Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089700871> ?p ?o ?g. }
- W2089700871 endingPage "132" @default.
- W2089700871 startingPage "122" @default.
- W2089700871 abstract "The detection and characterization of the hybridization event of 21-base, unlabeled DNA oligonucleotides with a monolayer of complementary DNA immobilized on a gold surface, by electrochemical impedance spectroscopy and surface plasmon resonance (SPR) is presented. A thiol modification on the probe DNA strand allowed for its attachment to the surface via self-assembly. For the hybridization of full match target DNA a detection limit of 20 pM was determined. RNA hybridization was also detectable with the same sensor, with a similar detection limit. The SPR signal generated upon hybridization of the full match was always distinguishable from the single mismatch target DNA oligonucleotides when the mismatch was in the middle or at the proximal end of the target DNA sequence. However, the response of the sensor was identical for the hybridization of the full match and the distal end mismatch. The SPR sensor described is reusable over at least 20 hybridization/regeneration cycles and is insensitive to flow rate (20-800 µL min-1) or temperature (20-60 °C). Based on the SPR response, the surface density of the probe was estimated to be at least 4.3 × 1012 molecules per cm2." @default.
- W2089700871 created "2016-06-24" @default.
- W2089700871 creator A5001160114 @default.
- W2089700871 creator A5032451762 @default.
- W2089700871 creator A5046958491 @default.
- W2089700871 creator A5050897448 @default.
- W2089700871 creator A5057543954 @default.
- W2089700871 date "2011-01-01" @default.
- W2089700871 modified "2023-10-18" @default.
- W2089700871 title "SPR-based single nucleotide mismatch biosensor" @default.
- W2089700871 cites W1503370327 @default.
- W2089700871 cites W1964353947 @default.
- W2089700871 cites W1974648751 @default.
- W2089700871 cites W1975304818 @default.
- W2089700871 cites W1976378503 @default.
- W2089700871 cites W1987312342 @default.
- W2089700871 cites W1988646878 @default.
- W2089700871 cites W2001470716 @default.
- W2089700871 cites W2004391976 @default.
- W2089700871 cites W2004646294 @default.
- W2089700871 cites W2005753585 @default.
- W2089700871 cites W2010794685 @default.
- W2089700871 cites W2014438788 @default.
- W2089700871 cites W2014567256 @default.
- W2089700871 cites W2018136308 @default.
- W2089700871 cites W2027860183 @default.
- W2089700871 cites W2030552522 @default.
- W2089700871 cites W2035068690 @default.
- W2089700871 cites W2036758819 @default.
- W2089700871 cites W2039078882 @default.
- W2089700871 cites W2040657148 @default.
- W2089700871 cites W2043468628 @default.
- W2089700871 cites W2048702576 @default.
- W2089700871 cites W2048920219 @default.
- W2089700871 cites W2050546635 @default.
- W2089700871 cites W2052219628 @default.
- W2089700871 cites W2054386813 @default.
- W2089700871 cites W2055996804 @default.
- W2089700871 cites W2057833839 @default.
- W2089700871 cites W2058967883 @default.
- W2089700871 cites W2062752859 @default.
- W2089700871 cites W2065187211 @default.
- W2089700871 cites W2069989335 @default.
- W2089700871 cites W2071295799 @default.
- W2089700871 cites W2079821592 @default.
- W2089700871 cites W2080177429 @default.
- W2089700871 cites W2082277797 @default.
- W2089700871 cites W2087546439 @default.
- W2089700871 cites W2090791561 @default.
- W2089700871 cites W2092138304 @default.
- W2089700871 cites W2095177911 @default.
- W2089700871 cites W2097025265 @default.
- W2089700871 cites W2109368873 @default.
- W2089700871 cites W2112219727 @default.
- W2089700871 cites W2120686074 @default.
- W2089700871 cites W2120890726 @default.
- W2089700871 cites W2126012074 @default.
- W2089700871 cites W2138664009 @default.
- W2089700871 cites W2145572269 @default.
- W2089700871 cites W2149739339 @default.
- W2089700871 cites W2154841861 @default.
- W2089700871 cites W2155784836 @default.
- W2089700871 cites W2166806803 @default.
- W2089700871 cites W2167442883 @default.
- W2089700871 cites W4298158007 @default.
- W2089700871 cites W95436083 @default.
- W2089700871 doi "https://doi.org/10.1039/c0ay00492h" @default.
- W2089700871 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/32938121" @default.
- W2089700871 hasPublicationYear "2011" @default.
- W2089700871 type Work @default.
- W2089700871 sameAs 2089700871 @default.
- W2089700871 citedByCount "13" @default.
- W2089700871 countsByYear W20897008712013 @default.
- W2089700871 countsByYear W20897008712014 @default.
- W2089700871 countsByYear W20897008712015 @default.
- W2089700871 countsByYear W20897008712016 @default.
- W2089700871 countsByYear W20897008712017 @default.
- W2089700871 countsByYear W20897008712019 @default.
- W2089700871 countsByYear W20897008712020 @default.
- W2089700871 countsByYear W20897008712021 @default.
- W2089700871 countsByYear W20897008712023 @default.
- W2089700871 crossrefType "journal-article" @default.
- W2089700871 hasAuthorship W2089700871A5001160114 @default.
- W2089700871 hasAuthorship W2089700871A5032451762 @default.
- W2089700871 hasAuthorship W2089700871A5046958491 @default.
- W2089700871 hasAuthorship W2089700871A5050897448 @default.
- W2089700871 hasAuthorship W2089700871A5057543954 @default.
- W2089700871 hasConcept C106847996 @default.
- W2089700871 hasConcept C113196181 @default.
- W2089700871 hasConcept C119128265 @default.
- W2089700871 hasConcept C129312508 @default.
- W2089700871 hasConcept C130197536 @default.
- W2089700871 hasConcept C137390916 @default.
- W2089700871 hasConcept C155672457 @default.
- W2089700871 hasConcept C160756335 @default.
- W2089700871 hasConcept C171250308 @default.
- W2089700871 hasConcept C185592680 @default.
- W2089700871 hasConcept C192562407 @default.