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• W2089915212 abstract "Interaction of p50 Rho GTPase-activating protein (p50RhoGAP) with Rho family small GTPases was investigated in a yeast two-hybrid system, by radioactive GAP assay, and in a Rac-regulated enzymatic reaction, through superoxide production by the phagocytic NADPH oxidase. The yeast two-hybrid system revealed an interaction between the C-terminal GAP domain and the N-terminal part of p50RhoGAP. The first 48 amino acids play a special role both in the stabilization of the intramolecular interaction and in recognition of the prenyl tail of small GTPases. The GAP assay and the NADPH oxidase activity indicate that the GTPase-activating effect of full-length p50RhoGAP is lower on non-prenylated than on prenylated small GTPase. Removal of amino acids 1–48 and 169–197 of p50RhoGAP increases the GAP effect on non-prenylated Rac, whereas prenylated Rac reacts equally well with the full-length and the truncated proteins. We suggest that p50RhoGAP is in an autoinhibited conformation stabilized by the stretches 1–48 and 169–197 and the prenyl group of the small GTPase plays a role in releasing this intramolecular restraint. Interaction of p50 Rho GTPase-activating protein (p50RhoGAP) with Rho family small GTPases was investigated in a yeast two-hybrid system, by radioactive GAP assay, and in a Rac-regulated enzymatic reaction, through superoxide production by the phagocytic NADPH oxidase. The yeast two-hybrid system revealed an interaction between the C-terminal GAP domain and the N-terminal part of p50RhoGAP. The first 48 amino acids play a special role both in the stabilization of the intramolecular interaction and in recognition of the prenyl tail of small GTPases. The GAP assay and the NADPH oxidase activity indicate that the GTPase-activating effect of full-length p50RhoGAP is lower on non-prenylated than on prenylated small GTPase. Removal of amino acids 1–48 and 169–197 of p50RhoGAP increases the GAP effect on non-prenylated Rac, whereas prenylated Rac reacts equally well with the full-length and the truncated proteins. We suggest that p50RhoGAP is in an autoinhibited conformation stabilized by the stretches 1–48 and 169–197 and the prenyl group of the small GTPase plays a role in releasing this intramolecular restraint. Small GTPases of the Rho subfamily (Rho, Rac, Cdc42) have a widespread role in the organization of numerous cell functions, such as polarity, motility, shape changes, membrane trafficking, gene expression, or production of toxic oxygen metabolites (1Takai Y. Sasaki T. Matozaki T. Physiol. Rev. 2001; 81: 153-208Crossref PubMed Scopus (2072) Google Scholar, 2Ridley A.J. Trends Cell Biol. 2001; 11: 471-477Abstract Full Text Full Text PDF PubMed Scopus (644) Google Scholar, 3Burridge K. Wennerberg K. Cell. 2004; 116: 167-179Abstract Full Text Full Text PDF PubMed Scopus (1519) Google Scholar). Similarly to other GTP-binding proteins, they are active in the GTP-bound form and inactive in the GDP-bound state. The species of the bound nucleotide is regulated by three different types of proteins. Under physiological conditions, guanine nucleotide exchange factors promote the release of GDP and favor the binding of GTP, guanine nucleotide dissociation inhibitor maintains the small GTPase in its GDP-bound form in the cytosol, and GTPase-activating protein(s) (GAP) 1The abbreviations used are: GAP, GTPase-activating protein; GST, glutathione S-transferase; GMPPNP, guanosine 5′-[β,γ-imido]triphosphate. accelerate the endogenous rate of GTP hydrolysis of the small GTPase (4Boguski M.S. McCormick F. Nature. 