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• W2089781348 abstract "ObjectiveLaser-assisted techniques facilitate the procedure of embryo biopsy for PGD. However, use of this method raises concerns regarding the potential developmental damage caused to embryos from the heat generated by the laser, in addition to the removal of blastomeres. The objective of this study was to compare embryo development from day 3 through day 5 between cycles with and without laser-assisted biopsy for PGD within the same patients.DesignRetrospective study.Materials and methodsA total of 20 patients underwent both cycles without biopsy and with laser-assisted embryo biopsy for PGD between February 2002 and February 2004. Biopsy (with removal of 1–2 blastomeres) was performed on day 3 after oocyte retrieval and in vitro fertilization. Zona pellucida dissection was undertaken with a 1.48 micrometer wavelength infrared laser, using a double pulsed 1.0 microsecond burst with 140 milliwatt power at the target (Zilos Laser System, Hamilton Thorne Research, Beverly, MA). Embryos were transferred on the fifth day of development. Comparison of the following day 3 variables was conducted to assess the comparability of embryo cohorts between biopsy and control groups: number of embryos; mean cell number per embryo; and mean percentage of fragmentation per embryo. To assess the effect of the biopsy procedure, the following variables were compared between the biopsy and control cycles: the percentage of embryos showing forward progression between day 3 and day 5 (neither arresting nor degenerating) and the percentages of embryos progressing to the stages of compaction, cavitation or blastocyst expansion. Differences between biopsy and control cycles were evaluated by paired sample t-test.ResultsNumber of embryos per cycle was similar between the biopsy and control cycles (11.4 vs 12.7, p = 0.27), as were day 3 fragmentation rates (6.7% vs 6.2%, p = 0.41). Mean day 3 cell number was slightly lower for biopsy compared to control embryos (6.2 vs 6.6, p = 0.021). Proportions of embryos to continue developing after day 3 were similar between biopsy and control embryos (77.3% vs 74.4%, p = 0.48). Among those embryos continuing to develop after day 3, the proportions achieving at least the stages of compaction (89.3% vs 90.0%, p = 0.87), cavitation (43.1% vs 49.8%, p = 0.34), or blastocyst expansion (28.0% vs 27.7%, p = 0.96) by day 5 were similar between biopsy and control embryos.ConclusionUse of patients as their own controls in the evaluation of the effects of the biopsy procedure is a much more reliable method of controlling for patient variability than the use of controls matched based on similar characteristics. This paired sample analysis revealed no differences in embryonic development between biopsy and control embryos after day 3, when biopsies were performed. These results suggest that the biopsy procedure does not significantly reduce the developmental potential of human preimplantation embryos. ObjectiveLaser-assisted techniques facilitate the procedure of embryo biopsy for PGD. However, use of this method raises concerns regarding the potential developmental damage caused to embryos from the heat generated by the laser, in addition to the removal of blastomeres. The objective of this study was to compare embryo development from day 3 through day 5 between cycles with and without laser-assisted biopsy for PGD within the same patients. Laser-assisted techniques facilitate the procedure of embryo biopsy for PGD. However, use of this method raises concerns regarding the potential developmental damage caused to embryos from the heat generated by the laser, in addition to the removal of blastomeres. The objective of this study was to compare embryo development from day 3 through day 5 between cycles with and without laser-assisted biopsy for PGD within the same patients. DesignRetrospective study. Retrospective study. Materials and methodsA total of 20 patients underwent both cycles without biopsy and with laser-assisted embryo biopsy for PGD between February 2002 and February 2004. Biopsy (with removal of 1–2 blastomeres) was performed on day 3 after oocyte retrieval and in vitro fertilization. Zona pellucida dissection was undertaken with a 1.48 micrometer wavelength infrared laser, using a double pulsed 1.0 microsecond burst with 140 milliwatt power at the target (Zilos Laser System, Hamilton Thorne Research, Beverly, MA). Embryos were transferred on the fifth day of development. Comparison of the following day 3 variables was conducted to assess the comparability of embryo cohorts between biopsy and control groups: number