FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089238444> ?p ?o ?g. }

• W2089238444 endingPage "1260" @default.
• W2089238444 startingPage "1250" @default.
• W2089238444 abstract "Programmed frameshifting is one of the translational recoding mechanisms that read the genetic code in alternative ways. This process is generally programmed by signals at defined locations in a specific mRNA. In this study, we report the identification of hepta- and octo-uridine stretches as sole signals for programmed +1 and -1 ribosomal frameshifting during translation of severe acute respiratory syndrome coronavirus (SARS-CoV) ORF 3a variants. SARS-CoV ORF 3a encodes a minor structural protein of 274 amino acids. Over the course of cloning and expression of the gene, a mixed population of clones with six, seven, eight and nine T stretches located 14 nt downstream of the initiation codon was found. In vitro and in vivo expression of clones with six, seven and eight Ts, respectively, showed the detection of the full-length 3a protein. Mutagenesis studies led to the identification of the hepta- and octo-uridine stretches as slippery sequences for efficient frameshifting. Interestingly, no stimulatory elements were found in the sequences upstream or downstream of the slippage site. When the hepta- and octo-uridine stretches were used to replace the original slippery sequence of the SARS-CoV ORF 1a and 1b, efficient frameshift events were observed. Furthermore, the efficiencies of frameshifting mediated by the hepta- and octo-uridine stretches were not affected by mutations introduced into a downstream stem-loop structure that totally abolish the frameshift event mediated by the original slippery sequence of ORF 1a and 1b. Taken together, this study identifies the hepta- and octo-uridine stretches that function as sole elements for efficient +1 and -1 ribosomal frameshift events." @default.
• W2089238444 created "2016-06-24" @default.
• W2089238444 creator A5056006607 @default.
• W2089238444 date "2006-02-13" @default.
• W2089238444 modified "2023-09-30" @default.
• W2089238444 title "Identification of Hepta- and Octo-Uridine stretches as sole signals for programmed +1 and -1 ribosomal frameshifting during translation of SARS-CoV ORF 3a variants" @default.
• W2089238444 cites W1584410804 @default.
• W2089238444 cites W1606305585 @default.
• W2089238444 cites W1966108249 @default.
• W2089238444 cites W1988267408 @default.
• W2089238444 cites W1991657860 @default.
• W2089238444 cites W1999410197 @default.
• W2089238444 cites W2005067002 @default.
• W2089238444 cites W2015656039 @default.
• W2089238444 cites W2019054377 @default.
• W2089238444 cites W2029038593 @default.
• W2089238444 cites W2040843640 @default.
• W2089238444 cites W2044297195 @default.
• W2089238444 cites W2056873822 @default.
• W2089238444 cites W2061856039 @default.
• W2089238444 cites W2062407053 @default.
• W2089238444 cites W2064370916 @default.
• W2089238444 cites W2065088295 @default.
• W2089238444 cites W2066306545 @default.
• W2089238444 cites W2086958998 @default.
• W2089238444 cites W2089471930 @default.
• W2089238444 cites W2092974248 @default.
• W2089238444 cites W2107198142 @default.
• W2089238444 cites W2107534434 @default.
• W2089238444 cites W2113675613 @default.
• W2089238444 cites W2126911149 @default.
• W2089238444 cites W2149701165 @default.
• W2089238444 cites W2152482656 @default.
• W2089238444 cites W2168341495 @default.
• W2089238444 cites W3164064520 @default.
• W2089238444 doi "https://doi.org/10.1093/nar/gkl017" @default.
• W2089238444 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1383626" @default.
• W2089238444 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16500894" @default.
• W2089238444 hasPublicationYear "2006" @default.
• W2089238444 type Work @default.
• W2089238444 sameAs 2089238444 @default.
• W2089238444 citedByCount "7" @default.
• W2089238444 countsByYear W20892384442012 @default.
• W2089238444 countsByYear W20892384442014 @default.
• W2089238444 countsByYear W20892384442015 @default.
• W2089238444 crossrefType "journal-article" @default.
• W2089238444 hasAuthorship W2089238444A5056006607 @default.
• W2089238444 hasBestOaLocation W20892384441 @default.
• W2089238444 hasConcept C104317684 @default.
• W2089238444 hasConcept C105580179 @default.
• W2089238444 hasConcept C149364088 @default.
• W2089238444 hasConcept C153911025 @default.
• W2089238444 hasConcept C16318435 @default.
• W2089238444 hasConcept C166253671 @default.
• W2089238444 hasConcept C167625842 @default.
• W2089238444 hasConcept C20580545 @default.
• W2089238444 hasConcept C29906990 @default.
• W2089238444 hasConcept C47289529 @default.
• W2089238444 hasConcept C501734568 @default.
• W2089238444 hasConcept C54355233 @default.
• W2089238444 hasConcept C67705224 @default.
• W2089238444 hasConcept C86803240 @default.
• W2089238444 hasConcept C88478588 @default.
• W2089238444 hasConcept C95811502 @default.
• W2089238444 hasConceptScore W2089238444C104317684 @default.
• W2089238444 hasConceptScore W2089238444C105580179 @default.
• W2089238444 hasConceptScore W2089238444C149364088 @default.
• W2089238444 hasConceptScore W2089238444C153911025 @default.
• W2089238444 hasConceptScore W2089238444C16318435 @default.
• W2089238444 hasConceptScore W2089238444C166253671 @default.
• W2089238444 hasConceptScore W2089238444C167625842 @default.
• W2089238444 hasConceptScore W2089238444C20580545 @default.
• W2089238444 hasConceptScore W2089238444C29906990 @default.
• W2089238444 hasConceptScore W2089238444C47289529 @default.
• W2089238444 hasConceptScore W2089238444C501734568 @default.
• W2089238444 hasConceptScore W2089238444C54355233 @default.
• W2089238444 hasConceptScore W2089238444C67705224 @default.
• W2089238444 hasConceptScore W2089238444C86803240 @default.
• W2089238444 hasConceptScore W2089238444C88478588 @default.
• W2089238444 hasConceptScore W2089238444C95811502 @default.
• W2089238444 hasIssue "4" @default.
• W2089238444 hasLocation W20892384441 @default.
• W2089238444 hasLocation W20892384442 @default.
• W2089238444 hasLocation W20892384443 @default.
• W2089238444 hasLocation W20892384444 @default.
• W2089238444 hasOpenAccess W2089238444 @default.
• W2089238444 hasPrimaryLocation W20892384441 @default.
• W2089238444 hasRelatedWork W1574779369 @default.
• W2089238444 hasRelatedWork W1757123553 @default.
• W2089238444 hasRelatedWork W2008929657 @default.
• W2089238444 hasRelatedWork W2019389859 @default.
• W2089238444 hasRelatedWork W2033651440 @default.
• W2089238444 hasRelatedWork W2037676812 @default.
• W2089238444 hasRelatedWork W2089238444 @default.
• W2089238444 hasRelatedWork W2161583754 @default.
• W2089238444 hasRelatedWork W2285672361 @default.
• W2089238444 hasRelatedWork W2287942229 @default.
• W2089238444 hasVolume "34" @default.

• next