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• W2089230617 abstract "Effect of calcium and hydrogen ion on the fibrinolytic activity of isolated renal glomeruli from rat. The fibrinolytic activity of glomeruli isolated from rat kidney has been measured with 125 I-fibrin-coated tubes. This technique allows the study of the two successive steps of the reaction: the release of the activator of plasminogen from the cells in the presence of isolated glomeruli, and the enzymatic process itself in the presence of the supernatant obtained after a 30-min incubation period with isolated glomeruli and centrifugation. Calcium in the range of 0.01 to 1.0mM increased the rate of fibrinolysis and shortened the lag-time preceding this effect. The fibrinolytic activity was also measured in the presence of isolated glomeruli as a function of the plasminogen concentration. Calcium at 1mM clearly increased the maximum velocity but did not change the concentration of plasminogen corresponding to 50% of the maximum. Supernatants were obtained after preincubation of isolated glomeruli with increasing doses of calcium. Addition of these supernatants to the 125 I-fibrin-coated tubes produced a progressive increase in the rate of fibrinolysis, depending on the dose of calcium. When calcium was added to the supernatants only, after having discarded the glomeruli, there was no change or, above 0.5mM calcium, a slight inhibition of the enzymatic reaction itself. The fibrinolytic activity measured in the presence of isolated glomeruli was maximum at a pH of 8. There was a clear-cut interaction in this condition between pH and calcium concentration. This interaction was not observed when the isolated glomeruli had been discarded. These results clearly demonstrate the major role of calcium in the release from glomeruli of an activator of plasminogen. Effet du calcium et de l'ion hydrogene sur l'activite fibrinolytique de glomerulus isoles de reins de rat. L'activite fibrinolytique de glomerules isoles de rein de rat a ete mesuree en utilisant des tubes sur lesquels de la 125 I fibrine a ete fixee. Cette technique permet d'apprecier les 2 etapes de la reaction: sortie de l'activateur cellulaire du plasminogene lorsque les glomerules isoles sont presents dans le milieu d'incubation et reaction enzymatique ellememe lorsque le surnageant obtenu apres 30min d'incubation et elimination des glomerules isoles par centrifugation est etudie. Le calcium entre 0.01 et 1.0mM augmente la quantite de fibrine lysee et raccourcit le temps de latence necessaire a cet effet. L'activite fibrinolytique a aussi ete mesuree en presence de glomerules isoles en fonction de la concentration de plasminogene. Le calcium a la concentration de 1mM augmente clairement la vitesse maximale mais ne change pas la concentration de plasminogene correspondant a 50% de l'effet maximum. Des surnageants ont ete obtenus apres pre incubation de glomerules isoles dans des milieux contenant des concentrations croissantes de calcium. L'adjonction de ceux-ci aux tubes sur lesquels de la 125 I fibrine a ete fixee entraine une augmentation croissante de la quantite de fibrine lysee dependante de la dose de calcium. Quand du calcium a ete ajoute a un surnageant obtenu apres avoir elimine les glomerules isoles il n'y a pas eu de modification de la fibrinolyse ou une legere inhibition de la reaction enzymatique pour des concentrations de calcium superieures a 0.5mM. L'activite fibrinolytique mesuree en presence de glomerules isoles a ete maximum a pH 8. Il y a eu une interaction claire entre le pH et la concentration de calcium qui n'a pas ete observee lorsque les glomerules ont ete elimines. Ces resultats demontrent clairement le role majeur du calcium dans la sortie des glomerules d'un activateur du plasminogene." @default.
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• W2089230617 date "1979-03-01" @default.
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• W2089230617 title "Effect of calcium and hydrogen ion on the fibrinolytic activity of isolated renal glomeruli from rat" @default.
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• W2089230617 doi "https://doi.org/10.1038/ki.1979.31" @default.
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