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- W2090019672 abstract "Summary A method is described for the preservation of red cells in the frozen state. By freezing in liquid nitrogen, it is possible to obtain after freezing and thawing, high recoveries of red cells with a low concentration of glycerol as a protective additive. Without special equipment the removal of glycerol from the thawed red cells can be carried out by washing with 16% sorbitol in a 0.9% NaCl solution. The viability of the processed red cells as shown by their metabolic properties, osmotic fragility and posttransfusion survival is very satisfactory. Résumé Une méthode pour la conservation des globules rouges est décrite. Par congélation en azote liquide il est possible d'obtenir un rendement très haut de globules rouges après congélation‐décongélation par Taction protective d'une concentration basse de glycérol. Sans utiliser un appareil spécial il est possible d'éüminer le glycérol des globules rouges décongelés, en se servant de sorbitol à 16% dans une solution NaCl à 0,9%. La viabilité des globules rouges est excellente, ainsi que l'indiquent les propriétés métaboliques, la fragilité osmotique et la survie après transfusion. Zusammenfassung Es wird eine Méthode zur Konservierung von Erythrozyten auf lange Dauer beschrieben. Gefrierung mit flüssigem Stickstoff gibt die Müglichkeit, nach Gefrierung und Auftauen, eine hohe Ausbeute von Erythrozyten zu bekommen, indem eine niedrige Konzentration von Glycerol als schützende Substanz verwendet wird. Ohne spezielle Apparatur kann die Entfernung von Glycerol mit 16% Sorbitol in einer physiologischen Kochsalzlüsung vorgenommen werden. Die Lebensfähigkeit der Erythrozyten wie indiziert durch die metabolischen Eigenschaften, die osmotische Fragilität und das Überleben nach Transfusion ist ausgezeichnet." @default.
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- W2090019672 date "1964-09-10" @default.
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- W2090019672 title "Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen" @default.
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- W2090019672 doi "https://doi.org/10.1111/j.1423-0410.1964.tb03326.x" @default.
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