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- W2089918685 abstract "Amplified Eptatretus okinoseanus cDNA was digested with NdeI and EcoRI, cloned into pCold trigger factor (TF), and transformed with Escherichia coli strain BL 21 in which a csp A promoter was introduced to inhibit the expression of foreign peptides. Recombinant lactate dehydrogenase (LDH) was obtained in the soluble fraction after sonication of the cells. The protein was digested by HRC 3C protease, thrombin, and factor Xa. The specific activity of TF-tagged protein and tagless protein were mIU/mg and mIU/mg, respectively. The deletion of the TF tag enhanced the activity compared with the native protein to mIU/mg, showing that this expression method is effective for the mass production of the protein to allow further study of the structure of LDH." @default.
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- W2089918685 date "2011-06-30" @default.
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- W2089918685 title "Expression of the Lactate Dehydrogenase Gene from Eptatretus okinoseanus in Escherichia coli" @default.
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- W2089918685 doi "https://doi.org/10.5402/2011/835274" @default.
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