1993; 366: 643-654Crossref PubMed Scopus (1762) Google Scholar). According to recent data ∼170 proteins are predicted to function as GAP for various small GTPases of the Ras superfamily, and 70 of these proteins contain a typical Rho/RacGAP domain (5Peck J. Douglas IV, G. Wu C.H. Burbelo P.D. FEBS Lett. 2002; 528: 27-34Crossref PubMed Scopus (144) Google Scholar, 6Bernards A. Biochim. Biophys. Acta. 2003; 1603: 47-82PubMed Google Scholar). Apparently, cells with specialized functions express different subsets of this “GAP repertoire” (7Geiszt M. Dagher M.C. Molnár G. Havasi A. Fauré J. Paclet M.H. Morel F. Ligeti E. Biochem. J. 2001; 355: 851-858Crossref PubMed Scopus (29) Google Scholar). Under in vitro conditions, many of the predicted Rho/RacGAPs interact with several members of the Rho subfamily (6Bernards A. Biochim. Biophys. Acta. 2003; 1603: 47-82PubMed Google Scholar). Acceleration of GTP hydrolysis is based on the participation of a critical arginine of the GAP in the stabilization of the transition state of the substrate binding site of the small GTPase (8Scheffzek K. Ahmadian M.R. Kabsch W. Wiesmuller L. Lautwein A. Schmitz F. Wittinghofer A. Science. 1997; 277: 333-338Crossref PubMed Scopus (1208) Google Scholar). In addition to the GAP domain, Rho/RacGAPs contain a large variety of putative protein and lipid interaction domains, which could modify the localization, activity, or substrate specificity of the relevant GAP. However, our knowledge on the details of these interactions and on their functional consequences is scarce (9Bernards A. Settleman J. Trends Cell Biol. 2004; 14: 377-385Abstract Full Text Full Text PDF PubMed Scopus (239) Google Scholar). p50RhoGAP (also called Cdc42GAP) is a 50-kDa protein that is widely expressed in many tissues. The typical GAP domain is located near to the C-terminal end of the protein preceded by a short proline-rich sequence and by a domain that is homologous to Sec14p, a phospholipid transfer protein in Saccharomyces cerevisiae (Sec14 or BCH domain) (10Low B.C. Seow K.T. Guy G.R. J. Biol. Chem. 2000; 275: 14415-14422Abstract Full Text Full Text PDF PubMed Scopus (31) Google Scholar). In a previous study we have shown that the isolated GAP domain reacted significantly better both with Rac and Rho than the full-length protein did. We suggested an intramolecular interaction that could limit the accessibility of the GAP domain (11Molnar G. Dagher M.C. Geiszt M. Settleman J. Ligeti E. Biochemistry. 2001; 40: 10542-10549Crossref PubMed Scopus (64) Google Scholar). The aim of the present investigations was to gain insight into the details of the proposed interaction within the p50RhoGAP molecule. On the basis of two-hybrid interaction experiments, GAP assays, and tests of the activity of a Rac-regulated enzyme complex we show that two different parts of the p50RhoGAP participate in the shielding of the GAP domain. Interaction with the prenyl moiety of the small GTPase releases this restraint within the GAP molecule. Materials—Ampicillin, bovine serum albumin, components of the LB medium, dimethyl sulfoxide, EGTA, ferricytochrome c (horse heart, type VI), flavin adenine dinucleotide, glutathione, glutathione-agarose, NADPH, 1,2-didecanoyl-sn-glycerol 3-phosphate (phosphatidic acid), HEPES, sucrose, and 1-oleoyl-2-acetylglycerol were purchased from Sigma. Superoxide dismutase and GTP were from Roche Applied Science, isopropyl β-d-1-thiogalactopyranoside was from Promega. [γ-32P]GTP was obtained from the Institute of Isotopes, Budapest, Hungary. All the other reagents were of highest available quality. Reagents for the yeast two-hybrid system were a gift of Dr. J. Camonis, Institut Curie, Paris. Plasmid Constructs—The cDNA of various fragments of p50RhoGAP was inserted into the pBTM116 vector in-frame with the LexA DNA-binding protein yielding the Lexp50RhoGAP constructs summarized in Fig. 1A. Alternatively, the cDNA of full-length p50RhoGAP or its GAP domain (amino acids 252–439) were inserted in-frame with the activation domain of Gal4 in pGAD vector. The wild-type and the C189G non-prenylatable Rac1 mutant were also cloned into pGAD vector (12Di-Poi ̈ N. Fauré J. Grizot S. Molnár G. Pick E. Dagher M.