
- W2089127908 sameAs 2089127908 @default.
- W2089127908 citedByCount "73" @default.
- W2089127908 countsByYear W20891279082012 @default.
- W2089127908 countsByYear W20891279082013 @default.
- W2089127908 countsByYear W20891279082014 @default.
- W2089127908 countsByYear W20891279082016 @default.
- W2089127908 countsByYear W20891279082017 @default.
- W2089127908 countsByYear W20891279082018 @default.
- W2089127908 countsByYear W20891279082019 @default.
- W2089127908 countsByYear W20891279082020 @default.
- W2089127908 countsByYear W20891279082021 @default.
- W2089127908 countsByYear W20891279082023 @default.
- W2089127908 crossrefType "journal-article" @default.
- W2089127908 hasAuthorship W2089127908A5009903480 @default.
- W2089127908 hasAuthorship W2089127908A5030506628 @default.
- W2089127908 hasAuthorship W2089127908A5032518248 @default.
- W2089127908 hasAuthorship W2089127908A5065168048 @default.
- W2089127908 hasAuthorship W2089127908A5066750408 @default.
- W2089127908 hasAuthorship W2089127908A5081271358 @default.
- W2089127908 hasBestOaLocation W20891279081 @default.
- W2089127908 hasConcept C104317684 @default.
- W2089127908 hasConcept C143065580 @default.
- W2089127908 hasConcept C153911025 @default.
- W2089127908 hasConcept C2775988993 @default.
- W2089127908 hasConcept C2779491563 @default.
- W2089127908 hasConcept C2779591172 @default.
- W2089127908 hasConcept C55493867 @default.
- W2089127908 hasConcept C62478195 @default.
- W2089127908 hasConcept C86803240 @default.
- W2089127908 hasConcept C95444343 @default.
- W2089127908 hasConceptScore W2089127908C104317684 @default.
- W2089127908 hasConceptScore W2089127908C143065580 @default.
- W2089127908 hasConceptScore W2089127908C153911025 @default.
- W2089127908 hasConceptScore W2089127908C2775988993 @default.
- W2089127908 hasConceptScore W2089127908C2779491563 @default.
- W2089127908 hasConceptScore W2089127908C2779591172 @default.
- W2089127908 hasConceptScore W2089127908C55493867 @default.
- W2089127908 hasConceptScore W2089127908C62478195 @default.
- W2089127908 hasConceptScore W2089127908C86803240 @default.
- W2089127908 hasConceptScore W2089127908C95444343 @default.
- W2089127908 hasIssue "3" @default.
- W2089127908 hasLocation W20891279081 @default.