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• W2089801329 startingPage "248" @default.
• W2089801329 abstract "In budding yeast mitosis is endonuclear and associated with a very limited condensation of the chromosomes. Despite this partial chromosomal condensation, condensin is conserved and essential for the Saccharomyces cerevisiae mitotic cycle. Here, we investigate the localization of condensin during the mitotic cycle. In addition to a constitutive association with rDNA, we have discovered that condensin is localized to the kinetochore in a cell cycle-dependent manner. Shortly after duplication of the spindle pole body, the yeast equivalent of the centrosome, we observed a local enrichment of condensin colocalizing with kinetochore components. This specific association is consistent with mutant phenotypes of chromosome loss and defective sister chromatid separation at anaphase. During a short period of the cell cycle, we observed, at the single cell level, a spatial proximity of condensin and a cohesin rosette, without colocalization. Furthermore, using a genetic screen we demonstrated that condensin localization at kinetochores is specifically impaired in a mutant for ulp2/smt4, an abundant SUMO protease. In conclusion, during chromosome segregation, we established a SUMO-dependent cell cycle-specific condensin concentration colocalizing with kinetochores." @default.
• W2089801329 created "2016-06-24" @default.
• W2089801329 creator A5006231218 @default.
• W2089801329 creator A5034632534 @default.
• W2089801329 creator A5072855877 @default.
• W2089801329 creator A5088672313 @default.
• W2089801329 date "2008-05-01" @default.
• W2089801329 modified "2023-10-02" @default.
• W2089801329 title "Cell cycle-dependent kinetochore localization of condensin complex in Saccharomyces cerevisiae" @default.
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• W2089801329 doi "https://doi.org/10.1016/j.jsb.2008.01.002" @default.
• W2089801329 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18296067" @default.
• W2089801329 hasPublicationYear "2008" @default.
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