-C. Biochemistry. 2001; 40: 10014-10022Crossref PubMed Scopus (75) Google Scholar), and these constructs served as templates to prepare the prenylatable and non-prenylatable mutants Q61L and T17N using a commercial mutagenesis kit (Stratagene). The constructs for the GST fusion protein of the GAP domain (198GAP) and the full-length p50RhoGAP, as well as Rac1, RhoA, and Cdc42 were generous gifts of Professor Alan Hall. The cDNA of the different fragments of p50RhoGAP (see Fig. 1B) was prepared by PCR using the GST-p50RhoGAP construct as template, and they were cloned in-frame into pGEX-4T2 vector. All plasmid constructs have been sequenced. The Escherichia coli clones producing p47phox and p67phox were a generous gift of Dr. F. Wientjes Expression of Proteins—The expression of GST fusion proteins in E. coli was induced by 0.5 mm isopropyl β-d-1-thiogalactopyranoside and the proteins were purified as described in Ref. 13Self A. Hall A. Methods Enzymol. 1995; 256: 3-10Crossref PubMed Scopus (167) Google Scholar. Prenylated Rac1 was isolated from the membrane fraction of Sf9 cells and purified as described in Ref. 12Di-Poi ̈ N. Fauré J. Grizot S. Molnár G. Pick E. Dagher M.-C. Biochemistry. 2001; 40: 10014-10022Crossref PubMed Scopus (75) Google Scholar. Cytochrome b558 was purified and relipidated as described in Ref. 14Batot G. Paclet M.H. Doussiere J. Vergnaud S. Martel C. Vignais P.V. Morel F. Biochim. Biophys. Acta. 1998; 1406: 188-202Crossref PubMed Scopus (39) Google Scholar. Yeast Two-hybrid System—The two-hybrid system described in Ref. 15Fuchs A. Dagher M.C. Jouan A. Vignais P.V. Eur. J. Biochem. 1994; 226: 587-595Crossref PubMed Scopus (38) Google Scholar was used. Briefly, L40 yeast strain was transformed with the two plasmids using classical LiAc protocol. Doubly transformed yeast cells were selected on a medium lacking Trp and Leu. The reporter genes HIS3 and LacZ were assayed by growth on a medium lacking Trp, Leu, and His and by monitoring β-galactosidase activity in a filter test, respectively (16Transy C. Legrain P. Mol. Biol. Rep. 1995; 21: 119-127Crossref PubMed Scopus (30) Google Scholar). For a quantitative assay, the microplate test was used as described in Ref. 17Brouchon-Macari L. Joseph M.C. Dagher M.C. BioTechniques. 2003; 35: 446-448Crossref PubMed Scopus (6) Google Scholar. The expression level of all fusion proteins was controlled by appropriate antibodies obtained from Clontech. Measurement of GTP Hydrolysis of Small G Proteins—The nitrocellulose filter binding assay was applied as described in Ref. 18Self A. Hall A. Methods Enzymol. 1995; 256: 67-76Crossref PubMed Scopus (115) Google Scholar. The loading of the GTPase (1–4 μg of E. coli or Sf9 protein) was performed with high specific activity (3000 Ci/mmol) [γ-32P]GTP in low magnesium buffer (16 mm Tris-Cl, pH 7.5, 20 mm NaCl, 0.1 mm dithiothreitol, 5 mm EDTA, and 100 nm [γ-32P]GTP (5 μCi)) for 5 min at room temperature and stopped by adding 20 mm MgCl2. The loaded G proteins were kept on ice to decrease nucleotide hydrolysis. The GTPase reaction was initiated by addition of 3 μl of loaded G protein to 27 μl of reaction mixture (20 °C) containing 16 mm Tris-Cl, pH 7.5, 0.1 mm dithiothreitol, 1 mg/ml bovine serum albumin, 1 mm unlabeled GTP, and the investigated protein with GAP activity. Aliquots of 5 μl were taken at regular intervals and filtered through nitrocellulose filters. The filters were washed three times with 2 ml of ice-cold buffer consisting of 50 mm Tris-Cl, pH 7.7, and 5 mm MgCl2. The radioactivity was measured in distilled