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- W2089387484 abstract "A detergent scrub technique using wash fluid consisting of 0·075 M phosphate-buffered saline, pH 7·9 containing 0·1 per cent Triton X-100 was evaluated for the quantitative culture of Malassezia pachydermatis from canine skin. Preliminary studies showed that the detergents Triton X-100, Tween 40 and Tween 80 were equally able to disperse suspensions of pure cultures of M pachydermatis, but that the yeast counts were significantly reduced (P<0·001) after suspension in saline, Triton X-100 or Tween 40 for two hours. The counts in skin washings were also reduced (P<0·001) after suspension for three hours in 0·1 and 0·05 per cent solutions of Triton X-100. Vortexing, or manual or mechanical shaking of the samples yielded comparable counts. The correlation between the counts on diseased skin measured by using detergent scrubs and a contact plate technique was highly significant (P<0·001). The detergent scrub technique was suitable for the quantitation of M pachydermatis on canine skin provided that the samples were processed without delay." @default.
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- W2089387484 date "1995-03-01" @default.
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- W2089387484 title "Evaluation of a detergent scrub technique for the quantitative culture of Malassezia pachydermatis from canine skin" @default.
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- W2089387484 doi "https://doi.org/10.1016/0034-5288(95)90066-7" @default.
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