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- W2089683374 abstract "The lipid composition and lipid metabolism of protoplasts isolated from cucumber (Cucumis sativus) cotyledons were examined. Comparisons were made of the fresh tissue, tissue which had been incubated in the protoplasting medium without the digestive enzymes, the freshly prepared protoplasts and protoplasts conditioned by storage for 8 hr at 27°. Triacylglycerols (25 %), monogalactosyldiacylglycerols (38 %) and phosphatidylcholine (21 %) accounted for most of the lipid in the fresh tissue. In the freshly prepared protoplasts these percentages were, respectively, 51, 21 and 8. The fatty acid compositions of these lipids were somewhat changed in the freshly prepared protoplasts. There were decreases in 18:3 in phosphatidylglycerol and phosphatidylethanolamine, and decreases in 18:2 in digalactosyldiacylglycerol and phosphatidylcholine. Incorporation of 14C-acetate for 1 hr in the fresh tissue was mainly into phosphatidylglycerol (22.2%), phosphatidylcholine (19.2%) and triacylglycerols+diacylglyc erols (14.5%). In freshly isolated protoplasts these figures were 16.5, 43.2 and 21.5%, r of 14C-bicarbonate for 1 hr was 22.5% into triacylglycerols+diacylglycerols 3.7% into phosphatidylglycerol and 5. 5% into phosphatidylcholine in the fresh tissue. In freshly prepared protoplasts the values were 25.6, 12.4 and 22.5%, respectively. Changes in the lipid composition and metabolism of conditioned protoplasts compared to freshly prepared protoplasts, which are more sensitive to chilling, did not suggest any relationship to chilling injury." @default.
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- W2089683374 date "1993-11-01" @default.
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- W2089683374 title "Lipid composition and biosynthesis in protoplasts from cucumber cotyledons" @default.
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- W2089683374 doi "https://doi.org/10.1016/s0031-9422(00)90705-2" @default.
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