water on the basis of the Cerenkov effect in a Beckman LS 5000TD liquid scintillation spectrometer. Data are presented as the decrease in time of protein-bound radioactivity retained on the filters. Measurement of Superoxide Generation in the Cell-free System—The rate of superoxide generation was determined as the superoxide dismutase-inhibitable reduction of ferricytochrome c measured at 550 nm in a Labsystems iEMS Microplate Reader. In the fully purified system 0.005 μg of purified and relipidated cytochrome b558 (0.6 nm) was incubated with 0.5 μg of p47phox (87 nm), 0.5 μg of p67phox (67 nm), 0.12 μg of GTP-loaded prenylated or non-prenylated Rac (60 nm), 125 μm 1-oleoyl-2-acetylglycerol, 187.5 μm phosphatidic acid, and 125 μm cytochrome c in a final volume of 80 μl. Superoxide (O2·¯) production was initiated by the addition of 200 μm NADPH and followed for 20 min. For loading of Rac with GTP, 1.8 μg of the small GTPase was incubated in a final volume of 22 μl of phosphate-buffered saline, containing 4 mm EDTA and 30 μm GTP, for 10 min at 25 °C. Subsequently 20 mm MgCl2 was added, and the protein was kept on ice. Demonstration of Intramolecular Interactions in p50RhoGAP in a Yeast Two-hybrid System—To investigate potential intramolecular interactions, we cloned the isolated GAP domain in pGAD vector and tested its reaction with various fragments of the protein cloned into pLex vector (Fig. 1A). As summarized in Table I, we found an interaction between the GAP domain and 1–255GAP, a fragment containing the N-terminal end of the protein plus the Sec14 domain plus the proline-rich region. Interestingly, no interaction was detectable with the Sec14/proline-rich region or the Sec14 fragment, i.e. in the absence of the N-terminal end of the molecule. Interaction of the isolated N-terminal part of p50RhoGAP with the GAP domain could not be directly tested, as the 1–51 fragment induced strong non-specific transactivation of the reporter genes. On the other hand, in the two-hybrid system we could not reveal any interaction between the fragment 1–168 and the GAP domain, suggesting that the N-terminal 48 amino acids are not sufficient for the establishment of the intramolecular binding. (We verified that all constructs were correctly expressed.) These results suggest that amino acids both on the N-terminal and on the C-terminal end of the fragment 1–255 may be involved in the stabilization of the intramolecular interaction within the p50RhoGAP molecule. Apparently, in the full-length protein the critical domains are masked as the isolated GAP domain did not interact either with full-length p50RhoGAP or with the fragment lacking the first 48 amino acids (49GAP) (Table I).Table IInteractions of the GAP domain with different p50RhoGAP constructspGADpLEXFull-length GAP49GAP1-255GAP1-168GAPSec14/PRRSec14p50GAP domain--+--- Open table in a new tab Investigation of the Interaction of p50RhoGAP with Rac in the Two-hybrid System—In the two-hybrid system there was no detectable reaction between full-length p50RhoGAP and wild-type Rac1 (Table II). We did not find any reaction with wild-type Rac2 either (data not shown). Probably the interaction between the GDP form of this small GTPase and p50RhoGAP is not sufficiently stable to allow detection in the two-hybrid system. In contrast, the constitutively active mutant Q61LRac1 reacted with full-length p50RhoGAP and so did the T17NRac1 mutant. 