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- W2089318676 abstract "By using carbon monoxide as a reagent for releasing oxygen from blood solutions (instead of the customary potassium ferricyanide) and by combining the techniques of the Van Slyke constant-volume gasometric apparatus and the Scholander gas analysis apparatus, a precise method has been devised of estimating oxygen content and carbon monoxide capacity simultaneously on one and the same sample of blood solution. The procedure eliminates various errors inherent in the determination of oxygen percentage saturation, when oxygen content and capacity are successively measured on separate samples. The method has been extensively tested on sheep blood solutions containing 3 to 4 g haemoglobin/100 ml. and is considered to give an accuracy of ± 0.5 in the oxygen percentage saturation of such solutions. In its present form the method is only applicable to solutions containing a negligible amount of dissolved nitrogen, but it is probable that this restriction could be removed by the simple addition to the technique suggested in the text. Modifications are also described in the usual tonometer technique for equilibrating blood solutions with various pressures of oxygen, and for transferring the equilibrated blood solution to the Van Slyke apparatus for analysis. The techniques of the present paper have been specially used for obtaining oxyhaemoglobin dissociation curve data of higher accuracy than hitherto available. From these improved data it has for the first time been possible to calculate directly, and in many cases with fair precision, the values o f the equilibrium constants of the four successive reactions of haemoglobin with oxygen." @default.
- W2089318676 created "2016-06-24" @default.
- W2089318676 date "1955-08-16" @default.
- W2089318676 modified "2023-09-24" @default.
- W2089318676 title "Improved gasometric methods for estimating oxygen content and percentage saturation in haemoglobin dissociation curve determinations" @default.
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- W2089318676 doi "https://doi.org/10.1098/rspb.1955.0033" @default.
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