FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089308846> ?p ?o ?g. }

• W2089308846 endingPage "1952" @default.
• W2089308846 startingPage "1943" @default.
• W2089308846 abstract "Iberiotoxin (IbTX), a selective peptidyl ligand for high-conductance Ca2+-activated K+ (maxi-K) channels cannot be radioiodinated in biologically active form due to the importance of Y36 in interacting with the channel pore. Therefore, an IbTX double mutant (IbTX-D19Y/Y36F) was engineered, expressed in Escherichia coli, purified to homogeneity, and radiolabeled to high specific activity with 125I. IbTX-D19Y/Y36F and [127I]IbTX-D19Y/Y36F block maxi-K channels expressed in Xenopus laevis oocytes with equal potency as wild-type IbTX (Kd ∼ 1 nM). Under low ionic strength conditions, [125I]IbTX-D19Y/Y36F binds with high affinity to smooth muscle sarcolemmal maxi-K channels (Kd of 5 pM, as determined by either equilibrium binding or kinetic binding analysis), and with a binding site density of 0.45 pmol/mg of protein. Competition studies with wild-type IbTX, IbTX-D19Y/Y36F or charybdotoxin (ChTX) result in complete inhibition of binding whereas toxins selective for voltage-gated K+ channels (margatoxin (MgTX) or α-dendrotoxin (α-DaTX)) do not have any effect on IbTX binding. Indole diterpene alkaloids, which are selective inhibitors of maxi-K channels, and potassium ions both modulate [125I]IbTX-D19Y/Y36F binding in a complex manner. This pattern is also reflected during covalent incorporation of the radiolabeled toxin into the 31 kDa β-subunit of the maxi-K channel in the presence of a bifunctional cross-linking reagent. In rat brain membranes, IbTX-D19Y/Y36F does not displace binding of [125I]MgTX or [125I]-α-DaTX to sites associated with voltage-gated K+ channels, nor do these latter toxins inhibit [125I]IbTX-D19Y/Y36F binding. Taken together, these results demonstrate that [125I]IbTX-D19Y/Y36F is the first selective radioligand for maxi-K channels with high specific activity." @default.
• W2089308846 created "2016-06-24" @default.
• W2089308846 creator A5001718162 @default.
• W2089308846 creator A5026860985 @default.
• W2089308846 creator A5042514932 @default.
• W2089308846 creator A5060818175 @default.
• W2089308846 creator A5063480942 @default.
• W2089308846 creator A5066666154 @default.
• W2089308846 creator A5090778583 @default.
• W2089308846 date "1997-02-01" @default.
• W2089308846 modified "2023-10-18" @default.
• W2089308846 title "[¹²⁵I]Iberiotoxin-D19Y/Y36F, the First Selective, High Specific Activity Radioligand for High-Conductance Calcium-Activated Potassium Channels" @default.
• W2089308846 cites W1494926748 @default.
• W2089308846 cites W1555733423 @default.
• W2089308846 cites W1558096813 @default.
• W2089308846 cites W1575653277 @default.
• W2089308846 cites W1602607306 @default.
• W2089308846 cites W1626920804 @default.
• W2089308846 cites W1854945595 @default.
• W2089308846 cites W1974368361 @default.
• W2089308846 cites W1976466808 @default.
• W2089308846 cites W1992496710 @default.
• W2089308846 cites W2014392093 @default.
• W2089308846 cites W2021958245 @default.
• W2089308846 cites W2104404316 @default.
• W2089308846 cites W2114838789 @default.
• W2089308846 cites W2131573781 @default.
• W2089308846 cites W2145100019 @default.
• W2089308846 doi "https://doi.org/10.1021/bi962074m" @default.
• W2089308846 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9048582" @default.
• W2089308846 hasPublicationYear "1997" @default.
• W2089308846 type Work @default.
• W2089308846 sameAs 2089308846 @default.
• W2089308846 citedByCount "43" @default.
• W2089308846 countsByYear W20893088462012 @default.
• W2089308846 countsByYear W20893088462013 @default.
• W2089308846 countsByYear W20893088462014 @default.
• W2089308846 countsByYear W20893088462017 @default.
• W2089308846 countsByYear W20893088462018 @default.
• W2089308846 countsByYear W20893088462019 @default.
• W2089308846 countsByYear W20893088462020 @default.
• W2089308846 countsByYear W20893088462023 @default.
• W2089308846 crossrefType "journal-article" @default.
• W2089308846 hasAuthorship W2089308846A5001718162 @default.
• W2089308846 hasAuthorship W2089308846A5026860985 @default.
• W2089308846 hasAuthorship W2089308846A5042514932 @default.
• W2089308846 hasAuthorship W2089308846A5060818175 @default.
• W2089308846 hasAuthorship W2089308846A5063480942 @default.
• W2089308846 hasAuthorship W2089308846A5066666154 @default.
• W2089308846 hasAuthorship W2089308846A5090778583 @default.
• W2089308846 hasConcept C12554922 @default.
• W2089308846 hasConcept C185592680 @default.
• W2089308846 hasConcept C2779411790 @default.
• W2089308846 hasConcept C2780419564 @default.
• W2089308846 hasConcept C55493867 @default.
• W2089308846 hasConcept C83743174 @default.
• W2089308846 hasConcept C86803240 @default.
• W2089308846 hasConceptScore W2089308846C12554922 @default.
• W2089308846 hasConceptScore W2089308846C185592680 @default.
• W2089308846 hasConceptScore W2089308846C2779411790 @default.
• W2089308846 hasConceptScore W2089308846C2780419564 @default.
• W2089308846 hasConceptScore W2089308846C55493867 @default.
• W2089308846 hasConceptScore W2089308846C83743174 @default.
• W2089308846 hasConceptScore W2089308846C86803240 @default.
• W2089308846 hasIssue "7" @default.
• W2089308846 hasLocation W20893088461 @default.
• W2089308846 hasLocation W20893088462 @default.
• W2089308846 hasOpenAccess W2089308846 @default.
• W2089308846 hasPrimaryLocation W20893088461 @default.
• W2089308846 hasRelatedWork W2002591410 @default.
• W2089308846 hasRelatedWork W2004883803 @default.
• W2089308846 hasRelatedWork W2016247671 @default.
• W2089308846 hasRelatedWork W2029070759 @default.
• W2089308846 hasRelatedWork W2045400413 @default.
• W2089308846 hasRelatedWork W2088281397 @default.
• W2089308846 hasRelatedWork W2126681935 @default.
• W2089308846 hasRelatedWork W2161661722 @default.
• W2089308846 hasRelatedWork W2312672762 @default.
• W2089308846 hasRelatedWork W2418008882 @default.
• W2089308846 hasVolume "36" @default.
• W2089308846 isParatext "false" @default.
• W2089308846 isRetracted "false" @default.
• W2089308846 magId "2089308846" @default.
• W2089308846 workType "article" @default.