2Experiments carried out in our laboratories indicate that Rac1T17N is either present in the cell partially in GTP-bound form or exhibits a special conformation mimicking the GTP-bound state of wild-type Rac1. Detailed data will be published separately. Interestingly, both mutants were only able to interact with the full-length protein, the absence of the first 48 amino acids prevented the interaction. No reaction was detectable between wild-type or mutant Rac1 and any other fragment of p50RhoGAP either. Thus, the first 48 amino acids of p50RhoGAP have a decisive role in the stabilization of the interaction of this GAP with its substrate, Rac.Table IIInteraction of p50RhoGAP constructs with prenylated Rac GTPasespGADpLEXFull-length GAP49GAP1-255GAPSec14/PRRSec14pRac1-----pQ61L+----pT17N+---- Open table in a new tab Next we asked whether the prenylation state of the small GTPase has a role in the interaction with p50RhoGAP. To this end we prepared C189GRac1, Q61L, and T17N mutants, where the absence of the cysteine from the C-terminal CAAX box of Rac prevents its prenylation in the yeast cell. In a quantitative β-galactosidase assay, wild-type Rac1 did not show higher activity than the empty vector, and this feature was independent of the prenylation state of the small GTPase (Fig. 2). This finding is in accordance with our data obtained on the filter (Table II). In contrast, in the case of both the Q61L and the T17N mutants, a significant difference was observed whether the protein was prenylated or not. The activity measured with the non-prenylated proteins did not exceed the background value, whereas with pQ61LRac1 and pT17NRac1 a 2.2- and 1.7-fold increase was detected, respectively. Thus, the prenyl moiety seems to participate actively in the stabilization of the interaction of Rac with p50RhoGAP. Effect of p50RhoGAP Fragments on GTP Hydrolysis by Rac—To test our results obtained in the two-hybrid system, we expressed the corresponding fragments of p50RhoGAP as GST fusion proteins (Fig. 1B) and studied their GTPase-activating effect in a classical radioactive GAP assay (Fig. 3). In accordance with our earlier finding (11Molnar G. Dagher M.C. Geiszt M. Settleman J. Ligeti E. Biochemistry. 2001; 40: 10542-10549Crossref PubMed Scopus (64) Google Scholar), full-length p50RhoGAP had only a weak GAP effect on non-prenylated Rac1 prepared from bacteria. Removal of the first 48 amino acids improved its reaction, although it did not attain that of the isolated GAP domain (198GAP) (Fig. 3A). The difference in the reactivity of the three constructs of p50RhoGAP was not affected by the presence of geranylgeranyl pyrophosphate in equimolar ratio to non-prenylated Rac1 (data not shown). When GTP hydrolysis by prenylated Rac was investigated, the full-length protein and the two truncated forms proved to have similar efficiency (Fig. 3B). Next we wanted to gain information on which part of the Sec14 domain could be responsible for the restriction of the interaction of 49GAP with non-prenylated Rac. In deciding on the site of further truncations, we were led by two considerations. 1) The sequences participating in lipid binding of the Saccharomyces protein Sec14p correspond to the stretches 96–100, 105–115, 140–145, and 170–180 of p50RhoGAP (19Aravind L. Neuwald A.F. Ponting C.P. Curr. Biol. 1999; 9: 195-197Abstract Full Text Full Text PDF PubMed Google Scholar), and 2) the sequence between amino acids 135–170 shows weak homology to an immunoglobulin domain and in some proteins (e.g. Rho-guanine nucleotide dissociation inhibitor, Trio) an Ig domain was shown to interact with the prenyl group (20Hoffman G.R. Nassar N. Cerione R.A. Cell. 2000; 100: 345-356Abstract Full Text Full Text PDF PubMed Scopus (415) Google Scholar, 21Medley Q.G. Serra-Pages C. Iannott E. Seipel K. Tang M. O'Brien S.P. Streuli M. J. Biol. Chem. 2000; 275: 36116-36123Abstract Full Text Full Text PDF PubMed Scopus (30) Google Scholar). Thus, we prepared GST fusion proteins starting at amino acid 86, 121, or 169 (Fig. 1B). In the GAP assay all of these fragments behaved similarly to 49GAP (Table III). The fact that they were less effective than fragment 198GAP in stimulating