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• W2089945439 endingPage "1396" @default.
• W2089945439 startingPage "1392" @default.
• W2089945439 abstract "HPRT gene mutants were isolated from untreated and x-irradiated cultures of primary human fibroblasts, and mutants carrying large deletions were identified. The breakpoints of the deletions were mapped by methods based on the polymerase chain reaction, and the deletion junctions of four different mutants were sequenced. Alu repeats were associated with one end of three of these junctions, but in each case repeat sequences were not found at the other end. Sequence features found at the deletion breakpoints included in particular short direct and inverted repeats, which may mispair to promote illegitimate recombination. One mutant had additional bases inserted at the deletion junction; these bases formed a direct repeat with a sequence immediately adjacent to the junction, suggesting a mechanism of templated repair of broken DNA in deletion formation." @default.
• W2089945439 created "2016-06-24" @default.
• W2089945439 creator A5038153503 @default.
• W2089945439 creator A5091583144 @default.
• W2089945439 date "1993-02-15" @default.
• W2089945439 modified "2023-09-28" @default.
• W2089945439 title "Formation of large deletions by illegitimate recombination in the HPRT gene of primary human fibroblasts." @default.
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• W2089945439 doi "https://doi.org/10.1073/pnas.90.4.1392" @default.
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