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- W2089596961 abstract "A novel method for the detection of specific microbes using the polymerase chain reaction (PCR) technique was developed and applied for the quantitative detection of Enterobacter cloacae strain HO-1 (HO-1, hereafter) in the experimental apparatus used for the treatment of wastewater containing hexavalent chromium (chromate). Note that HO-1 is capable of reducing toxic chromate to trivalent chromium. Based upon the preliminary experiments using pUC18 DNA, conditions giving a constant amplification rate of the targeted DNA with high reliability were determined for the quantitative detection of its initial concentration by the extrapolation. The cell density of HO-1 determined by the PCR technique was compared with the results counted on the selective medium agar plate conventionally used for the counting of HO-1. The procedure proposed here was successfully applied to estimate the cell density of Enterobacter cloacae HO-1 in the bioreactor for the treatment of chromate wastewater." @default.
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- W2089596961 date "1998-11-01" @default.
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- W2089596961 title "Quantitative detection of Enterobacter cloacae strain HO-1 in bioreactor for chromate wastewater treatment using polymerase chain reaction (PCR)" @default.
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- W2089596961 doi "https://doi.org/10.1016/s0043-1354(98)00106-7" @default.
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