Matches in SemOpenAlex for { <https://semopenalex.org/work/W2089978061> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2089978061 abstract "Research on Leishmania-macrophage interaction is mainly focused on the impact of the parasite on macrophages and several known virulent factors have been described. Furthermore, studies on macrophage revealed several defense mechanisms including various cytokines which are released by macrophages to defend against parasite. In the present study, a new aspect of this interaction was evaluated: parasite characteristics, which emerge when they were cocultivated with macrophage. Two promastigote characteristics, survival at high temperature (32 degrees C) and infectivity rate were the focus of this study. In this study, an in vitro coculture model for promastigotes with macrophage cell line, J774 A1, was introduced using a cell culture chamber system which separates both cell types by a microporous polycarbonate membrane. After 5-7 days of coculturing at 32 degrees C, a few promastigotes survived longer than control group. Once this population of parasite was cultured at optimal temperature (26 degrees C), the emerged new clone was much more infective for J774 A1 cell line in comparison with the original one. Having this system and using the new clone of promastigotes, parasite infectivity rate was raised from 1-2% of original clone to 35-45%. Using this new introduced technique, infective promastigotes were isolated from 9 month old frequently sub-cultured clone of Leishmania major. This coculturing system allows investigators to prepare infective promastigotes from the frequently cultured parasites. Molecular and biochemical mechanisms of this phenomenon need to be investigated." @default.
- W2089978061 created "2016-06-24" @default.
- W2089978061 creator A5020002632 @default.
- W2089978061 creator A5048943497 @default.
- W2089978061 date "2005-12-01" @default.
- W2089978061 modified "2023-09-26" @default.
- W2089978061 title "Isolation of infective promastigotes of Leishmania major from long-term culture by cocultivation with macrophage cell line" @default.
- W2089978061 cites W1966835460 @default.
- W2089978061 cites W1981588164 @default.
- W2089978061 cites W2012423405 @default.
- W2089978061 cites W2019692821 @default.
- W2089978061 cites W2041711772 @default.
- W2089978061 cites W2068469796 @default.
- W2089978061 cites W2068668556 @default.
- W2089978061 cites W2086208209 @default.
- W2089978061 cites W2108516350 @default.
- W2089978061 cites W2125575889 @default.
- W2089978061 cites W2132617233 @default.
- W2089978061 cites W2140670981 @default.
- W2089978061 cites W2145825761 @default.
- W2089978061 cites W2172247061 @default.
- W2089978061 doi "https://doi.org/10.1016/j.biologicals.2005.06.002" @default.
- W2089978061 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16168668" @default.
- W2089978061 hasPublicationYear "2005" @default.
- W2089978061 type Work @default.
- W2089978061 sameAs 2089978061 @default.
- W2089978061 citedByCount "5" @default.
- W2089978061 countsByYear W20899780612019 @default.
- W2089978061 countsByYear W20899780612020 @default.
- W2089978061 crossrefType "journal-article" @default.
- W2089978061 hasAuthorship W2089978061A5020002632 @default.
- W2089978061 hasAuthorship W2089978061A5048943497 @default.
- W2089978061 hasConcept C104317684 @default.
- W2089978061 hasConcept C106358424 @default.
- W2089978061 hasConcept C136764020 @default.
- W2089978061 hasConcept C159047783 @default.
- W2089978061 hasConcept C202751555 @default.
- W2089978061 hasConcept C2522874641 @default.
- W2089978061 hasConcept C2779244956 @default.
- W2089978061 hasConcept C2780599195 @default.
- W2089978061 hasConcept C2781092759 @default.
- W2089978061 hasConcept C2908647359 @default.
- W2089978061 hasConcept C41008148 @default.
- W2089978061 hasConcept C54355233 @default.
- W2089978061 hasConcept C55493867 @default.
- W2089978061 hasConcept C60987743 @default.
- W2089978061 hasConcept C71924100 @default.
- W2089978061 hasConcept C71928629 @default.
- W2089978061 hasConcept C81089528 @default.
- W2089978061 hasConcept C81885089 @default.
- W2089978061 hasConcept C86803240 @default.
- W2089978061 hasConcept C89423630 @default.
- W2089978061 hasConcept C99454951 @default.
- W2089978061 hasConceptScore W2089978061C104317684 @default.
- W2089978061 hasConceptScore W2089978061C106358424 @default.
- W2089978061 hasConceptScore W2089978061C136764020 @default.
- W2089978061 hasConceptScore W2089978061C159047783 @default.
- W2089978061 hasConceptScore W2089978061C202751555 @default.
- W2089978061 hasConceptScore W2089978061C2522874641 @default.
- W2089978061 hasConceptScore W2089978061C2779244956 @default.
- W2089978061 hasConceptScore W2089978061C2780599195 @default.
- W2089978061 hasConceptScore W2089978061C2781092759 @default.
- W2089978061 hasConceptScore W2089978061C2908647359 @default.
- W2089978061 hasConceptScore W2089978061C41008148 @default.
- W2089978061 hasConceptScore W2089978061C54355233 @default.
- W2089978061 hasConceptScore W2089978061C55493867 @default.
- W2089978061 hasConceptScore W2089978061C60987743 @default.
- W2089978061 hasConceptScore W2089978061C71924100 @default.
- W2089978061 hasConceptScore W2089978061C71928629 @default.
- W2089978061 hasConceptScore W2089978061C81089528 @default.
- W2089978061 hasConceptScore W2089978061C81885089 @default.
- W2089978061 hasConceptScore W2089978061C86803240 @default.
- W2089978061 hasConceptScore W2089978061C89423630 @default.
- W2089978061 hasConceptScore W2089978061C99454951 @default.
- W2089978061 hasLocation W20899780611 @default.
- W2089978061 hasLocation W20899780612 @default.
- W2089978061 hasOpenAccess W2089978061 @default.
- W2089978061 hasPrimaryLocation W20899780611 @default.
- W2089978061 hasRelatedWork W1991258842 @default.
- W2089978061 hasRelatedWork W2023207791 @default.
- W2089978061 hasRelatedWork W2042072042 @default.
- W2089978061 hasRelatedWork W2089978061 @default.
- W2089978061 hasRelatedWork W2129443427 @default.
- W2089978061 hasRelatedWork W2360473132 @default.
- W2089978061 hasRelatedWork W2402993141 @default.
- W2089978061 hasRelatedWork W2923646916 @default.
- W2089978061 hasRelatedWork W4240417676 @default.
- W2089978061 hasRelatedWork W2613865554 @default.
- W2089978061 isParatext "false" @default.
- W2089978061 isRetracted "false" @default.
- W2089978061 magId "2089978061" @default.
- W2089978061 workType "article" @default.