GTP hydrolysis on non-prenylated Rac, indicates that amino acids 169–197 play a role in the interaction of p50RhoGAP with prenylated small GTPases. On the other hand, truncation of the molecule beyond the fragment 198GAP did not improve its reaction with non-prenylated Rac1 (data not shown). Thus, our GAP assays provided the information that two different parts of the p50RhoGAP molecule, namely the N-terminal 48 amino acids and the stretch between amino acids 169 and 197 at the C-terminal end of the Sec14 domain, are involved in the structural changes induced by prenylated Rac1. It should be remarked, that the above fragments of p50RhoGAP gave identical reactions with non-prenylated RhoA and Cdc42 as detailed for Rac1 (data not shown).Table IIIEffect of different fragments of p50RhoGAP on the GTP hydrolysis rate of Rac1ControlFull-length GAP49GAP86GAP121GAP169GAP198GAPnpRac81.6 ± 1.458.5 ± 3.144.4 ± 1.644.6 ± 2.444.2 ± 3.841.8 ± 2.713.1 ± 1.5pRac69.2 ± 4.724.0 ± 2.426.6 ± 4.325 ± 2.927.5 ± 5.229.5 ± 5.124.1 ± 0.9 Open table in a new tab Effect of p50RhoGAP Fragments on the Activation of NADPH Oxidase—We wanted to substantiate the results obtained in the GAP assay in a functional test where participation of the GTP-bound form of Rac is decisive. The NADPH oxidase of phagocytic cells is a multisubunit enzyme (for review see Ref. 22Vignais P.V. Cell Mol. Life Sci. 2002; 59: 1428-1459Crossref PubMed Scopus (634) Google Scholar), the assembly and catalytic activity of which depends on the prevalence of Rac in the GTP-bound active form (23Moskwa P. Dagher M.C. Paclet M.H. Morel F. Ligeti E. Biochemistry. 2002; 41: 10710-10716Crossref PubMed Scopus (20) Google Scholar). We applied the fully purified version of the cell-free activation system of this enzyme, consisting of purified and relipidated cytochrome b558 and recombinant p47phox, p67phox, and GTP-loaded prenylated or non-prenylated Rac (Fig. 4). In our earlier experiments (7Geiszt M. Dagher M.C. Molnár G. Havasi A. Fauré J. Paclet M.H. Morel F. Ligeti E. Biochem. J. 2001; 355: 851-858Crossref PubMed Scopus (29) Google Scholar), we showed that this system does not contain any GAP activity, allowing a high rate of O2·¯ production in the presence of GTP (Fig. 4, control) that is independent of the prenylation state of Rac (Fig. 4, compare A with B). The addition of full-length p50RhoGAP to the activation system reduced the rate of O2·¯ production, indicating the GTPase-activating effect on Rac. When non-prenylated Rac was used, 49GAP was more effective than the full-length p50RhoGAP, and the isolated GAP domain was more effective than 49GAP (Fig. 4A). The fragments 86GAP, 121GAP, and 169GAP behaved again similar to 49GAP. In contrast, in the case of prenylated Rac, all the different fragments of p50RhoGAP had the same efficiency (Fig. 4B). Thus, the results indicating the activity of RacGTP in a complex enzymatic system fully support the data obtained in the GAP assay. The crystal structure of the GAP domain of p50RhoGAP has been resolved both in uncomplexed state (24Barrett T. Xiao B. Dodson E.J. Dodson G. Ludbrook S.B. Nurmahomed K. Gamblin S.J. Musacchio A. Smerdon S.J. Eccleston J.F. Nature. 1997; 385: 458-461Crossref PubMed Scopus (98) Google Scholar) and in complex with Cdc42Hs-GMPPNP, representing the ground state of the small GTPase (25Rittinger K. Walker P.A. Eccleston J.F. Nurmahomed K. Owen D. Laue E. Gamblin S.J. Smerdon S.J. Nature. 1997; 388: 693-696Crossref PubMed Scopus (223) Google Scholar) and with Rho-GDPAlF4, representing the transition state during hydrolysis of GTP (26Rittinger K. Walker P.A. Eccleston J.F. Smerdon S.J. Gamblin S.J. Nature. 