Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089909432> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2089909432 endingPage "254" @default.
- W2089909432 startingPage "251" @default.
- W2089909432 abstract "The quantity of aspartate, but not of glutamate, synthesized by human diploid fibroblasts (HDF) was inversely proportional to the glucose concentration. Forty hours after confluent cells were refed with media containing 1.5 mM or 55 microM glucose, the aspartate concentration was 8 microM in medium high in glucose and 28 microM in medium low in glucose; glutamate was 350 microM at both glucose levels. The label incorporation from [U-14C] glutamine (0.8 C/ml) into aspartate after 40 hours in 1.5 mM glucose medium was less then 1000 DPM/ml and into glutamate 540,000 DPM/ml. The respective label incorporation into aspartate in 55 microM glucose medium was 118,000 DPM/ml and into glutamate 450,000 DPM/ml. In media with 1.5 mM glucose, label incorporation into both aspartate and glutamate was observed from [6-14C] glucose, [3-14C] pyruvate, and [1-14C] pyruvate. Since [1-14C] pyruvate labeled aspartate in 1.5 mM glucose medium, whereas [U-14C] glutamine did not, the observations support a cystolic pathway of aspartate synthesis from glycolytic intermediates in the presence of glucose. However, when the glucose concentration is decreased, glutamine appears to be the primary carbon source for aspartate synthesis. Therefore, both the quantity and the pathway of aspartate synthesis in HDF are functions of the glucose concentration. It is postulated that increased accumulation of aspartate in the media of HDF at confluency may be explained by decreased availability of acetyl CoA or of NADH." @default.
- W2089909432 created "2016-06-24" @default.
- W2089909432 creator A5031577944 @default.
- W2089909432 creator A5049843236 @default.
- W2089909432 creator A5060555082 @default.
- W2089909432 date "1981-05-01" @default.
- W2089909432 modified "2023-10-16" @default.
- W2089909432 title "Effect of glucose on aspartate and glutamate synthesis by human diploid fibroblasts" @default.
- W2089909432 cites W1528360166 @default.
- W2089909432 cites W1542594322 @default.
- W2089909432 cites W1963804424 @default.
- W2089909432 cites W1978212802 @default.
- W2089909432 cites W2005066263 @default.
- W2089909432 cites W2034905888 @default.
- W2089909432 cites W2102839087 @default.
- W2089909432 cites W2141172423 @default.
- W2089909432 doi "https://doi.org/10.1002/jcp.1041070211" @default.
- W2089909432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6114103" @default.
- W2089909432 hasPublicationYear "1981" @default.
- W2089909432 type Work @default.
- W2089909432 sameAs 2089909432 @default.
- W2089909432 citedByCount "12" @default.
- W2089909432 crossrefType "journal-article" @default.
- W2089909432 hasAuthorship W2089909432A5031577944 @default.
- W2089909432 hasAuthorship W2089909432A5049843236 @default.
- W2089909432 hasAuthorship W2089909432A5060555082 @default.
- W2089909432 hasConcept C170493617 @default.
- W2089909432 hasConcept C185592680 @default.
- W2089909432 hasConcept C20251656 @default.
- W2089909432 hasConcept C2779349466 @default.
- W2089909432 hasConcept C515207424 @default.
- W2089909432 hasConcept C55493867 @default.
- W2089909432 hasConcept C61174792 @default.
- W2089909432 hasConcept C62231903 @default.
- W2089909432 hasConcept C86803240 @default.
- W2089909432 hasConceptScore W2089909432C170493617 @default.
- W2089909432 hasConceptScore W2089909432C185592680 @default.
- W2089909432 hasConceptScore W2089909432C20251656 @default.
- W2089909432 hasConceptScore W2089909432C2779349466 @default.
- W2089909432 hasConceptScore W2089909432C515207424 @default.
- W2089909432 hasConceptScore W2089909432C55493867 @default.
- W2089909432 hasConceptScore W2089909432C61174792 @default.
- W2089909432 hasConceptScore W2089909432C62231903 @default.
- W2089909432 hasConceptScore W2089909432C86803240 @default.
- W2089909432 hasIssue "2" @default.
- W2089909432 hasLocation W20899094321 @default.
- W2089909432 hasLocation W20899094322 @default.
- W2089909432 hasOpenAccess W2089909432 @default.
- W2089909432 hasPrimaryLocation W20899094321 @default.
- W2089909432 hasRelatedWork W1573202329 @default.
- W2089909432 hasRelatedWork W16811898 @default.
- W2089909432 hasRelatedWork W1963548841 @default.
- W2089909432 hasRelatedWork W1979197640 @default.
- W2089909432 hasRelatedWork W2052111512 @default.
- W2089909432 hasRelatedWork W2053283970 @default.
- W2089909432 hasRelatedWork W2058161259 @default.
- W2089909432 hasRelatedWork W2072481708 @default.
- W2089909432 hasRelatedWork W2153438741 @default.
- W2089909432 hasRelatedWork W2217719122 @default.
- W2089909432 hasVolume "107" @default.
- W2089909432 isParatext "false" @default.
- W2089909432 isRetracted "false" @default.
- W2089909432 magId "2089909432" @default.
- W2089909432 workType "article" @default.