Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089489735> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2089489735 endingPage "939" @default.
- W2089489735 startingPage "931" @default.
- W2089489735 abstract "Anti-dsDNA autoantibodies and immune complex formation are major factors in SLE pathogenesis. Understanding stable immune complex formation is critical in deciphering mechanisms of autoimmune pathogenesis. Previous studies identified a subpopulation of murine lupus monoclonal autoantibodies that exhibited dual specificity (anti-DNA and anti-IgG2a hinge) and formed stable immune complexes [J. Mol. Rec. 10(1997)225]. Two monoclonal autoantibodies, BV 17-45 and BV 16-13, were extensively studied because of their dual specificity. To quantitatively assess the role of each specificity in the formation of stable immune complexes, studies were performed to determine binding affinities for various sized dsDNA fragments (21, 43, 84, and 114 bp) and the covalent dimer of a nine amino acid hinge peptide. Results characterizing BV 17-45 showed that the affinity for dsDNA directly correlated with increased dsDNA size. Results with BV 16-13 revealed a generally lower affinity for the various dsDNA fragments. Binding inhibition studies, using a covalently linked dimer of a nine amino acid synthetic hinge peptide as an inhibitor of the antibody-43 bp dsDNA interaction, yielded relative affinities for the anti-hinge activity. Binding affinities for the synthetic hinge specificity were lower than affinities measured for the anti-dsDNA activity. Collectively, the binding and inhibition studies provided insight into the correlation between dual specificity and avid immune complex formation. A model was proposed based on the concept that large dsDNA fragments caused localization of the dual-specific antibodies through the anti-dsDNA activity, thereby facilitating subsequent binding and cross-linkage via the anti-hinge specificity. These synergistic interactions resulted in the formation of avid immune complexes." @default.
- W2089489735 created "2016-06-24" @default.
- W2089489735 creator A5008106149 @default.
- W2089489735 creator A5052276163 @default.
- W2089489735 date "2000-10-01" @default.
- W2089489735 modified "2023-09-25" @default.
- W2089489735 title "Interaction of dual-specific autoantibodies with dsDNA and a synthetic dimer peptide simulating the hinge region of IgG2a molecules" @default.
- W2089489735 cites W1552738427 @default.
- W2089489735 cites W1565145184 @default.
- W2089489735 cites W1573008071 @default.
- W2089489735 cites W160997634 @default.
- W2089489735 cites W1967815894 @default.
- W2089489735 cites W1990149261 @default.
- W2089489735 cites W2001977423 @default.
- W2089489735 cites W2002272420 @default.
- W2089489735 cites W2034880487 @default.
- W2089489735 cites W2037727534 @default.
- W2089489735 cites W2046861730 @default.
- W2089489735 cites W2052721541 @default.
- W2089489735 cites W2054080837 @default.
- W2089489735 cites W2056920290 @default.
- W2089489735 cites W2073536631 @default.
- W2089489735 cites W2075365477 @default.
- W2089489735 cites W2082967009 @default.
- W2089489735 cites W2099513954 @default.
- W2089489735 cites W2133786706 @default.
- W2089489735 cites W2142360671 @default.
- W2089489735 cites W2227157639 @default.
- W2089489735 doi "https://doi.org/10.1016/s0161-5890(00)00108-5" @default.
- W2089489735 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11282396" @default.
- W2089489735 hasPublicationYear "2000" @default.
- W2089489735 type Work @default.
- W2089489735 sameAs 2089489735 @default.
- W2089489735 citedByCount "0" @default.
- W2089489735 crossrefType "journal-article" @default.
- W2089489735 hasAuthorship W2089489735A5008106149 @default.
- W2089489735 hasAuthorship W2089489735A5052276163 @default.
- W2089489735 hasConcept C153911025 @default.
- W2089489735 hasConcept C159654299 @default.
- W2089489735 hasConcept C163764329 @default.
- W2089489735 hasConcept C178790620 @default.
- W2089489735 hasConcept C185592680 @default.
- W2089489735 hasConcept C203014093 @default.
- W2089489735 hasConcept C2778474638 @default.
- W2089489735 hasConcept C2779281246 @default.
- W2089489735 hasConcept C2779546866 @default.
- W2089489735 hasConcept C2780283098 @default.
- W2089489735 hasConcept C515207424 @default.
- W2089489735 hasConcept C542903549 @default.
- W2089489735 hasConcept C55493867 @default.
- W2089489735 hasConcept C86803240 @default.
- W2089489735 hasConcept C8891405 @default.
- W2089489735 hasConceptScore W2089489735C153911025 @default.
- W2089489735 hasConceptScore W2089489735C159654299 @default.
- W2089489735 hasConceptScore W2089489735C163764329 @default.
- W2089489735 hasConceptScore W2089489735C178790620 @default.
- W2089489735 hasConceptScore W2089489735C185592680 @default.
- W2089489735 hasConceptScore W2089489735C203014093 @default.
- W2089489735 hasConceptScore W2089489735C2778474638 @default.
- W2089489735 hasConceptScore W2089489735C2779281246 @default.
- W2089489735 hasConceptScore W2089489735C2779546866 @default.
- W2089489735 hasConceptScore W2089489735C2780283098 @default.
- W2089489735 hasConceptScore W2089489735C515207424 @default.
- W2089489735 hasConceptScore W2089489735C542903549 @default.
- W2089489735 hasConceptScore W2089489735C55493867 @default.
- W2089489735 hasConceptScore W2089489735C86803240 @default.
- W2089489735 hasConceptScore W2089489735C8891405 @default.
- W2089489735 hasFunder F4320306230 @default.
- W2089489735 hasIssue "15" @default.
- W2089489735 hasLocation W20894897351 @default.
- W2089489735 hasLocation W20894897352 @default.
- W2089489735 hasOpenAccess W2089489735 @default.
- W2089489735 hasPrimaryLocation W20894897351 @default.
- W2089489735 hasRelatedWork W16729089 @default.
- W2089489735 hasRelatedWork W1973692620 @default.
- W2089489735 hasRelatedWork W1987381631 @default.
- W2089489735 hasRelatedWork W1992169430 @default.
- W2089489735 hasRelatedWork W2009889818 @default.
- W2089489735 hasRelatedWork W2013489706 @default.
- W2089489735 hasRelatedWork W2038126255 @default.
- W2089489735 hasRelatedWork W2102639438 @default.
- W2089489735 hasRelatedWork W2368460920 @default.
- W2089489735 hasRelatedWork W2380360044 @default.
- W2089489735 hasVolume "37" @default.
- W2089489735 isParatext "false" @default.
- W2089489735 isRetracted "false" @default.
- W2089489735 magId "2089489735" @default.
- W2089489735 workType "article" @default.