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- W2089742015 abstract "Diethylpyrocarbonate (DEP) in the pH range 6.1 – 7.5 inactivates thermolysin by ethoxyformylation. Restoration of activity by hydroxylamine at pH 6.2 correlates with the regeneration of a single histidyl residue. Exposure of the enzyme to DEP together with the reversible inhibitor β-phenylpropionyl-L-phenylalanine, or acylation with the mixed anhydride of β-phenylpropionyl-L-phenylalanine and ethoxyformic acid or with β-phenylpropionyl-L-phenylalanyl-imidazole increases the activity by an order of magnitude toward both peptide and ester substrates. Treatment with NH2OH restores the catalytic properties of this superactive derivative to that of the native enzyme while modification with DEP destroys activity completely." @default.
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- W2089742015 date "1973-04-01" @default.
- W2089742015 modified "2023-10-16" @default.
- W2089742015 title "Reversible inactivation and superactivation by covalent modification of thermolysin" @default.
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- W2089742015 doi "https://doi.org/10.1016/0006-291x(73)90024-7" @default.
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