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• W2089672068 endingPage "4150" @default.
• W2089672068 startingPage "4150" @default.
• W2089672068 abstract "To explore the possibility of using the Non-invasive Micro-test Technique (NMT) to investigate the role of Transient Receptor Potential Canonical 1 (TRPC1) in regulating Ca(2+) influxes in HL-7702 cells, a normal human liver cell line.Net Ca(2+) fluxes were measured with NMT, a technology that can obtain dynamic information of specific/selective ionic/molecular activities on material surfaces, non-invasively. The expression levels of TRPC1 were increased by liposomal transfection, whose effectiveness was evaluated by Western-blotting and single cell reverse transcription-polymerase chain reaction.Ca(2+) influxes could be elicited by adding 1 mmol/L CaCl(2) to the test solution of HL-7702 cells. They were enhanced by addition of 20 micromol/L noradrenaline and inhibited by 100 micromol/L LaCl(3) (a non-selective Ca(2+) channel blocker); 5 micromol/L nifedipine did not induce any change. Overexpression of TRPC1 caused increased Ca(2+) influx. Five micromoles per liter nifedipine did not inhibit this elevation, whereas 100 micromol/L LaCl(3) did.In HL-7702 cells, there is a type of TRPC1-dependent Ca(2+) channel, which could be detected via NMT and inhibited by La(3+)." @default.
• W2089672068 created "2016-06-24" @default.
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• W2089672068 date "2009-01-01" @default.
• W2089672068 modified "2023-09-26" @default.
• W2089672068 title "Measuring Ca²⁺influxes of TRPC1-dependent Ca²⁺channels in HL-7702 cells with Non-invasive Micro-test Technique" @default.
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• W2089672068 doi "https://doi.org/10.3748/wjg.15.4150" @default.
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