1997; 389: 758-762Crossref PubMed Scopus (355) Google Scholar). These studies clearly showed the decisive role of Arg-85 of the GAP domain as in the transition state it participates in the active site of the small GTPase. However, these studies were carried out with the isolated GAP domain (fragment 198–439) and with small GTPases expressed as GST fusion proteins in E. coli, thus, without the posttranslational modifications typical for expression in eukaryotic cells, isoprenylation, truncation, and methylation (4Boguski M.S. McCormick F. Nature. 1993; 366: 643-654Crossref PubMed Scopus (1762) Google Scholar). The crystal structure of the S. cerevisiae phosphatidylinositol transfer protein (Sec14) is also available (27Sha B. Phillips S.E. Bankaitis V.A. Luo M. Nature. 1998; 391: 506-510Crossref PubMed Scopus (232) Google Scholar), and this protein shows sequence homology with the N-terminal part of p50RhoGAP. However, at present there is no structural information either on the relation of the two parts of the p50RhoGAP molecule or on the possible role of the prenyl group in the interaction with its substrate, although in the cellular milieu the true interacting partners are full-length p50RhoGAP and prenylated small GTPases. To our knowledge this is the first study trying to decipher on a functional basis the possible intramolecular interactions within p50RhoGAP and its relation to the prenylated small GTPase. Data obtained in the yeast two-hybrid system revealed an important role of the N-terminal 48 amino acids of p50RhoGAP both in establishing intramolecular interaction with the C-terminal GAP domain (Table I) and in the stable interaction with Rac (Table II). Furthermore, the two-hybrid experiments indicated that the positive interaction between Rac and p50RhoGAP depends also on the prenyl moiety of the small GTPase (Fig. 2). Based on these data we carried out a direct investigation of the role of the N-terminal 48 amino acids in GAP assays, which fully supported the results of the two-hybrid study. The removal of the N-terminal 48 amino acids increases the GTPase-activating action of p50RhoGAP on non-prenylated Rac (and Rho and Cdc42), whereas it does not influence the effect upon prenylated Rac (Fig. 3). The same observation was made in the activation of NADPH oxidase, a complex enzymatic reaction that depends on the prevalence of Rac in the GTP-bound form (Fig. 4). Thus, the first 48 amino acids of p50RhoGAP play a critical role in recognition of the prenyl moiety and increasing the accessibility of the GAP domain to prenylated small GTPase. However, some findings suggest that the removal of the N-terminal 48 amino acids does not interrupt the intramolecular interactions within the p50RhoGAP molecule completely. 1) In the two-hybrid system, positive interaction with the isolated GAP domain required also the contribution of amino acids on the C-terminal end of the Sec14 domain (Table I). 2) Both the GAP assay and the NADPH oxidase test indicated that the removal of amino acids 169–197 resulted in a further increase of the GAP activity of the relevant fragment on the non-prenylated GTPases whereas no change was observed in the reaction with prenylated Rac (Figs. 3 and 4). Apparently, this stretch of amino acids is also involved in recognition of the prenyl moiety. On the basis of our experimental data we propose the model presented in Fig. 5. In the basic state p50RhoGAP is in a closed conformation, where the N-terminal 48 amino acids and the stretch 169–197 at the C-terminal end of the Sec14 domain are involved in the intramolecular interaction restricting the accessibility of the GAP domain. The prenylated small GTPase is able to release the intramolecular restraint and to bring the protein into a more open conformation. In this state the essential amino acid(s) of the GAP domain have free access to the active site of the small GTPase. The following experimental findings indicate that the prenyl group by itself is not sufficient for releasing the autoinhibited state. 