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- W2089807857 abstract "Abstract Abstract A highly specific and sensitive enzymatic assay for dihydrofolate has been developed. The method involves enzymatic conversion of dihydrofolate to methylenetetrahydrofolate with subsequent stoichiometric entrapment of this folate cofactor into a covalent, ternarv complex with thymidylate synthase and tritiated-fluorodeoxyuridylate. The detection limit in tissue extracts was 0.25 pmoles and reference dihydrofolate was quantitatively recovered in the range 0.25 to 2.00 pmoles by this procedure. Key Words: Dihydrofolatefolic acidthymidylate synthasedihydrofolate reductase" @default.
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- W2089807857 date "1984-01-01" @default.
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- W2089807857 title "Enzymatic Determination of Tissue Dihydrofolate" @default.
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- W2089807857 doi "https://doi.org/10.1080/00032718408077198" @default.
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