of embryos; mean cell number per embryo; and mean percentage of fragmentation per embryo. To assess the effect of the biopsy procedure, the following variables were compared between the biopsy and control cycles: the percentage of embryos showing forward progression between day 3 and day 5 (neither arresting nor degenerating) and the percentages of embryos progressing to the stages of compaction, cavitation or blastocyst expansion. Differences between biopsy and control cycles were evaluated by paired sample t-test. A total of 20 patients underwent both cycles without biopsy and with laser-assisted embryo biopsy for PGD between February 2002 and February 2004. Biopsy (with removal of 1–2 blastomeres) was performed on day 3 after oocyte retrieval and in vitro fertilization. Zona pellucida dissection was undertaken with a 1.48 micrometer wavelength infrared laser, using a double pulsed 1.0 microsecond burst with 140 milliwatt power at the target (Zilos Laser System, Hamilton Thorne Research, Beverly, MA). Embryos were transferred on the fifth day of development. Comparison of the following day 3 variables was conducted to assess the comparability of embryo cohorts between biopsy and control groups: number of embryos; mean cell number per embryo; and mean percentage of fragmentation per embryo. To assess the effect of the biopsy procedure, the following variables were compared between the biopsy and control cycles: the percentage of embryos showing forward progression between day 3 and day 5 (neither arresting nor degenerating) and the percentages of embryos progressing to the stages of compaction, cavitation or blastocyst expansion. Differences between biopsy and control cycles were evaluated by paired sample t-test. ResultsNumber of embryos per cycle was similar between the biopsy and control cycles (11.4 vs 12.7, p = 0.27), as were day 3 fragmentation rates (6.7% vs 6.2%, p = 0.41). Mean day 3 cell number was slightly lower for biopsy compared to control embryos (6.2 vs 6.6, p = 0.021). Proportions of embryos to continue developing after day 3 were similar between biopsy and control embryos (77.3% vs 74.4%, p = 0.48). Among those embryos continuing to develop after day 3, the proportions achieving at least the stages of compaction (89.3% vs 90.0%, p = 0.87), cavitation (43.1% vs 49.8%, p = 0.34), or blastocyst expansion (28.0% vs 27.7%, p = 0.96) by day 5 were similar between biopsy and control embryos. Number of embryos per cycle was similar between the biopsy and control cycles (11.4 vs 12.7, p = 0.27), as were day 3 fragmentation rates (6.7% vs 6.2%, p = 0.41). Mean day 3 cell number was slightly lower for biopsy compared to control embryos (6.2 vs 6.6, p = 0.021). Proportions of embryos to continue developing after day 3 were similar between biopsy and control embryos (77.3% vs 74.4%, p = 0.48). Among those embryos continuing to develop after day 3, the proportions achieving at least the stages of compaction (89.3% vs 90.0%, p = 0.87), cavitation (43.1% vs 49.8%, p = 0.34), or blastocyst expansion (28.0% vs 27.7%, p = 0.96) by day 5 were similar between biopsy and control embryos. ConclusionUse of patients as their own controls in the evaluation of the effects of the biopsy procedure is a much more reliable method of controlling for patient variability than the use of controls matched based on similar characteristics. This paired sample analysis revealed no differences in embryonic development between biopsy and control embryos after day 3, when biopsies were performed. These results suggest that the biopsy procedure does not significantly reduce the developmental potential of human preimplantation embryos. Use of patients as their own controls in the evaluation of the effects of the biopsy procedure is a much more reliable method of controlling for patient variability than the use of controls matched based on similar characteristics. This paired sample analysis revealed no differences in embryonic development between biopsy and control embryos after day 3, when biopsies were performed. These results suggest that the biopsy procedure does not significantly reduce the developmental potential of human preimplantation embryos." @default.
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• W2089781348 date "2004-09-01" @default.
• W2089781348 modified "2023-09-29" @default.
• W2089781348 title "Comparison of embryo development between non-biopsy cycles and laser assisted biopsy cycles for Preimplantation Genetic Diagnosis (PGD) within the same patient population" @default.
• W2089781348 doi "https://doi.org/10.1016/j.fertnstert.2004.07.643" @default.
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