1) Soluble geranylgeranyl pyrophosphate is not able to replace the prenyl tail covalently attached to the small GTPase, and 2) prenylated wild-type Rac1 or Rac2 is not able to interact with p50RhoGAP in the two-hybrid system. The proposed model provides another example that the prenyl moiety of the small GTPase plays a significant role not only in the localization of the molecule to hydrophobic surfaces, but also in the interaction with certain proteins (20Hoffman G.R. Nassar N. Cerione R.A. Cell. 2000; 100: 345-356Abstract Full Text Full Text PDF PubMed Scopus (415) Google Scholar, 21Medley Q.G. Serra-Pages C. Iannott E. Seipel K. Tang M. O'Brien S.P. Streuli M. J. Biol. Chem. 2000; 275: 36116-36123Abstract Full Text Full Text PDF PubMed Scopus (30) Google Scholar, 28Grizot S. Faure J. Fieschi F. Vignais P.V. Dagher M.C. Pebay-Peyroula E. Biochemistry. 2001; 40: 10007-10013Crossref PubMed Scopus (111) Google Scholar, 29An Y. Shao Y. Alory C. Matteson J. Sakisaka T. Chen W. Gibbs R.A. Wilson I.A. Balch W.E. Structure (Camb). 2003; 11: 347-357Abstract Full Text Full Text PDF PubMed Scopus (44) Google Scholar, 30Rak A. Pylypenko O. Durek T. Watzke A. Kushnir S. Brunsveld L. Waldmann H. Goody R.S. Alexandrov K. Science. 2003; 302: 646-650Crossref PubMed Scopus (174) Google Scholar). Suggesting an intramolecular interaction as means of regulation of the activity of a GAP protein is not unprecedented. In the case of p120RasGAP, the N-terminal PH domain was suggested to bind to the C-terminal catalytic domain and inhibit the GAP action of the latter (31Drugan J.K. Rogers-Graham K. Gilmer T. Campbell S. Clark G.J. J. Biol. Chem. 2000; 275: 35021-35027Abstract Full Text Full Text PDF PubMed Scopus (37) Google Scholar). Also for the Rho/Rac/Cdc42GAP oligophrenin-1 it has been recently shown that the N-terminal part of the molecule inhibits its RhoGAP activity in fibroblasts (32Fauchereau F. Herbrand U. Chafey P. Eberth A. Koulakoff A. Vinet M.C. Ahmadian M.R. Chelly J. Billuart P. Mol. Cell. Neurosci. 2003; 23: 574-586Crossref PubMed Scopus (85) Google Scholar). In in vitro studies, we had similar indications for p190RhoGAP (11Molnar G. Dagher M.C. Geiszt M. Settleman J. Ligeti E. Biochemistry. 2001; 40: 10542-10549Crossref PubMed Scopus (64) Google Scholar). Autoinhibition released by the small GTPase is a common phenomenon among the targets of different Rho family proteins (33Bishop A.L. Hall A. Biochem. J. 2000; 348: 241-255Crossref PubMed Scopus (1682) Google Scholar, 34Kim A.S. Kakalis L.T. Abdul-Manan N. Liu G.A. Rosen M.K. Nature. 2000; 404: 151-158Crossref PubMed Scopus (625) Google Scholar). Recent data also indicate that multiple domains of the effector protein and regions of the GTPase outside of the classical switch I and II are involved in the interaction of Rho family GTPases with their target proteins (35Blumenstein L. Ahmadian M.R. J. Biol. Chem. 2004; 279: 53419-53426Abstract Full Text Full Text PDF PubMed Scopus (62) Google Scholar). Further studies have to decide the similarities in the reaction of small GTPases with their target versus regulatory proteins and, also, how widespread autoinhibition is among GAPs for different subfamilies of the small GTPases. Our studies call attention to the prenyl moiety as a possible additional region of the interaction between small GTPases and their relevant GAPs. We thank Marie-Claire Joseph, Laurence Macari, Edit Fedina, Erzsébet Seres-Horváth and Krisztina Süto for devoted and expert technical assistance." @default.
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• W2089915212 date "2005-02-01" @default.
• W2089915212 modified "2023-09-27" @default.
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