FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089492028> ?p ?o ?g. }

• W2089492028 endingPage "3196" @default.
• W2089492028 startingPage "3191" @default.
• W2089492028 abstract "Biologically produced alkanes represent potential renewable alternatives to petroleum-derived chemicals. A cyanobacterial pathway consisting of acyl–Acyl Carrier Protein reductase and an aldehyde-deformylating oxygenase (ADO) converts acyl–Acyl Carrier Proteins into corresponding n-1 alkanes via aldehyde intermediates in an oxygen-dependent manner (K m for O 2 , 84 ± 9 µM). In vitro, ADO turned over only three times, but addition of more ADO to exhausted assays resulted in additional product formation. While evaluating the peroxide shunt to drive ADO catalysis, we discovered that ADO is inhibited by hydrogen peroxide (H 2 O 2 ) with an apparent K i of 16 ± 6 µM and that H 2 O 2 inhibition is of mixed-type with respect to O 2 . Supplementing exhausted assays with catalase (CAT) restored ADO activity, demonstrating that inhibition was reversible and dependent on H 2 O 2 , which originated from poor coupling of reductant consumption with alkane formation. Kinetic analysis showed that long-chain (C14–C18) substrates follow Michaelis–Menten kinetics, whereas short and medium chains (C8–C12) exhibit substrate inhibition. A bifunctional protein comprising an N-terminal CAT coupled to a C-terminal ADO (CAT–ADO) prevents H 2 O 2 inhibition by converting it to the cosubstrate O 2 . Indeed, alkane production by the fusion protein is observed upon addition of H 2 O 2 to an anaerobic reaction mix. In assays, CAT–ADO turns over 225 times versus three times for the native ADO, and its expression in Escherichia coli increases catalytic turnovers per active site by fivefold relative to the expression of native ADO. We propose the term “protection via inhibitor metabolism” for fusion proteins designed to metabolize inhibitors into noninhibitory compounds." @default.
• W2089492028 created "2016-06-24" @default.
• W2089492028 creator A5013044807 @default.
• W2089492028 creator A5032984193 @default.
• W2089492028 creator A5041862198 @default.
• W2089492028 creator A5068584320 @default.
• W2089492028 date "2013-02-07" @default.
• W2089492028 modified "2023-10-16" @default.
• W2089492028 title "Fusing catalase to an alkane-producing enzyme maintains enzymatic activity by converting the inhibitory byproduct H ₂ O ₂ to the cosubstrate O ₂" @default.
• W2089492028 cites W1577685550 @default.
• W2089492028 cites W1593306904 @default.
• W2089492028 cites W1834249524 @default.
• W2089492028 cites W1976274445 @default.
• W2089492028 cites W1991324143 @default.
• W2089492028 cites W2024154446 @default.
• W2089492028 cites W2044388851 @default.
• W2089492028 cites W2044952220 @default.
• W2089492028 cites W2052504986 @default.
• W2089492028 cites W2053467255 @default.
• W2089492028 cites W2055544854 @default.
• W2089492028 cites W2061342207 @default.
• W2089492028 cites W2079636531 @default.
• W2089492028 cites W2095536791 @default.
• W2089492028 cites W2105608546 @default.
• W2089492028 cites W2119486813 @default.
• W2089492028 cites W2145164353 @default.
• W2089492028 cites W2147516046 @default.
• W2089492028 cites W2149859312 @default.
• W2089492028 cites W2150728030 @default.
• W2089492028 cites W2164098890 @default.
• W2089492028 cites W2165744022 @default.
• W2089492028 cites W2323578482 @default.
• W2089492028 cites W2598177557 @default.
• W2089492028 doi "https://doi.org/10.1073/pnas.1218769110" @default.
• W2089492028 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3581945" @default.
• W2089492028 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23391732" @default.
• W2089492028 hasPublicationYear "2013" @default.
• W2089492028 type Work @default.
• W2089492028 sameAs 2089492028 @default.
• W2089492028 citedByCount "110" @default.
• W2089492028 countsByYear W20894920282013 @default.
• W2089492028 countsByYear W20894920282014 @default.
• W2089492028 countsByYear W20894920282015 @default.
• W2089492028 countsByYear W20894920282016 @default.
• W2089492028 countsByYear W20894920282017 @default.
• W2089492028 countsByYear W20894920282018 @default.
• W2089492028 countsByYear W20894920282019 @default.
• W2089492028 countsByYear W20894920282020 @default.
• W2089492028 countsByYear W20894920282021 @default.
• W2089492028 countsByYear W20894920282022 @default.
• W2089492028 countsByYear W20894920282023 @default.
• W2089492028 crossrefType "journal-article" @default.
• W2089492028 hasAuthorship W2089492028A5013044807 @default.
• W2089492028 hasAuthorship W2089492028A5032984193 @default.
• W2089492028 hasAuthorship W2089492028A5041862198 @default.
• W2089492028 hasAuthorship W2089492028A5068584320 @default.
• W2089492028 hasBestOaLocation W20894920281 @default.
• W2089492028 hasConcept C161790260 @default.
• W2089492028 hasConcept C181199279 @default.
• W2089492028 hasConcept C185592680 @default.
• W2089492028 hasConcept C18903297 @default.
• W2089492028 hasConcept C197957613 @default.
• W2089492028 hasConcept C2777289219 @default.
• W2089492028 hasConcept C2778979269 @default.
• W2089492028 hasConcept C2779825165 @default.
• W2089492028 hasConcept C2780014933 @default.
• W2089492028 hasConcept C2781327020 @default.
• W2089492028 hasConcept C533411734 @default.
• W2089492028 hasConcept C55493867 @default.
• W2089492028 hasConcept C71240020 @default.
• W2089492028 hasConcept C73969872 @default.
• W2089492028 hasConcept C86803240 @default.
• W2089492028 hasConceptScore W2089492028C161790260 @default.
• W2089492028 hasConceptScore W2089492028C181199279 @default.
• W2089492028 hasConceptScore W2089492028C185592680 @default.
• W2089492028 hasConceptScore W2089492028C18903297 @default.
• W2089492028 hasConceptScore W2089492028C197957613 @default.
• W2089492028 hasConceptScore W2089492028C2777289219 @default.
• W2089492028 hasConceptScore W2089492028C2778979269 @default.
• W2089492028 hasConceptScore W2089492028C2779825165 @default.
• W2089492028 hasConceptScore W2089492028C2780014933 @default.
• W2089492028 hasConceptScore W2089492028C2781327020 @default.
• W2089492028 hasConceptScore W2089492028C533411734 @default.
• W2089492028 hasConceptScore W2089492028C55493867 @default.
• W2089492028 hasConceptScore W2089492028C71240020 @default.
• W2089492028 hasConceptScore W2089492028C73969872 @default.
• W2089492028 hasConceptScore W2089492028C86803240 @default.
• W2089492028 hasIssue "8" @default.
• W2089492028 hasLocation W20894920281 @default.
• W2089492028 hasLocation W20894920282 @default.
• W2089492028 hasLocation W20894920283 @default.
• W2089492028 hasLocation W20894920284 @default.
• W2089492028 hasOpenAccess W2089492028 @default.
• W2089492028 hasPrimaryLocation W20894920281 @default.
• W2089492028 hasRelatedWork W1487888634 @default.
• W2089492028 hasRelatedWork W1500231362 @default.
• W2089492028 hasRelatedWork W1707455315 @default.
• W2089492028 hasRelatedWork W1967232283 @default.

• next