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• W2089202266 abstract "Abstract Ultrafiltration of goat pancreatic extract to obtain trypsin, using Soybean Trypsin Inhibitor as affinity ligand and a 30 kDa MWCO polyethersulfone membrane, is studied by examining the effect of varying transmembrane pressure and pancreatic extract: wash buffer ratio on active trypsin yield and flux/throughput profiles. For all process conditions considered, no detectable trypsin activity is found in the washing-phase permeates confirming excellent ligand-trypsin binding. Maximum trypsin yield obtained, at 1:1 feed ratio and 4 kg/cm2 pressure, is 74%. Mass flux of eluted protein as well as of washed-off impurities are also maximized under these conditions." @default.
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• W2089202266 date "2008-08-08" @default.
• W2089202266 modified "2023-09-23" @default.
• W2089202266 title "Affinity Separation of Trypsin from Goat Pancreatic Extract Using a Polyethersulfone Ultrafiltration Membrane" @default.
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• W2089202266 doi "https://doi.org/10.1080/01496390802215057" @default.
• W2089202266 hasPublicationYear "2008" @default.
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